Hostname: page-component-78c5997874-j824f Total loading time: 0 Render date: 2024-11-06T01:19:12.830Z Has data issue: false hasContentIssue false
  • English
  • Français

Ribotyping of Staphylococcus aureus: an assessment using well–defined strains

Published online by Cambridge University Press:  15 May 2009

J. F. Richardson ,
P. Aparicio ,
R. R. Marples  and
B. D. Cookson
J. F. Richardson
Affiliation:
Laboratory of Hospital Infection, Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT, UK
P. Aparicio
Affiliation:
Laboratory of Hospital Infection, Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT, UK
R. R. Marples
Affiliation:
Laboratory of Hospital Infection, Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT, UK
B. D. Cookson
Affiliation:
Laboratory of Hospital Infection, Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT, UK
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Ribotyping, with homologous or heterologous (Escherichia coli) r–RNA, of the propagating strains for phages of the international set for strains of Staphylococcus aureus of human origin was undertaken to determine the discrimination of this typing method. Ribotyping could distinguish between strains of different phage groups, but could not distinguish between seven phage group III strains of different phage type. Ribotyping may be a useful adjunct to phage typing in S. aureus but is unlikely to replace it as the primary method of epidemiological typing.

Type
Research Article
Information
Epidemiology & Infection , Volume 112 , Issue 1 , February 1994 , pp. 93 - 101
Copyright
Copyright © Cambridge University Press 1994

