Hostname: page-component-586b7cd67f-t8hqh Total loading time: 0 Render date: 2024-11-25T05:50:02.213Z Has data issue: false hasContentIssue false

Rubella-specific IgG subclass concentrations in sera using an enzyme-linked immunosorbent assay (ELISA): the effect of different sources of rubella antigen

Published online by Cambridge University Press:  15 May 2009

H. I. J Thomas
Affiliation:
Department of Virology, Preston Infirmary, Preston PR1 6PS
P. Morgan-Capner
Affiliation:
Department of Virology, Preston Infirmary, Preston PR1 6PS
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Five rubella antigens were evaluated in an antiglobulin enzyme-linked immunosorbent assay for rubella-specific IgG subclass antibody. One monoclonal anti-human IgG subclass antibody was used for each of IgG1, IgG2 and IgG4, but two were compared for IgG3. A total of 101 sera were tested from cases of rubella in the distant past and from cases of primary rubella, reinfection and following immunization. Only one serum gave a discrepant result for specific IgG1, being positive with only one rubella antigen, a commercially prepared antigen coated on to microtitre wells (Enzygnost; Behringwerke). No sera contained detectable specific IgG2. Only four sera contained specific IgG4, and this was detectable only with Enzygnost antigen. For specific IgG3 little difference was observed between the two monoclonal anti-human IgG3 subclass antibodies; only two very weakly positive sera gave discrepant results. However, varying results were obtained for specific IgG3 with the different antigens. Enzygnost gave more positive results for specific IgG3 with most categories of sera.

It is concluded that the differences between various reports of the rubella-specific IgG subclass profile cannot be explained entirely by the use of different rubella antigens.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1988

References

REFERENCES

Beck, O. E. (1981). Distribution of virus antibody activity among human IgG subclasses. Clinical and Experimental Immunology 43, 626632.Google ScholarPubMed
Doerr, H. W., Fleischer, G. & Wiesman, M. (1984). Detection of rubella specific antibodies in different immunoglobulin-(sub)classes by ELISA. Abstract. Thirty-ninth meeting of the Deutsche Gesellschaft, Hygiene and Microbiology.Google Scholar
Kurtz, J. B., Mortimer, P. P., Mortimer, P. R., Morgan-Capner, P., Shafi, M. S. & White, G. B. B. (1980). Rubella antibody measured by radial haemolysis. Characterization and performance of a simple screening method for use in diagnostic laboratories. Journal of Hygiene 84, 213222.CrossRefGoogle Scholar
Lehtinen, M. (1987). Affinity and subclass distribution of IgG and subclass antibodies following vaccination with a live rubella vaccine. Vaccine 5, 8889.CrossRefGoogle Scholar
Linde, G. A. (1985). Subclass distribution of rubella virus-specific immunoglobulin G. Journal of Clinical Microbiology 21, 117121.CrossRefGoogle ScholarPubMed
Sarnesto, A., Ranta, S., Vaanarea, P. & Makela, O. (1985). Proportions of IgG classes and subclasses in rubella antibodies. Scandinavian Journal of Immunology 2, 275282.CrossRefGoogle Scholar
Skvaril, F. (1983) Human LgG subclasses in anti-viral antibodies determined with monoclonal antibodies in ELISA. Immunoenzymatic Techinques (ed. Avrameas, S.), pp. 287290. Am-sterdam: Elsevier.Google Scholar
Skvaril, F. & Schilt, U. (1984). Characterization of subclasses and light chain types of IgG antibodies to rubella. Clinical and Experimental Immunology 55, 672676.Google ScholarPubMed
Stokes, A., Mims, C. A. & Grahame, R. (1986). Subclass distribution of IgG and IgA responses to rubella virus in man. Journal of Medical Microbiology 21, 283285.CrossRefGoogle ScholarPubMed
Thomas, H. I. J. & Morgan-Capner, P. (1988). Specific IgG subclass antibody in rubella virus infections. Epidemiology and Infection 100, 443454.CrossRefGoogle ScholarPubMed