Hostname: page-component-586b7cd67f-rcrh6 Total loading time: 0 Render date: 2024-11-26T01:22:18.772Z Has data issue: false hasContentIssue false
  • English
  • Français

Restriction fragment length polymorphisms among the flagellar genes of the Lior heat-labile serogroup reference strains and field strains of Campylobacter jejuni and C. coli

Published online by Cambridge University Press:  15 May 2009

A. P. Burnens ,
J. Wagner ,
H. Lior ,
J. Nicolet  and
J. Frey
A. P. Burnens
Affiliation:
National Reference Laboratory for Foodborne Diseases, University of Berne, Länggass-Str. 122, CH-3012 Berne, Switzerland
J. Wagner
Affiliation:
National Reference Laboratory for Foodborne Diseases, University of Berne, Länggass-Str. 122, CH-3012 Berne, Switzerland
H. Lior
Affiliation:
Laboratory Centre for Disease Control, Tunney's Pasture, Ottawa, Ontario K1A 0L2, Canada
J. Nicolet
Affiliation:
National Reference Laboratory for Foodborne Diseases, University of Berne, Länggass-Str. 122, CH-3012 Berne, Switzerland
J. Frey
Affiliation:
National Reference Laboratory for Foodborne Diseases, University of Berne, Länggass-Str. 122, CH-3012 Berne, Switzerland
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Several typing systems have been described for Campylobacter jejuni and C. coli, to assess the complex epidemiology of these important enteric pathogens. In the present study two typing methods, slide agglutination according to the Lior scheme, and the demonstration of restriction-fragment length polymorphisms (RFLP) of flagellar genes, have been used in parallel on a set of 194 strains. This set comprised 118 sero-reference strains of C. jejuni and C. coli of the Lior scheme, as well as 76 clinical isolates. All isolates were serotyped and subjected to PCR for amplification of flagellar genes, and the PCR product was restricted with Alu I. Flagellar genes could be amplified in 152 strains. Among 85 seroreference strains, 74 different RFLP patterns were observed, and among 67 clinical isolates, there were 36 patterns. There was only limited correlation between flagellar RFLP and the Lior serogroup, and the variability of patterns in serogroups HL2 and HL4 were as marked as the variability between serogroups. Flagellar gene RFLP patterns are shown to be stable, highly discriminatory epidemiologic markers.

Type
Research Article
Information
Epidemiology & Infection , Volume 114 , Issue 3 , June 1995 , pp. 423 - 431
Copyright
Copyright © Cambridge University Press 1995

