Hostname: page-component-cd9895bd7-7cvxr Total loading time: 0 Render date: 2024-12-22T15:52:47.085Z Has data issue: false hasContentIssue false
  • English
  • Français

Ribotyping as an epidemiologic tool for Escherichia coli

Published online by Cambridge University Press:  15 May 2009

E. Tarkka ,
H. Åhman  and
A. Siitonen
E. Tarkka
Affiliation:
Laboratory of Enteric Pathogens, National Public Health Institute, Helsinki, Finland
H. Åhman
Affiliation:
Laboratory of Enteric Pathogens, National Public Health Institute, Helsinki, Finland
A. Siitonen*
Affiliation:
Laboratory of Enteric Pathogens, National Public Health Institute, Helsinki, Finland
*
*Anja Siitonen, National Public Health Institute, Mannerheimintie 166, FIN-00300 Helsinki, Finland.
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Restriction fragment length polymorphism of ribosomal RNA genes was analysed among 133 Escherichia coli strains predominantly from blood and urine, including 21 isolates from faeces of healthy persons. The strains had also been characterized for their O:K:H serotypes, for the presence of P, S and type 1C fimbriae, non–P, non-S mannose–resistant haemagglutinins and haemolysin production. Hind III–digested genomic DNA was subjected to Southern blot analysis with either plasmid pKK3535 containing E. coli rRNA operon or purified rRNA as a probe. Among the 133 strains 20 ribotypes were obtained. The distribution of strains into different ribotypes generally correlated with their O:K:H serotype. Ribotype variation within serotypes was mainly seen among strains with the K5 capsule. The origin of the strains or the presence of virulence-associated factors did not correlate with the ribotype. In conclusion, ribotyping appears to be a valuable method in epidemiologic studies especially when the serotyping methods are not available.

Type
Research Article
Information
Epidemiology & Infection , Volume 112 , Issue 2 , April 1994 , pp. 263 - 274
Copyright
Copyright © Cambridge University Press 1994

