Hostname: page-component-586b7cd67f-t7fkt Total loading time: 0 Render date: 2024-11-26T05:36:25.775Z Has data issue: false hasContentIssue false
  • English
  • Français

Restricted variability of a 17 nucleotide stretch within the 5′-noncoding region of poliovirus genome

Published online by Cambridge University Press:  15 May 2009

T. Pöyry ,
L. Kinnunen  and
T. Hovi
T. Pöyry
Affiliation:
Enterovirus Laboratory, Department of Virology, and Molecular Biology Unit, Virology Group, National Public Health Institute, Helsinki, Finland
L. Kinnunen
Affiliation:
Enterovirus Laboratory, Department of Virology, and Molecular Biology Unit, Virology Group, National Public Health Institute, Helsinki, Finland
T. Hovi
Affiliation:
Enterovirus Laboratory, Department of Virology, and Molecular Biology Unit, Virology Group, National Public Health Institute, Helsinki, Finland
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The outbreak of poliomyelitis in Finland in 1984 was caused by a wild strain of poliovirus 3 with uncommon molecular and antigenic properties. We prepared a synthetic oligonucleotide probe complementary to nucleotides 494–510 in the 5′-noncoding part of the genome of a representative strain of the outbreak. This short nucleotide stretch was found to be relatively well conserved within the outbreak and uncommon among 82 independent poliovirus isolates. It may thus be a useful marker for screening isolates to identify those requiring more detailed genetic comparison. The sequences of the corresponding region of the genome are known for 32 separate poliovirus strains and 3 coxsackie B virus strains and show 6 fully conserved nucleotides that could assume a constant hairpin-loop position in a hypothetical secondary structure of the RNA. This could explain the persistence of a particular 17 nucleotide sequence for 40 years in nature in this highly variable region of the poliovirus genome.

Type
Research Article
Information
Epidemiology & Infection , Volume 103 , Issue 3 , December 1989 , pp. 671 - 683
Copyright
Copyright © Cambridge University Press 1989

