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Recovery of campylobacter from human faeces stored at 4 °C

Published online by Cambridge University Press:  15 May 2009

C. Ladrón de Guevara ,
M. T. Pérez-Pomata ,
A. Agulla ,
F. J. Merino ,
P. A. Villasante  and
A. C. Velasco
C. Ladrón de Guevara
Affiliation:
Servicio de Microbiología, Hospital ‘La Paz’, Castellana, 261, Madrid 28046, Spain
M. T. Pérez-Pomata
Affiliation:
Servicio de Microbiología, Hospital ‘La Paz’, Castellana, 261, Madrid 28046, Spain
A. Agulla
Affiliation:
Servicio de Microbiología, Hospital ‘La Paz’, Castellana, 261, Madrid 28046, Spain
F. J. Merino
Affiliation:
Servicio de Microbiología, Hospital ‘La Paz’, Castellana, 261, Madrid 28046, Spain
P. A. Villasante
Affiliation:
Servicio de Microbiología, Hospital ‘La Paz’, Castellana, 261, Madrid 28046, Spain
A. C. Velasco*
Affiliation:
Servicio de Microbiología, Hospital ‘La Paz’, Castellana, 261, Madrid 28046, Spain
*
*Dr A. C. Velasco.
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Six hundred and thirteen fresh diarrhoeal faeces were inoculated on Skirrow blood agar (SK), on Preston blood free agar (PBF), and in Campy-thioglycolate broth (CT). After 24 h of storage at 4 °C, specimens were again inoculated on SK and PBF, and in Campylobacter enrichment broth (CEB). CT tubes were placed overnight at 4 °C. Plates and CEB tubes were incubated at 43 °C in microaerophilic conditions. A total of 68 specimens was positive for campylobacter on direct plating. Sixty-four of them were also recovered after subculturing from CT, and only 51 from CEB. Delayed inoculation of plates after storage of samples at 4 °C yielded 57 isolates. The storage of faeces at 4 °C for 24 h significantly reduces the number of campylobacter isolates. When samples are not plated immediately we recommend inoculating a CT tube maintained at 4 °C overnight as a holding medium.

Type
Research Article
Information
Epidemiology & Infection , Volume 102 , Issue 2 , April 1989 , pp. 281 - 285
Copyright
Copyright © Cambridge University Press 1989

References

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