Hostname: page-component-6bf8c574d5-2jptb Total loading time: 0 Render date: 2025-02-16T16:46:03.823Z Has data issue: false hasContentIssue false
  • English
  • Français

Recovery of campylobacter from human faeces stored at 4 °C

Published online by Cambridge University Press:  15 May 2009

C. Ladrón de Guevara ,
M. T. Pérez-Pomata ,
A. Agulla ,
F. J. Merino ,
P. A. Villasante  and
A. C. Velasco
C. Ladrón de Guevara
Affiliation:
Servicio de Microbiología, Hospital ‘La Paz’, Castellana, 261, Madrid 28046, Spain
M. T. Pérez-Pomata
Affiliation:
Servicio de Microbiología, Hospital ‘La Paz’, Castellana, 261, Madrid 28046, Spain
A. Agulla
Affiliation:
Servicio de Microbiología, Hospital ‘La Paz’, Castellana, 261, Madrid 28046, Spain
F. J. Merino
Affiliation:
Servicio de Microbiología, Hospital ‘La Paz’, Castellana, 261, Madrid 28046, Spain
P. A. Villasante
Affiliation:
Servicio de Microbiología, Hospital ‘La Paz’, Castellana, 261, Madrid 28046, Spain
A. C. Velasco*
Affiliation:
Servicio de Microbiología, Hospital ‘La Paz’, Castellana, 261, Madrid 28046, Spain
*
*Dr A. C. Velasco.
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Six hundred and thirteen fresh diarrhoeal faeces were inoculated on Skirrow blood agar (SK), on Preston blood free agar (PBF), and in Campy-thioglycolate broth (CT). After 24 h of storage at 4 °C, specimens were again inoculated on SK and PBF, and in Campylobacter enrichment broth (CEB). CT tubes were placed overnight at 4 °C. Plates and CEB tubes were incubated at 43 °C in microaerophilic conditions. A total of 68 specimens was positive for campylobacter on direct plating. Sixty-four of them were also recovered after subculturing from CT, and only 51 from CEB. Delayed inoculation of plates after storage of samples at 4 °C yielded 57 isolates. The storage of faeces at 4 °C for 24 h significantly reduces the number of campylobacter isolates. When samples are not plated immediately we recommend inoculating a CT tube maintained at 4 °C overnight as a holding medium.

Type
Research Article
Information
Epidemiology & Infection , Volume 102 , Issue 2 , April 1989 , pp. 281 - 285
Copyright
Copyright © Cambridge University Press 1989

