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Restriction fragment length polymorphism of the pMJ101-like plasmid and ribotyping in the fish pathogen Vibrio ordalii

Published online by Cambridge University Press:  15 May 2009

K. Pedersen ,
S. Koblavi ,
T. Tiainen  and
P. A. D. Grimont
K. Pedersen
Affiliation:
Royal Veterinary and Agricultural University, Department of Veterinary Microbiology, Laboratory of Fish Diseases, Bülowsvej 13, DK-1870 Frederiksberg C, Denmark,
S. Koblavi
Affiliation:
Centre National de Typage Moléculaire Entérique, Unité des Entérobactéries, Unité INSERM 399, Institut Pasteur, 28 rue du Docteur Roux, F-75724 Paris Cedex 15, France
T. Tiainen
Affiliation:
Royal Veterinary and Agricultural University, Department of Veterinary Microbiology, Laboratory of Fish Diseases, Bülowsvej 13, DK-1870 Frederiksberg C, Denmark,
P. A. D. Grimont
Affiliation:
Centre National de Typage Moléculaire Entérique, Unité des Entérobactéries, Unité INSERM 399, Institut Pasteur, 28 rue du Docteur Roux, F-75724 Paris Cedex 15, France
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A total of 32 Vibrio ordalii strains were studied for their plasmid content and shown to carry a plasmid of approximately 32 kb. This plasmid was subsequently subjected to restriction fragment length polymorphism (RFLP) studies. Using Hind III, three different restriction patterns were identified while BamHI cleaved the plasmid into a single linear fragment. The results suggest that the 32 kb plasmid is highly conserved but that some variation in restriction pattern occurs. The same set of strains was subjected to ribotyping. Using Mlu I, six different restriction patterns were demonstrated. Strains from the USA and Canada shared profiles with strains from Australia and Japan. Strains from Australia generated a single pattern whereas strains from North America were subdivided into three patterns, and the Japanese strains fell into five patterns. The results suggest that ribotyping in combination with RFLP studies of the pMJ101-like plasmid may be useful in epidemiological studies of V. ordalii.

Type
Research Article
Information
Epidemiology & Infection , Volume 117 , Issue 2 , October 1996 , pp. 385 - 391
Copyright
Copyright © Cambridge University Press 1996

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