Hostname: page-component-586b7cd67f-t7czq Total loading time: 0 Render date: 2024-11-26T20:15:19.119Z Has data issue: false hasContentIssue false
  • English
  • Français

Serological studies on group- and species-specific antigens of trachoma and inclusion conjunctivitis (TRIC) agents*†

Published online by Cambridge University Press:  15 May 2009

A. L. Terzin ,
N. A. Vedros  and
J. B. Johnston
A. L. Terzin
Affiliation:
U.S. Naval Medical Research Institute, National Naval Medical Center, Bethesda, Maryland
N. A. Vedros
Affiliation:
U.S. Naval Medical Research Institute, National Naval Medical Center, Bethesda, Maryland
J. B. Johnston
Affiliation:
U.S. Naval Medical Research Institute, National Naval Medical Center, Bethesda, Maryland
Rights & Permissions [Opens in a new window]

Extract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Group-reactive ether soluble psittacosis and boiled mouse pneumonitis antigens were tested in parallel, with 85 serum specimens. The results indicate that the group-specific C.F. antigens of these organisms are indistinguishable when tested against sera of trachoma patients, monkeys infected with trachoma or against sera of other individuals.

Sera of rabbits immunized with viable trachoma-inclusion conjunctivitis (TRIC) organisms, grown in tissue culture, were absorbed with boiled elementary body suspension of mouse pneumonitis agent, which removed the group reactive antibodies, and resulted in a species-specific anti-TRIC serum.

The absorbed and unabsorbed TRIC sera were titrated against purified E.B. suspensions, which were prepared both from yolk and from tissue culture grown organisms, of homologous TRIC strains and from other heterologous Bedsonia organisms.

Results of absorption experiments indicate that group reactive antigens prepared from mouse pneumonitis and psittacosis are indistinguishable by C.F. test from the group-specific component of the TRIC antigens. The species-specific antigen of the TRIC agents was well distinguished from the species-specific antigen of the psittacosis agent. However, the C.F. test did not distinguish the strains isolated from trachoma from those isolated from cases of inclusion conjunctivitis.

The stabilizing effect of sucrose and albumin upon the species-specific C.F. antigen of purified elementary bodies of TRIC organisms was found to be pronounced.

Our attempts to produce a species-specific antigen preparation, free from group component, failed.

We have pleasure in thanking Dr F. B. Gordon for suggestions; Dr B. V. Birtašević, Mr H. R. Dressler, Dr E. S. Murray and Dr A. J. Vargosko for procuring sera of patients or organisms grown in tissue culture; Dr T. A. Strike for the help with statistical computations; HMC P. R. Hill, HM3 C.O. Wiese and Mr B. L. Ward for invaluable technical assistance.

Type
Research Article
Information
Journal of Hygiene , Volume 62 , Issue 2 , June 1964 , pp. 185 - 198
Copyright
Copyright © Cambridge University Press 1964

References

REFERENCES

Bedson, S. P. (1936). Observations bearing on the antigenic composition of psittacosis virus. Brit. J. exp. Path. 17, 109.Google Scholar
Bedson, S. P. (1959). The Harben Lectures. The psittacosis-lymphogranuloma group of infective agents. J. R. Inst. publ. Hlth, 22, 67.Google Scholar
Bedson, S. P., Barwell, C. F., King, E. J. & Bishop, L. W. J. (1949). The laboratory diagnosis of lymphogranuloma venereum. J. clin. Path. 2, 241.CrossRefGoogle ScholarPubMed
Bell, S. D. & Theobald, B. (1962). Differentiation of trachoma strains on the basis of immunization against toxic death of mice. Ann. N.Y. Acad. Sci. 98, 337.CrossRefGoogle ScholarPubMed
Chang, I., Wang, S. & Grayston, J. T. (1962). Antigenic relationship of trachoma virus strains in the mouse toxicity prevention test. In The Biology of the Trachoma Agent. Ann. N.Y. Acad. Sci. 98, 347.CrossRefGoogle Scholar
Collier, L. H. & Sowa, J. (1958). Isolation of trachoma virus in embryonate eggs. Lancet, i, 993.CrossRefGoogle Scholar
Gordon, F. B., Quan, A. L. & Dressler, H. R. (1963). Observations on growth of trachoma strains in cell cultures. (Abstract.) Bact. Proc. p. 132.Google Scholar
Hilleman, M. R. (1955). Serological procedure for detecting psittacosis infection in birds. In Psittacosis, p. 74. Ed. Beaudette, F. R., New Brunswick, New Jersey: Rutgers University Press.Google Scholar
Jenkin, H. M., Ross, M. R. & Moulder, J. W. (1961). Species-specific antigens from the cell walls of the agents of meningopneumonitis and feline pneumonitis. J. Immunol. 86, 123.CrossRefGoogle Scholar
Mayer, M. M., Osler, A. G., Bier, O. G. & Heidelberger, M. (1946). Activating effect of magnesium and other cations on hemolytic function of complement. J. exp. Med. 84, 535.CrossRefGoogle ScholarPubMed
Monsur, K. A. & Barwell, C. F. (1951). Observations on the antigenic relationship between the virus of enzootic abortion of ewes and viruses of the psittacosis-lymphogranuloma group. Brit. J. exp. Path. 32, 414.Google ScholarPubMed
Murray, E. S. (1962). Discussion in The Biology of the Trachoma Agent. Ann. N.Y. Acad. Sci. 98, 325.Google Scholar
Reeve, P. & Taverne, J. (1962). Some properties of the complement-fixing antigens of the agents of trachoma and inclusion blennorrhea and the relationship of the antigens to the developmental cycle. J. gen. Microbiol. 27, 501.CrossRefGoogle Scholar
Ross, M. R. & Gogolak, F. M. (1957). The antigenic structure of psittacosis and feline pneumonitis viruses. I. Isolation of complement-fixing antigens with group and species specificity. Virology, 3, 343.CrossRefGoogle ScholarPubMed
Terzin, A. L., Fornazarić, M. R. & Birtašević, B. V. (1963). Demonstration of complement-fixation titres against the species-specific trachoma antigen in sera of trachoma patients. J. Hyg., Camb., 61, 31.CrossRefGoogle Scholar
Terzin, A. L., Matuka, S., Fornazarić, M. R. & Hlača, D. M. (1961). Preparation of group-specific Bedsonia antigens for use in complement-fixation reactions. Acta Virol. 5, 78.Google Scholar
Thygeson, P. (1960). Trachoma manual and atlas. Washington, Publ. Health Serv. Publ. No. 541.Google Scholar
Thygeson, P. (1962). Trachoma virus: historical background and review of isolates. In The Biology of the Trachoma Agent. Ann. N.Y. Acad. Sci. 98, 6.CrossRefGoogle Scholar
Weiss, E. & Dressler, H. R. (1962). Investigation of the stability of the trachoma agent. In The Biology of the Trachoma Agent. Ann. N.Y. Acad. Sci. 98, 250.CrossRefGoogle Scholar
Woolridge, R. L., Jackson, E. B. & Grayston, J. T. (1960). Serological relationship of trachoma, psittacosis and lymphogranuloma venereum viruses. Proc. Soc. exp. Biol., N. V., 104, 298.CrossRefGoogle ScholarPubMed