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The serological diagnosis of whooping cough

Published online by Cambridge University Press:  15 May 2009

Margaret E. macaulay
Margaret E. macaulay
Affiliation:
Public Health Laboratory, Withington Hospital, Manchester M20 8LR
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Summary

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Indirect haemagglutination (IHA), agglutination and complement fixation tests (CFT) for Bordetella pertussis antibodies were compared on paired sera from 52 suspected cases of whooping cough and single sera from 83 children with no recent history of whooping cough. All three tests detected serotype antibodies 1, 2 and 3, but the IHA test was the most sensitive; in seven cases it was the only test to show a rise in titre. It is recommended, particularly with vaccinated children, that the serological diagnosis of whooping cough should be based upon a rise in titre. There should be a gap of at least 2–4 weeks between serum samples, depending on the age and vaccination state of the child. The CFT appears to detect a different antibody from that detected by the other two tests, and in three cases it was the only test to show a rise in titre.

Type
Research Article
Information
Journal of Hygiene , Volume 83 , Issue 1 , August 1979 , pp. 95 - 102
Copyright
Copyright © Cambridge University Press 1979

References

Adonajlo, A. & Kozerska, D. (1970). The value of the passive haemagglutination test in the seroepidemiology of pertussis. Epidemiological Review 24, 333–41.Google ScholarPubMed
Andersen, E. K. (1953). Serological studies on H. pertussis, H. parapertussis and H. bronchisepticus. Acta pathologica et microbiologica scandinavica 33, 202–24.CrossRefGoogle Scholar
Bradstreet, C. M. P., Tannahill, A. J., Edwards, J. M. B. & Benson, P. F. (1972). Detection of Bordetella pertussis antibodies in human sera by complement-fixation and immunofluorescence. Journal of Hygiene 70, 75–83.CrossRefGoogle ScholarPubMed
Combined scottish study (1970). Diagnosis of whooping cough: comparison of serological tests with isolation of Bordetella pertussis. British Medical Journal iv, 637–9.Google Scholar
Holt, L. B. & SpasojevÍc, V. (1968). The role of surface antigens in the protective potency of Bordetella pertussis suspensions as measured by the intracerebral challenge technique in mice. Journal of Medical Microbiology 1, 119–26.CrossRefGoogle ScholarPubMed
Linnemann, C. C., Bass, J. W. & Smith, M. H. D. (1968). The carrier state in pertussis. American Journal of Epidemiology 88, 422–7.CrossRefGoogle ScholarPubMed
Preston, N. W. (1970). Technical problems in the laboratory diagnosis and prevention of whooping-cough. Laboratory Practice 19, 482–6.Google ScholarPubMed
Preston, N. W. & Stanbridge, T. N. (1972). Efficacy of pertussis vaccine: a brighter horizon. British Medical Journal iii, 448–51.CrossRefGoogle Scholar
Schubert, J. H., Eleff, M. G. & Hermann, G. J. (1961). Haemagglutination test for pertussis antibody with a soluble extract of Bordetella pertussis. American Journal of Public Health and the Nation's Health 51, 441–5.CrossRefGoogle ScholarPubMed
Sequeira, P. J. L. & Eldridge, A. E. (1973). Treponemal haemagglutination test. British Journal of Venereal Diseases 49, 242–8.Google ScholarPubMed
Soare, I., Barber, C., Dumitresco, M. & Scurtu, A. (1964). Sur l'hémagglutination passive (indirecte) dans le diagnostic de la coqueluche; corrélation avec la réaction d'agglutination. Archives Roumaines de Pathologie expérimentale et de Microbiologie 23, 35–44.Google Scholar
Thomas, G. (1975). Respiratory and humoral immune response to aerosol and intramuscular pertussis vaccine. Journal of Hygiene 74, 233–7.CrossRefGoogle ScholarPubMed