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Salmonellas in Danish pigs: a comparison of three isolation methods

Published online by Cambridge University Press:  19 October 2009

Niels Skovgaard ,
Sven Gade Christensen  and
A. W. Gulistani
Niels Skovgaard
Affiliation:
Institute of Hygiene and Microbiology, Royal Veterinary and Agricultural University, Copenhagen, Denmark
Sven Gade Christensen
Affiliation:
Institute of Hygiene and Microbiology, Royal Veterinary and Agricultural University, Copenhagen, Denmark
A. W. Gulistani
Affiliation:
Faculty of Veterinary Science, University of Kabul, Kabul, Afghanistan
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Summary

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Caecal samples from 350 Danish bacon pigs were investigated for salmonella using three methods of isolation. (1) Direct inoculation of 1 g of faeces into 10 ml of Muller–Kaufmann medium (MK medium) with addition of 0·3% Teepol 610 and subculture on Brilliant Green lactose sucrose phenol-red agar (BLSF agar) with 0·3% Teepol G10. (2) Pre-enrichment of 5 g of faeces into buffered peptone water with addition of 1% Teepol 610 followed by enrichment of 1 ml in 10 ml MK medium with 1% Teepol 610 and subculture on BLSF agar with 0·3% Teepol. (3) Incubation of 0·1 ml of the pre-enrichment (2) into 10 ml Rappaport-Vassiliadis medium (RV 10 medium) incubated at 43°C, subculture on BLSF agar.

The MK media with and without pre-enrichment yielded higher findings than the RV 10 media. In total, 28 (8%) of the pigs were found positive, representing 11 (7·4%) of a total of 142 herds investigated. Lymph glands were collected at a later date from six of the positive herds. Five of the herds were found positive.

The number of salmonellas in the glands was low, probably less than ten per gram.

Type
Research Article
Information
Journal of Hygiene , Volume 95 , Issue 1 , August 1985 , pp. 69 - 75
Copyright
Copyright © Cambridge University Press 1985

References

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