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Restriction enzyme analysis and ribotyping distinguish Bordetella avium and Bordetella hinzii isolates

Published online by Cambridge University Press:  01 February 2000

R. E. SACCO ,
K. B. REGISTER  and
G. E. NORDHOLM
R. E. SACCO
Affiliation:
Respiratory Diseases of Livestock Research Unit, USDA/Agricultural Research Service, National Animal Disease Center, Ames, Iowa 50010
K. B. REGISTER
Affiliation:
Respiratory Diseases of Livestock Research Unit, USDA/Agricultural Research Service, National Animal Disease Center, Ames, Iowa 50010
G. E. NORDHOLM
Affiliation:
Respiratory Diseases of Livestock Research Unit, USDA/Agricultural Research Service, National Animal Disease Center, Ames, Iowa 50010
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Abstract

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Fifty-seven bacterial isolates previously identified as Bordetella avium or B. hinzii were characterized by restriction enzyme analysis (REA) and/or ribotyping. Twenty restriction endonucleases were evaluated for REA. Digestion of chromosomal DNA from the 42 B. avium and 15 B. hinzii isolates with Hinf I produced 8 and 7 distinct fingerprint profiles, respectively. Digestion with DdeI further discriminated these Bordetella species and produced 12 fingerprint profiles for B. avium and 4 profiles of B. hinzii. In addition, B. avium isolates were clearly distinguishable from B. hinzii isolates by ribotyping with the restriction endonuclease PvuII. The ribotype patterns of these two species of Bordetella were unique when compared to previously reported ribotype patterns for B. bronchiseptica isolates. Since it was possible to discern differences among isolates within each Bordetella species by REA analysis, we suggest that REA could be used in developing a typing system based on the fingerprint profiles generated.

Type
Research Article
Information
Epidemiology & Infection , Volume 124 , Issue 1 , February 2000 , pp. 83 - 90
Copyright
© 2000 Cambridge University Press