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Restriction endonuclease characterization of resistant plasmids in Enterobacteriaceae isolated from children in the Sudan

Published online by Cambridge University Press:  15 May 2009

P. Shears ,
G. Suliman  and
C. A. Hart
P. Shears
Affiliation:
University Department of Medical Microbiology, Royal Liverpool Hospital, Liverpool L7 8XW, U.K.
G. Suliman
Affiliation:
Children's Emergency Hospital, P.O. Box 412, Khartoum, Sudan
C. A. Hart
Affiliation:
University Department of Medical Microbiology, Royal Liverpool Hospital, Liverpool L7 8XW, U.K.
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Summary

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The investigation of plasmid similarity is an important component in the surveillance of antimicrobial resistance and in the detection of epidemic plasmids. The use of restriction endonucleases in the classification of transferable, multiply-resistant plasmids from faecal Enterobacteriaceae isolated at the Children's Emergency Hospital, Khartoum was investigated. Twenty-four transconjugant plasmids, coding for 11 different resistance patterns, each of molecular weight 62 MDa. were studied using four restriction enzymes; Pst I, Eco R I, Hind III and Ara II. Fifteen different digest profiles were obtained. Restriction profiles discriminated between plasmids with differing resistance patterns and demonstrated homology of plasmids with common resistance patterns. Restriction endonuclease digest patterns provide a potentially rapid and reproducible method of plasmid classification, that could contribute towards surveillance systems in tropical countries with a high prevalence of antimicrobial resistance.

Type
Research Article
Information
Epidemiology & Infection , Volume 103 , Issue 3 , December 1989 , pp. 487 - 496
Copyright
Copyright © Cambridge University Press 1989

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