References

REFERENCES

Ørskov, F, Ørskov, I. Summary of a workshop on the clone concept in the epidemiology, taxonomy, and evaluation of the Enterobacteriaceae and other bacteria. J Infect Dis 1983; 148: 346–57.CrossRefGoogle Scholar
Johnson, JR. Virulence factors in Escherichia coli urinary tract infection. Clin Microbiol Rev 1991; 4: 80–128.CrossRefGoogle ScholarPubMed
Korhonen, TK, Valtonen, MV, Parkkinen, J et al. , Serotypes, hemolysin production, and receptor recognition of Escherichia coli strains associated with neonatal sepsis and meningitis. Infect Immun 1985; 48: 486–91.CrossRefGoogle ScholarPubMed
Siitonen, A, Takala, A, Ratiner, YA, Pere, A, Mäkelä, PH. Invasive Escherichia coli infections in children: bacterial characteristics in different age groups and clinical entities. Pediatr Infect Dis J 1993; 12: 606–12.CrossRefGoogle ScholarPubMed
Tullus, K, Brauner, A, Fryklund, B et al. , Host factors versus virulence-associated bacterial characteristics in neonatal and infantile bacteraemia and meningitis caused by Escherichia coli. J Med Microbiol 1992; 36: 203–8.CrossRefGoogle ScholarPubMed
Achtman, M, Pluschke, G. Clonal analysis of descent and virulence among selected Escherichia coli. Ann Rev Microbiol 1986; 40: 185–210.CrossRefGoogle ScholarPubMed
Väisänen-Rhen, V, Elo, J, Väisänen, E et al. , P–fimbriated clones among uropathogenic Escherichia coli strains. Infect Immun 1984; 43: 149–55.CrossRefGoogle ScholarPubMed
Arbeit, RD, Arthur, M, Dunn, R, Kim, C, Selander, RK, Goldstein, R. Resolution of recent evolutionary divergence among Escherichia coli from related lineages: the application of pulsed field electrophoresis to molecular epidemiology. J Infect Dis 1990; 161: 230–5.CrossRefGoogle ScholarPubMed
Ott, M, Bender, L, Blum, G et al. , Virulence patterns and long–range genetic mapping of extraintestinal Escherichia coli K1, K5 and K100 isolates: Use of pulsed–field gel electrophoresis. Infect Immun 1991; 59: 2664–72.CrossRefGoogle ScholarPubMed
Arthur, M, Arbeit, RD, Kim, C et al. , Restriction fragment length polymorphism among uropathogenic Escherichia coli isolates: pap–related sequences compared with rrn operons. Infect Immun 1990; 58: 471–9.CrossRefGoogle ScholarPubMed
Grimont, F, Grimont, PAD. Ribosomal nucleic acid gene restriction patterns as potential taxonomic tools. Ann Inst Pasteur/Microbiol. 1986; 137B: 165–75.CrossRefGoogle Scholar
Stull, TL, LiPuma, JJ, Edling, TD. A broad–spectrum probe for molecular epidemiology of bacteria: ribosomal RNA. J Infect Dis 1988; 157: 280–6.CrossRefGoogle ScholarPubMed
Bingen, EH, Denamur, E, Picard, P et al. , Molecular epidemiology unravels the complexity of neonatal Escherichia coli acquisition in twins. J Clin Microbiol 1992; 30: 1896–8.CrossRefGoogle ScholarPubMed
Bingen, E, Cave, H, Aujard, Y et al. , Molecular analysis of multiply recurrent meningitis due to Escherichia coli K1 in an infant. Clin Infect Dis 1993; 16: 82–5.CrossRefGoogle ScholarPubMed
Ikäheimo, R, Siitonen, A, Kärkkäinen, U, Mäkelä, PH. Virulence characteristics of Escherichia coli in nosocomial urinary tract infection. Clin Infect Dis 1993; 16: 785–91.CrossRefGoogle ScholarPubMed
Ikäheimo, R, Siitonen, A, Kärkkäinen, U, Kuosmanen, P, Mäkelä, PH. Characteristics of Escherichia coli in acute community acquired cystitis of adult women. Scand J Infect Dis. In press.CrossRefGoogle Scholar
Siitonen, A, Escherichia coli in fecal flora of healthy adults: serotypes, P and type 1C fimbriae, non-P mannose-resistant adhesins, and hemolytic activity. J Infect Dis 1992; 166: 1058–65.CrossRefGoogle ScholarPubMed
Pitcher, DG, Saunders, NA, Owen, RJ. Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett Appl Microbiol 1989; 8: 151–6.CrossRefGoogle Scholar
Brosius, J, Ullrich, A, Raker, MA et al. , Construction and fine mapping of recombinant plasmids containing the rrnB ribosomal RNA operon of E. coli. Plasmid 1981; 6: 112–8.CrossRefGoogle ScholarPubMed
Gupta, DS, Jann, B, Schmidt, G et al. , Coliphage K5, specific for E. coli exhibiting the capsular K5 antigen. FEMS Microbiol Lett 1982; 14: 75–8.CrossRefGoogle Scholar
Macnab, RM. Flagella. In: Neidhardt, FC et al. , eds. Escherichia coli and Salmonella typhimurium. Cellular and molecular biology. Washington DC: American Society for Microbiology, 1987; 70–83.Google Scholar
Smith, JM, Dowson, CG, Spratt, BG. Localized sex in bacteria. Nature 1991; 349: 29–31.CrossRefGoogle ScholarPubMed
Plos, K, Hull, SI, Hull, RA et al. , Distribution of the P–associated–pilus (pap) region among Escherichia coli from natural sources: evidence for horizontal gene transfer. Infect Immun 1989; 57: 1604–11.CrossRefGoogle ScholarPubMed
Arthur, M, Johnson, CE, Rubin, RH et al. , Molecular epidemiology of adhesin and hemolysin virulence factors among uropathogenic Escherichia coli. Infect Immun 1989; 57: 303–13.CrossRefGoogle ScholarPubMed
Ott, M, Hacker, J, Schmoll, T, Jarchau, T, Korhonen, TK, Goebel, W. Analysis of the genetic determinants coding for the S fimbrial adhesin (sfa) in different Escherichia coli strains causing meningitis or urinary tract infection. Infect Immun 1986; 54: 646–53.CrossRefGoogle Scholar