References

REFERENCES

1.Patton, CM, Wachsmuth, IK, Evins, GM et al. . Evaluation of 10 methods to distinguish epidemic-associated Campylobacter strains. J Clin Microbiol 1991: 29: 680–8.CrossRefGoogle ScholarPubMed
2.Lior, H, Woodward, DL, Edgar, JA, Laroche, LJ, Gill, P. Serotyping of Campylobacter jejuni by slide agglutination based on heat-labile antigenic factors. J Clin Microbiol 1982; 15: 761–8.CrossRefGoogle ScholarPubMed
3.Harris, LA, Logan, SM, Guerry, P, Trust, TJ. Antigenic variation of Campylobacter flagella. J Bacteriol 1987: 169: 5066–71.CrossRefGoogle ScholarPubMed
4.Alm, RA, Guerry, P, Power, ME, Lior, H, Trust, TJ. Analysis of the role of flagella in the heat-labile Lior serotyping scheme of thermophilic campylobacters by mutant allele exchange. J Clin Microbiol 1991; 29: 2438–45.CrossRefGoogle ScholarPubMed
5.Owen, RJ, Fayos, A, Hernandez, J, Lastovica, A. PCR-based restriction fragment length polymorphism analysis of DNA sequence diversity of flagellin genes of Campylobacter jejuni and allied species. Mol Cell Probes 1993; 7: 471–80.CrossRefGoogle ScholarPubMed
6.Nachamkin, I, Bohachick, K, Patton, CM. Flagellin gene typing of Campylobacter jejuni by restriction fragment length polymorphism analysis. J Clin Microbiol 1993; 31: 1531–6.CrossRefGoogle ScholarPubMed
7.Alm, RA, Guerry, P, Trust, TJ. Distribution and polymorphism of the flagellin genes from isolates of Campylobacter coli and Campylobacter jejuni. J Bacteriol 1993; 175: 3051–7.CrossRefGoogle ScholarPubMed
8.Kilger, G, Grimont, PAD. Differentiation of Salmonella phase-1 flagellar antigen types by restriction of the amplified fliC gene. J Clin Microbiol 1993; 31: 1108–10.CrossRefGoogle ScholarPubMed
9.Fischer, SH, Nachamkin, I. Common and variable domains of the flagellin gene, flaA, in Campylobacter jejuni. Mol Microbiol 1991; 5: 1151–8.CrossRefGoogle ScholarPubMed
10.Guerry, P, Logan, SM, Thornton, S, Trust, TJ. Genomic organization and expression of Campylobacter flagellin genes. J Bacteriol 1990; 172: 1853–60.CrossRefGoogle ScholarPubMed
11.Nuijten, PJ, van Asten, FJ, Gaastra, W, van der Zeijst, BA. Structural and functional analysis of two Campylobacter jejuni flagellin genes. J Biol Chem 1990; 265: 17798–804.CrossRefGoogle ScholarPubMed
12.Khawaja, R, Neote, K, Bingham, HL, Penner, JL, Chan, VL. Cloning and sequence analysis of the flagellin gene of Campylobacter jejuni TGH9011. Curr Microbiol 1992; 24: 213–21.CrossRefGoogle Scholar
13.Pitcher, DG, Saunders, NA, Owen, RJ. Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett Appl Microbiol 1989; 8: 151–6.CrossRefGoogle Scholar
14.Sambrook, J, Fritsch, EF, Maniatis, T. Molecular cloning. A laboratory manual. New York: Cold Spring Harbor Laboratory Press. 1989.Google Scholar
15.Blaser, MJ, Taylor, DN, Feldman, RA. Epidemiology of Campylobacter jejuni infections. Epidemiol Rev 1983; 5: 157–76.CrossRefGoogle ScholarPubMed
16.Penner, JL, Hennessy, JN, Congi, RV. Serotyping of Campylobacter jejuni and Campylobacter coli on the basis of thermostable antigens. Eur J Clin Microbiol Infect Dis 1983; 2: 378–83.Google ScholarPubMed
17.Ewing, WH. Antigenic schema for Salmonella. In: Edwards and Ewing's identification of Enterobacteriaceae. 4th edn.New York: Elsevier. 1986: 247–318.Google Scholar
18.Upholt, WB. Estimation of DNA sequence divergence from comparison of restriction endonuclease digests. Nucleic Acids Res 1977: 4: 1257–65.CrossRefGoogle ScholarPubMed
19.Owen, RJ, Fitzgerald, C, Sutherland, K, Borman, P. Flagellin gene polymorphism analysis of Campylobacter jejuni infecting man and other hosts and comparison with biotyping and somatic antigen serotyping. Epidemiol Infect 1994; 113: 221–34.CrossRefGoogle ScholarPubMed
20.Kapperud, G, Skjerve, E, Vik, L et al. . Epidemiological investigation of risk factors for carnpvlobacter colonization in Norwegian broiler flocks. Epidemiol Infect 1993; 111: 245–55.CrossRefGoogle ScholarPubMed
21.Patton, CM, Nicholson, MA, Ostroff, SM, Ries, AA, Wachsmuth, IK, Tauxe, RV. Common somatic-O and heat-labile serotypes among Campylobacter strains from sporadic infections in the United-States. J Clin Microbiol 1993; 31: 1525–30.CrossRefGoogle ScholarPubMed