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Serological characterization of group-A streptococci associated with skin sepsis in meat handlers

Published online by Cambridge University Press:  15 May 2009

Cherry A. M. Fraser ,
Lyn C. Ball ,
C. A. Morris  and
N. D. Noah
Cherry A. M. Fraser
Affiliation:
Streptococcus Reference Laboratory, Central Public Health Laboratory, Colindale Avenue, London NW 9 5 HT
Lyn C. Ball
Affiliation:
Streptococcus Reference Laboratory, Central Public Health Laboratory, Colindale Avenue, London NW 9 5 HT
C. A. Morris
Affiliation:
Public Health Laboratory, Shrewsbury SY 3 8 XH
N. D. Noah
Affiliation:
Epidemiological Research Laboratory, Central Public Health Laboratory, Colindale Avenue, London NW 9 5 HT
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A series of outbreaks of skin sepsis among meat handlers in England during 1974 and 1975 afforded an opportunity to study the group-A streptococci commonly isolated from the lesions. Few of these streptococci could be M typed with existing antisera. Intensive study of strains from two outbreaks of sepsis in one abattoir in Shropshire led to the recognition of three new provisional M types. The streptococci were first sorted according to T-typing pattern and ability to produce opacity factor. Opacity-factor producing strains with the same T pattern were then screened for inhibition of opacity production by the sera of convalescents from the same outbreak. Finally, M antisera were made in rabbits against representative cultures.

Sera against the three new provisional types were used to re-examine streptococci from 20 other outbreaks or incidents of sporadic infection among meat handlers. This increased the proportion of typable strains from 3% to 55%. Two of the new provisional types (nos. 2015 and 1658; both T25/Imp 19, opacity-factor positive) were confined to the Shropshire outbreak, but the third (no. 2681; T14, opacity-factor negative) was found among strains from meat workers in eight other geographically distinct areas. In all, 31% of 131 distinct strains from meat workers, but less than 1% of 2816 strains from other British sources, belonged to provisional type 2681. Thus, in Britain, one M-type of group-A streptococcus appears at present to be almost exclusively associated with sepsis in meat workers.

Type
Research Article
Information
Journal of Hygiene , Volume 78 , Issue 2 , April 1977 , pp. 283 - 296
Copyright
Copyright © Cambridge University Press 1977

References

REFERENCES

Ginsberg, I. (1972). Mechanisms of cell and tissue injury induced by group A streptococci: relation to post streptococcal sequelae (review). Journal of Infectious Diseases 126, 294–340.CrossRefGoogle Scholar
Glezen, W.P., de Walt, J. L., Lindsay, R. L. & Dillon, H. C. Jr (1972). Epidemic pyoderma caused by nephritogenic streptococci in college athletes. Lancet i, 301–4.CrossRefGoogle Scholar
Hook, E. W., Wagner, R. R. & Lancefield, R. C. (1960). An epizootic in Swiss mice caused by group A streptococcus, newly designated type 50. American Journal of Hygiene 72, 111–19.Google ScholarPubMed
Maxted, W. R., Widdowson, J. P. & Fraser, C. A. M. (1973 a). Antibody to streptococcal opacity factor in human sera. Journal of Hygiene 71, 35–42.CrossRefGoogle ScholarPubMed
Maxted, W. R., Widdowson, J. P., Fraser, C. A. M., Ball, L. C. & Bassett, D. C. J. (1973 b). The use of the serum opacity reaction in the typing of group A streptococci. Journal of Medical Microbiology 6, 83–90.CrossRefGoogle ScholarPubMed
Parker, M. T., Tomlinson, A. J. H. & Williams, R. E. O. (1955). Impetigo contagiosa: the association of certain types of Staphylococcus aureus and Streptococcus pyogenes with superficial skin infections. Journal of Hygiene 53, 458–73.Google ScholarPubMed
Public Health Laboratory Service, (1975 a). Skin infections in meat handlers. British Medical Journal i, 526.Google Scholar
Public Health Laboratory Service, (1975 b). Skin infections in meat handlers. British Medical Journal i, 583.Google Scholar
Rotta, J., Krause, R. M., Lancefield, R. C., Everly, W. & Lackland, H. (1971). New approaches for the laboratory recognition of M types of group A streptococci. Journal of Experimental Medicine 134, 1298–315.CrossRefGoogle ScholarPubMed
Wannamaker, L. W. (1970). Differences between streptococcal infections of the throat and of the skin. New England Journal of Medicine 282, 23–31, 78–85.CrossRefGoogle ScholarPubMed
Widdowson, J. P., Maxted, W. R. & Grant, D. L. (1970). The production of opacity in serum by group A streptococci and its relationship with the presence of M antigen. Journal of General Microbiology 61, 343–53.CrossRefGoogle Scholar
Williams, R. E. O. (1958). Laboratory diagnosis of streptococcal infection. Bulletin of the World Health Organization 19, 153–76.Google Scholar
Williams, R. E. O. & Maxted, W. R. (1953). The type classification of Streptococcus pyogenes. Atti del VI Congresso Internazionale di Microbiologia Roma, 6–12 Settembre 1953, Vol. 1, Sez. 1, pp. 46–9.Google Scholar