References

REFERENCES

Agulla, A., Merino, F. J., Villasante, P. A., Saz, J. V., Díaz, A. & Velasco, A. C. (1987). Evaluation of four enrichment media for isolation of Campylobacter jejuni. Journal of Clinical Microbiology 25, 174–175.CrossRefGoogle ScholarPubMed
Blankenship, L. C. & Craven, S. E. (1982). Campylobacter jejuni survival in chicken meat as a function of temperature. Applied and Environmental Microbiology 44, 88–92.CrossRefGoogle ScholarPubMed
Blaser, M. J., Berkowitz, I. D., LaForce, F. M., Cravens, J., Barth Reller, L. & Wang, W. L. L. (1979). Campylobacter enteritis: clinical and epidemiological features. Annals of Internal Medicine 91, 179–185.CrossRefGoogle Scholar
Blaser, M. J., Feldman, R. A. & Wells, J. G. (1982). Epidemiology of endemic and epidemic Campylobacter infections in the United States. In Campylobacter: Epidemiology, Pathogenesis and Biochemistry (ed Newell, D. G.), pp. 3–4. Lancaster: MTP Press.Google Scholar
Blaser, M. J., Hardesty, H. L., Powers, B. & Wang, W. L. L. (1980). Survival of Campylobacter fetus subsp. jejuni in biological milieus. Journal of Clinical Microbiology 13, 309–313.CrossRefGoogle Scholar
Chan, F. T. H. & Mackenzie, A. M. R. (1986). Evaluation of primary selective media and enrichment methods for Campylobacter species isolation. European Journal of Clinical Microbiology 5, 162–164.CrossRefGoogle ScholarPubMed
Doyle, H. P. & Roman, D. J. (1982). Recovery of Campylobacter jejuni and Campylobacter coli from inoculated foods by selective enrichment. Applied and Environmental Microbiology 43, 1343–1353.CrossRefGoogle ScholarPubMed
Grant, I. H., Richardson, N. J. & Bokkenheuser, V. D. (1980). Broiler chickens as potential source of Campylobacter infections in humans. Journal of Clinical Microbiology 11, 508–510.CrossRefGoogle ScholarPubMed
Goosens, H., De Boeck, M., Van Landuyt, H. & Butzler, J. P. (1984). Isolation of Campylobacter jejuni from human feces. In Campylobacter Infection in Man and Animals (ed. Butzler, J. P.), pp. 39–50. Boca Raton, Florida: CRC Press.Google Scholar
Humphrey, T. J. (1986). Techniques for the optimum recovery of cold injured Campylobacter jejuni from milk or water. Journal of Applied Bacteriology 61, 125–132.CrossRefGoogle ScholarPubMed
Humphrey, T. J. & Cruickshank, J. G. (1985). Antibiotic and deoxycholate resistance in Campylobacter jejuni following freezing or heating. Journal of Applied Bacteriology 59, 65–71.Google ScholarPubMed
Hutchinson, D. N. & Bolton, F. J. (1983). Is enrichment culture necessary for the isolation of Campylobacter jejuni from faeces? Journal of Clinical Pathology 36, 1350–1352.CrossRefGoogle ScholarPubMed
Hutchinson, D. N. & Bolton, F. J. (1984). Improved blood-free selective medium for the isolation of Campylobacter jejuni from faecal specimens. Journal of Clinical Pathology 37, 956–957.CrossRefGoogle ScholarPubMed
Luechtefeld, N. W., Wang, W. L. L., Blaser, M. J. & Barth Reller, L. (1981). Evaluation of transport and storage techniques for isolation of Campylobacter fetus subsp. jejuni from turkey fecal specimens. Journal of Clinical Microbiology 13, 438–443.CrossRefGoogle Scholar
Martin, W. T., Patton, C. M., Morris, G. K., Potter, M. E. & Puhr, N. D. (1983). Selective enrichment broth medium for isolation of Campylobacter jejuni. Journal of Clinical Microbiology 17, 853–855.CrossRefGoogle ScholarPubMed
Merino, F. J., Agulla, A., Villasante, P. A., Díaz, A., Saz, J. V. & Velasco, A. C. (1986). Comparative efficacy of seven selective media for isolating Campylobacter jejuni. Journal of Clinical Microbiology 24, 451–452.CrossRefGoogle ScholarPubMed
Morris, G. K. & Patton, C. M. (1985). Campylobacter. In Manual of Clinical Microbiology (ed. Lennette, E. H., Balows, A., Hausler, W. J. Jr, and Shadomy, H. J.), pp. 302–308. Washington, D.C.; American Society of Microbiology.Google Scholar
Ng, L-K., Stiles, M. E. & Taylor, D. E. (1985). Inhibition of Campylobacter coli and Campylobacter jejuni by antibiotics used in selective growth media. Journal of Clinical Microbiology 22, 510–514.CrossRefGoogle ScholarPubMed
Ray, B. & Johnson, C. (1984). Sensitivity of cold-stressed Campylobacter jejuni to solid and liquid selective environments. Food Microbiology 1, 173–176.CrossRefGoogle Scholar
Rothemberg, P. J., Stern, N. J. & Westhoff, D. C. (1984). Selected enrichment broth for recovery of Campylobacter jejuni from foods. Applied and Environmental Microbiology 48, 78–80.CrossRefGoogle Scholar
Rubin, S. J. & Woodard, M. (1983). Enhanced isolation of Campylobacter jejuni by cold enrichment in Campy-Thio broth. Journal of Clinical Microbiology 18, 1008–1010.CrossRefGoogle ScholarPubMed
Sjögren, E., Lindblom, G. B. & Kaijser, B. (1987). Comparison of different procedures, transport media, and enrichment media for isolation of Campylobacter species from healthy laying hens and humans with diarrhea. Journal of Clinical Microbiology 25, 1966–1968.CrossRefGoogle ScholarPubMed
Skirrow, M. B. (1977). Campylobacter enteritis: a ‘new’ disease. British Medical Journal 2, 9–11.CrossRefGoogle ScholarPubMed
Svedhem, Å, Kaijser, B. & Sjögren, E. (1981). The occurrence of Campylobacter jejuni in fresh food and survival under different conditions. Journal of Hygiene 87, 421–425.CrossRefGoogle ScholarPubMed
Tanner, E. I. & Bullin, C. H. (1977). Campylobacter enteritis. British Medical Journal 2, 579.CrossRefGoogle ScholarPubMed
Velasco, A. C., Mateos, M. L., Más, G., Pedraza, A., Díez, M. & Gutiérrez, A. (1984). Three-year prospective study of intestinal pathogens in Madrid, Spain. Journal of Clinical Microbiology 20, 290–292.CrossRefGoogle ScholarPubMed
Walder, M. (1982). Epidemiology of Campylobacter enteritis. Scandivanian Journal of Infectious Diseases 14, 27–33.CrossRefGoogle ScholarPubMed
Wang, W. L., Blaser, M. J. & Cravens, J. (1978). Isolation of Campylobacter. British Medical Journal 2, 57.CrossRefGoogle ScholarPubMed
Wang, W. L., Barth Reller, L., Smallwood, B., Luechtefeld, N. W. & Blaser, M. J. (1983). Evaluation of transport media for Campylobacter jejuni in human fecal specimens. Journal of Clinical Microbiology 18, 803–807.CrossRefGoogle ScholarPubMed