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The relative resistance of f2 bacteriophage to inactivation by disinfectants

Published online by Cambridge University Press:  15 May 2009

Murray Drulak ,
Alfred M. Wallbank  and
Ingrid Lebtag
Murray Drulak
Affiliation:
The Department of Medical Microbiology and Infectious Diseases, University of Manitoba Medical College, Winnipeg, Manitoba, Canada R3E 0W3
Alfred M. Wallbank
Affiliation:
The Department of Medical Microbiology and Infectious Diseases, University of Manitoba Medical College, Winnipeg, Manitoba, Canada R3E 0W3
Ingrid Lebtag
Affiliation:
The Department of Medical Microbiology and Infectious Diseases, University of Manitoba Medical College, Winnipeg, Manitoba, Canada R3E 0W3
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f2 bacteriophage in the presence of fetal calf serum (at a final concentration of 10%) was exposed to six commonly used disinfectants for times of 10, 20 and 30 sec. At the end of exposure times skim milk neutralized the disinfectant activity and residual virus was assayed using the plaque technique. The 6 disinfectants considered were Javex, sodium hydroxide, ethanol, Wescodyne, One Stroke Ves-Phene and Sonacide. A 0.25% (w/v) solution of sodium hydroxide and 1/50 Javex (1200 parts/106 chlorine) were the most effective of the six disinfectants considered since 105 f2 bacteriophage were inactivated in 30 seconds in each instance. Since a 0.25% (w/v) solution of sodium hydroxide had a pH of 12.5 this made it too caustic to use as a disinfectant in many practical situations. It was concluded therefore that Javex at some dilution less than 1/50 (greater than 1200 parts/106 chlorine) was the most practical of the six disinfectants to use. Ethanol (95%, v/v) inactivated 103 f2 bacteriophage in 30 seconds while 1/20 Wescodyne and undiluted Sonacide inactivated 101 virus particles. Ves-Phene at a dilution of 1/50 was a completely ineffective virucide during the 30 sec exposure. The resistance of f2 bacteriophage to inactivation by these six disinfectants was compared with that of echovirus 11 and coxsackievirus B5. In all instances except exposure to undiluted Sonacide, f2 was comparable in resistance to inactivation and in many cases had greater resistance.

Type
Research Article
Information
Journal of Hygiene , Volume 83 , Issue 1 , August 1979 , pp. 111 - 119
Copyright
Copyright © Cambridge University Press 1979

References

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