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Sensitivity of bluetongue virus to lipid solvents, trypsin and pH changes and its serological relationship to arboviruses*

Published online by Cambridge University Press:  15 May 2009

S.-E. Svehag ,
Louise Leendertsen  and
J. R. Gorham
S.-E. Svehag
Affiliation:
Department of Veterinary Science, Washington State University Pullman, Washington
Louise Leendertsen
Affiliation:
The Animal Disease Research Station, Animal Disease and Parasite Research Division, U.S. Department of Agriculture, Pullman, Washington
J. R. Gorham
Affiliation:
The Animal Disease Research Station, Animal Disease and Parasite Research Division, U.S. Department of Agriculture, Pullman, Washington
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Bluetongue virus was found to be resistant to ether, chloroform and sodium deoxycholate under a variety of conditions but sensitive to treatment with trypsin. The virus had a narrow zone of pH stability between pH 6 and 8 in Michaelis buffer. Below pH 6 bluetongue was irreversibly inactivated within 1 min. at 37° C. In many of its characteristics, bluetongue virus appears to be closely related to the reoviruses.

Type
Research Article
Information
Journal of Hygiene , Volume 64 , Issue 3 , September 1966 , pp. 339 - 346
Copyright
Copyright © Cambridge University Press 1966

References

REFERENCES

Andrews, C. H. (1964). Viruses of Vertebrates. London: Baillière, Tindall and Cox.Google Scholar
Casals, J. (1961). Procedures for identification of arthropod-borne viruses. Bull. Wld Hlth Org. 24, 723.Google ScholarPubMed
Cheng, P-Y. (1958). The inactivation of group B arthropod-borne animal viruses by proteases. Virology 6, 129.CrossRefGoogle Scholar
Clarke, D. H. (1960). Antigenic analysis of certain group B arthropod-borne viruses by antibody absorption. J. exp. Med. 111, 21.CrossRefGoogle Scholar
Du Toit, R. M. (1944). The transmission of blue-tongue and horse sickness by Culicoides. Onderstepoort. J. vet. Sci. Anim. Ind. 19, 7.Google Scholar
Foster, N. M., Jones, R. H. & McCrory, B. R. (1963). Preliminary investigations on insect transmission of bluetongue virus in sheep. Am. J. vet. Res. 24, 1195.Google ScholarPubMed
Gard, S. (1957). Immuno-inactivation of poliovirus. Arch. ges. Virusforsch. 7, 449.CrossRefGoogle ScholarPubMed
Gomatos, P. J. & Tamm, I. (1962). Reactive sites of reovirus type 3 and their interaction with receptor substances. Virology 17, 455.CrossRefGoogle ScholarPubMed
Gomatos, P. J., Tamm, I.Dales, S. & Franklin, R. M. (1962). Reovirus type 3: physical characteristics and interaction with L cells. Virology 17, 441.CrossRefGoogle ScholarPubMed
Gorham, J. R. (1957). A simple technique for the inoculation of the chorio-allantoic membrane of chicken embryos. Am. J. vet. Res. 18, 691.Google Scholar
Green, N. M. (1953). Competition among trypsin inhibitors. J. biol. Chem. 205, 535.CrossRefGoogle ScholarPubMed
Gresser, I. & Enders, J. F. (1961). The effect of trypsin on representative myxoviruses. Virology 13, 420.CrossRefGoogle ScholarPubMed
Jordan, L. E. & Mayor, H. D. (1962). The fine structure of reovirus, a new member of the icosahedral series. Virology 17, 597.CrossRefGoogle Scholar
Kunitz, M. (1947). Crystalline soybean trypsin inhibitor. II. General properties. J. gen. Physiol. 30, 291.CrossRefGoogle ScholarPubMed
Livingstone, C. W. & Moore, R. W. (1962). Cytochemical changes of bluetongue virus in tissue cultures. Am. J. vet. Res. 23, 701.Google Scholar
Mayor, H. D. & Jordan, L. E. (1965). Studies on reovirus. I. Morphologic observations on the development of reovirus in tissue culture. Expl molec. Path. 4, 40.CrossRefGoogle ScholarPubMed
McKercher, D. G., McGowan, B. & Saito, J. K. (1954). Studies on bluetongue. I. Isolation, identification and typing of the bluetongue virus and a preliminary report on the serodiagnosis of the disease. Proc. Am. vet. med. Ass. p. 167.Google Scholar
Merrill, M. H. (1936). Effect of purified enzymes on viruses and Gram-negative bacteria. J. exp. Med. 64, 19.CrossRefGoogle ScholarPubMed
Morgan, C., Howe, C. & Rose, H. M. (1961). Structure and development of viruses as observed in the electron microscope. V. Western equine encephalomyelitis virus. J. exp. Med. 113, 219.CrossRefGoogle ScholarPubMed
Mussgay, M. & Rott, R. (1964). Studies on the structure of a hemagglutinating component of a group A arbo virus (Sindbis). Virology 23, 573.CrossRefGoogle Scholar
Polson, A. & Deeks, D. (1963). Electron microscopy of neurotropic African horse-sickness virus. J. Hyg., Camb., 61, 149.CrossRefGoogle ScholarPubMed
Price, D. A. & Hardy, W. T. (1954). Isolation of the bluetongue virus from Texas sheep—Culicoides shown to be a vector. J. Am. vet. med. Ass. 124, 255.Google ScholarPubMed
Studdert, M. J. (1965). Sensitivity of bluetongue virus to ether and sodium deoxycholate. Proc. Soc. exp. Biol. Med. 118, 1006.CrossRefGoogle ScholarPubMed
Svehag, S-E. (1962). Quantitative studies of blue tongue virus in mice. Arch. ges. Virusforsch. 12, 363.CrossRefGoogle Scholar
Svehag, S-E. (1963). Thermal inactivation of blue tongue virus. Arch. ges. Virusforsch. 13, 499.CrossRefGoogle ScholarPubMed
Vasquez, C. & Tournier, P. (1962). The morphology of reovirus. Virology 17, 503.CrossRefGoogle ScholarPubMed
Wallis, C., Smith, K. O. & Melnick, J. L. (1964). Reovirus activation by heating and inactivation by cooling in MgCl2 solutions. Virology 22, 608.CrossRefGoogle Scholar
Wecker, E. (1959). The extraction of infectious virus nucleic acid with hot phenol. Virology 7, 241.CrossRefGoogle ScholarPubMed