References

REFERENCES

1.Hovi, T, Cantell, K, Huovilainen, A et al. Outbreak of paralytic poliomyelitis in Finland: widespread circulation of antigenically altered polioirus type 3 in a vaccinated population. Lancet 1986; i: 1427–32.CrossRefGoogle Scholar
2.Lapinleimu, K.Elimination of poliomyelitis in Finland. Rev Infect Dis 1984; 6 (Suppl. 2): 457–60.CrossRefGoogle ScholarPubMed
3.Huovilainen, A, Hovi, T., Kinnunen, L.Takkinen, K, Ferguson, M, Minor, P.Evolution of poliovirus during an outbreak: Sequential type 3 poliovirus isolates from several persons show shifts of neutralization determinants. J Gen Virol 1987; 68: 1373–8.CrossRefGoogle ScholarPubMed
4.Huovilainen, A.Kinnunen, L, Ferguson, M, Hovi, T.Antigenie variation among 173 strains of type-3-poliovirus/Finland/84–85. J Gen Virol 1988: 69: 1941–8.CrossRefGoogle Scholar
5.Magrath, DI.Evans, DMA.Ferguson, M. et al. Antigenic and molecular properties of type 3 poliovirus responsible for an outbreak of poliomyelitis in a vaccinated population. J Gen Virol 1986: 67: 899–905.CrossRefGoogle Scholar
6.Hughes, P.J.Evans, DMA.Minor, PD.Schild, GC.Almond, JW.Stanway, G.The nucleotide sequence of a type 3 poliovirus isolated during a recent outbreak of poliomyelitis in Finland. J Gen Virol 1986: 67: 2093–102.CrossRefGoogle ScholarPubMed
7.Kitamura, N.Semler, BL.Rothberg, PG. et al. Primary structure, gene organization and polypeptide expression of poliovirus RNA. Nature 1981: 291: 547–53.CrossRefGoogle ScholarPubMed
8.Racaniello, VR. & Baltimore, D.Molecular cloning of poliovirus cDNA and determination of the complete nucleotide sequence of the viral genome. Proc Natl Acad Sci USA 1981: 78: 4887–91.CrossRefGoogle ScholarPubMed
9.Stanway, GHughes, PJ.Mountford, RC et al. Comparison of the complete nucleotide sequences of the genomes of the neurovirulent poliovirus P3/Leon/37 and its attenuated Sabin vaccine derivative P3/Leon 12a1b. Proc Natl Acad Sci USA 1984: 81: 1539–43.CrossRefGoogle ScholarPubMed
10.Sanger, F.Nicklen, S.Coulson, AR.DNA sequencing with chainterminating inhibitors. Proc Natl Acad Sci USA 1977: 74: 5463–7.CrossRefGoogle ScholarPubMed
11.Biggin, MD.Gibson, TJ.Hong, GF.Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination. Proc Natl Acad Sci USA 1983; 80: 3963–5.CrossRefGoogle ScholarPubMed
12.Toyoda, H.Kohara, M.Kataoka, Y et al. Complete nucleotide sequences of all three poliovirus genomes. J Molec Biol 1984: 174: 561–85.CrossRefGoogle Scholar
13.Conner, B.J.Reyes, AA.Morin, C, Itakura, C, Teplitz, RL, Wallace, RB.Detection of sickle cell Bs-globin allele by hybridization with synthetic oliogonucleotides. Proc Natl Acad Sci USA 1983: 80: 278–82.CrossRefGoogle Scholar
14.Wallace, RB, Shaffer, J, Murphy, RF, Bonner, J, Hirose, T, Itakura, K.Hybridization of synthetic oligodeoxyribonucleotides to φx174 DNA: the effect of single base pair mismatch. Nucleic Acids Res 1979: 6: 3543–57.CrossRefGoogle ScholarPubMed
15.Cann, AJ.Stanway, G, Hughes, PJ et al. , Reversion to neurovirulence of the live attenuated Sabin type 3 oral poliovirus vaccine. Nucleic Acids Res 1984: 12: 7787–92.CrossRefGoogle ScholarPubMed
16.La Monica, N.Meriam, C, Racaniello, VR.Mapping of sequences required for mouse neurovirulence of poliovirus type 2 Lansing. J Virol 1986; 57: 515–25.CrossRefGoogle ScholarPubMed
17.Minor, PD.Dunn, G.The effect of sequences in the 5′ non-coding region on the replication of polioviruses in the human gut. J Gen Virol 1988; 69: 1091–6.CrossRefGoogle ScholarPubMed
18.Kuge, S, Nomoto, A.Construction of viable deletion and insertion mutants of the Sabin strain of type 1 poliovirus: Function of the 5′ noncoding sequence in viral replication. J Virol 1987; 61: 1478–87.CrossRefGoogle ScholarPubMed
19.Iizuka, N, Kuge, S, Nomoto, A.Complete nucleotide sequence of the genome of coxsackievirus B1. Virology 1987; 156: 64–73.CrossRefGoogle ScholarPubMed
20.Lindberg, AM, Stålhandske, POK.Pettersson, U.Genome of coxsackievirus B3. Virology 1987; 156: 50–63.CrossRefGoogle ScholarPubMed
21.Jenkins, O, Booth, JD, Minor, PD, Almond, JW.The complete nucleotide sequence of coxsachievirus B4 and its comparison to other members of the picornaviridae. J Gen Virol 1987; 68: 1835–48.CrossRefGoogle Scholar
22.Evans, DMA, Dunn, G, Minor, PD et al. Increased neurovirulence associated with a single nucleotide change in a noncoding region of the Sabin type 3 poliovaccine genome. Nature 1985; 314: 548–50.CrossRefGoogle Scholar
23.Salser, W.Glohin mRNA sequences: Analysis of base pairing and evolutionary implications. Cold Spring Harb Symp Quant Biol 1977; 42: 985–1002.CrossRefGoogle Scholar
24.Rivera, VM, Welsh, JD, Maizel, JV JrComparative sequence analysis of the 5′ noncoding region of the enteroviruses and rhinoviruses. Virology 1988; 165: 42–50.CrossRefGoogle ScholarPubMed