References

REFERENCES

Fisk, RT. Studies on staphylococci II. Identification of Staphylococcus aureus strains by means of bacteriophage. J Infect Dis 1942; 71: 161–5.CrossRefGoogle Scholar
Blair, JE, Williams, REO. Phage typing of the staphylococci. Bull WHO 1961; 24: 771–84.Google ScholarPubMed
Marples, RR, Richardson, JF, Kerr, GE, Kerr, S, Mackintosh, CA. Methicillin-resistent Staphylococcus aureus (MRSA) in England and Wales. Zbl Bakt Suppl 1991; 21: 427–30.Google Scholar
Monzon-Moreno, C, Aubert, S, Morven, A, el Solh, N. Usefulness of three probes in typing isolates of methicillin-resistant Staphylococcus aureus (MRSA). J Med Microbiol 1991; 35: 80–8.CrossRefGoogle ScholarPubMed
Kerr, S, Kerr, GE, Mackintosh, CA, Marples, RR. A survey of methicillin-resistant Staphylococcus aureus In England and Wales. J Hosp Infect 1990; 16: 35–48.CrossRefGoogle ScholarPubMed
Townsend, DE, Ashdown, N, Bolton, S et al. , The international spread of methicillin-resistant Staphylococcus aureus. J Hosp Infect 1987; 9: 60–71.CrossRefGoogle ScholarPubMed
Gaston, MA, Duff, PS, Naidoo, J et al. , Evaluation of electrophoretic methods for typing methicillin-resistant. Staphylococcus aureus. J Med Microbiol; 26: 189–99.CrossRefGoogle Scholar
Mulvey, M, Arbuthnott, JP, Coleman, D. Molecular typing of methicillin and gentamicin-resistant Staphylococcus aureus in Dublin. Eur J Clin Microbiol Infect Dis 1986; 5: 719–25.Google ScholarPubMed
Hadorn, K, Lenz, W, Kayser, FH, Shalit, I, Krasemann, C. Use of a ribosomal RNA gene probe for the epidemiological study of methicillin and ciprofloxacin resistant Staphylococcus aureus. Eur J Clin Microbiol Infect Dis 1990; 9: 649–53.CrossRefGoogle ScholarPubMed
Preheim, L, Pitcher, D, Owen, R, Cookson, B. Typing of methicillin resistant and susceptible Staphylococcus aureus strains by ribosomal RNA gene restriction patterns using a biotinylated probe. Eur J Clin Microbiol Infect Dis 1991; 10: 428–36.CrossRefGoogle ScholarPubMed
Parker, MT. The significance of phage-typing patterns in Staphylococcus aureus. In: Staphylococci and staphylococcal infections, vol. I, Easmon, CSF, Adlam, C, eds. London: Academic Press, 1983.Google Scholar
Ralston, DJ, Baer, BS. Host-controlled changes of staphylococcal phage 3C affecting its broad group typing pattern. J Gen Microbiol 1964; 36: 25–36.CrossRefGoogle ScholarPubMed
Asheshov, EH, Coe, AW, Porthouse, A. Properties of strains of Staphylococcus aureus of the 94/96 complex. J Med Microbiol 1974; 10: 171–8.CrossRefGoogle Scholar
van Boven, CP, Stobberingh, EE, Verhoef, J, Winkler, KC. Restriction and modification of phages in staphylococcal phage typing. Ann NY Acad Sci 1974; 236: 376–88.CrossRefGoogle ScholarPubMed
Owen, RJ. Chromosomal DNA fingerprinting – a new method of species and strain identification applicable to microbial pathogens. J Med Microbiol 1989; 30: 89–99.CrossRefGoogle ScholarPubMed
Parker, MT, Jevons, MP. A survey of methicillin resistance in Staphylococcus aureus. Postgrad Med J 1964; 40 Suppl: 170–8.CrossRefGoogle ScholarPubMed
Wilson, GS, Atkinson, JD. Typing of staphylococci by the bacteriophage method. Lancet 1945; i: 6352–3.Google Scholar
Williams, REO, Rippon, JE. Bacteriophage typing of Staphylococcus aureus. J Hyg 1952; 50: 320–53.CrossRefGoogle ScholarPubMed
Korman, RZ, Bearman, DT. Genetic transduction with staphylophage. J Bacteriol 1962; 84: 228–36.CrossRefGoogle ScholarPubMed
Jevons, MP, Parker, MT. The evolution of a new hospital strain of Staphylococcus aureus. J Clin Pathol 1964; 17: 243–50.CrossRefGoogle ScholarPubMed
Blouse, LE, Stringfield, WB, Marraro, RV et al. , Activity and characteristics of a new staphylococcal phage 94. Proc Soc Exp Biol Med 1973; 142: 572–6.CrossRefGoogle ScholarPubMed
Jordens, JZ, Hall, LMC. Characterization of methicillin-resistant Staphylococcus aureus isolates by restriction endonuclease digestion of chromosomal DNA. J Med Microbiol 1988; 27: 117–23.CrossRefGoogle ScholarPubMed
Richardson, JF, Quoraishi, AHM, Francis, BJ, Marples, RR. Beta-lactamase negative, methicillin-resistant Staphylococcus aureus in a newborn nursery: report of an outbreak and laboratory investigations. J Hosp Infect 1990; 16: 109–21.CrossRefGoogle Scholar
Pitcher, DG, Saunders, NA, Owen, RJ. Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett Appl Microbiol 1989; 8: 151–6.CrossRefGoogle Scholar
Richardson, JF, Noble, WC, Marples, RR. Identification and epidemiological typing of the staphylococci. In: Identification methods in applied and environmental microbiology, Skinner, FA, Jones, D, eds. Society for Applied Bacteriology, Technical Series 29. 1992.Google Scholar
Maniatis, T, Fitsch, EF, Sambrook, J. Molecular cloning: a laboratory manual. Cold Spring Harbor: Cold Spring Harbor Press, 1982.Google Scholar
Southern, E. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 1975; 98: 503–17.CrossRefGoogle ScholarPubMed
Pitcher, DG, Owen, RJ, Dyal, P, Beck, A. Synthesis of a biotinylated DNA probe to detect ribosomal DNA cistrons in Providencia stuartii. FEMS Microbiol Lett 1987; 48: 283–7.CrossRefGoogle Scholar
Dice, LR. Measures of the amount of the ecologic association between species. Ecology 1945; 26: 297–302.CrossRefGoogle Scholar
Jones, D, Sackin, MJ. Numerical methods in the classification and identification of bacteria with especial reference to the Enterobacteriacae. In: Microbiological classification and identification, Goodfellow, M.Board, RG, eds. Society for Applied Bacteriology, Symposium Series 8. London: Academic Press, 1980.Google Scholar
Rountree, PM, Freeman, BM. Infections caused by a particular phage type of Staphylococcus aureus. Med J Aust 1955; ii: 157–67.CrossRefGoogle Scholar
Aparicio, P, Richardson, J, Martin, S, Vindel, A, Marples, RR, Cookson, BD. An epidemic methicillin-resistant strain of Staphylococcus aureus in Spain. Epidemiol Infect 1992; 108: 287–98.CrossRefGoogle ScholarPubMed
Richardson, JF, Reith, S. Characterization of a strain and methicillin-resistant Staphylococcus aureus (EMRSA-15) by conventional and molecular methods. J Hosp Infect 1993; 24F3F3: in press.Google Scholar