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The recovery of anaerobic bacteria from swabs

Published online by Cambridge University Press:  15 May 2009

J. G. Collee ,
B. Watt ,
R. Brown  and
Sandra Johnstone
J. G. Collee
Affiliation:
Department of Bacteriology, University of Edinburgh, Edinburgh, EH8 9AG
B. Watt
Affiliation:
Department of Bacteriology, University of Edinburgh, Edinburgh, EH8 9AG
R. Brown
Affiliation:
Department of Bacteriology, University of Edinburgh, Edinburgh, EH8 9AG
Sandra Johnstone
Affiliation:
Department of Bacteriology, University of Edinburgh, Edinburgh, EH8 9AG
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Summary

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When a standard sample of a simulated exudate containing known numbers of anaerobic bacteria was taken up on a swab and plated on solid medium, the number of colonies subsequently cultured represented a very small proportion of the original sample. Evidence is produced that the apparent loss is not primarily attributable to inactivation on the swab but rather to retention of organisms on the swab. This was demonstrable with Clostridium welchii and with Bacteroides species that have hitherto been regarded as relatively oxygen-sensitive.

When stock strains of Bacteroides species were held for some hours on swabs, some progressive loss of viability was demonstrable. A measure of protection was afforded when these organisms were held aerobically on blood agar medium, but a very exacting anaerobe and some wild strains of faecal anaerobes showed gradual inactivation under these conditions.

These findings may have important implications in relation to currently employed bacteriological sampling procedures with swabs in clinical practice.

Type
Research Article
Information
Journal of Hygiene , Volume 72 , Issue 3 , June 1974 , pp. 339 - 347
Copyright
Copyright © Cambridge University Press 1974

References

REFERENCES

Anderson, K. F. (1965). Antibacterial bacteriological swabs. British Medical Journal ii, 1003.CrossRefGoogle Scholar
Bartlett, D. I. & Hughes, M. H. (1969). Bacteriological swabs. British Medical Journal iii, 450.CrossRefGoogle Scholar
Beakley, J. W. (1957). The toxicity of wooden applicator sticks for Neisseria gonorrhoeae. Public Health Laboratory 15, 11.Google Scholar
Cary, S. G. & Blair, E. B. (1964). New transport medium for shipment of clinical specimens. I. Fecal specimens. Journal of Bacteriology 88, 96.CrossRefGoogle ScholarPubMed
Collee, J. G., Rutter, J. M. & Watt, B. (1971). The significantly viable particle: a study of the subculture of an exacting sporing anaerobe. Journal of Medical Microbiology 4, 271.CrossRefGoogle ScholarPubMed
Cooper, G. N. (1957). The prolonged survival of upper respiratory and intestinal pathogens on swabs. Journal of Clinical Pathology 10, 226.CrossRefGoogle ScholarPubMed
Crookes, E. M. & Stuart, R. D. (1959). The value of aerosporin in the isolation of Neisseriae from swabs forwarded to the laboratory in transport medium. Journal of Pathology and Bacteriology 78, 283.CrossRefGoogle Scholar
Cruickshank, R. (1953). Taking swabs. British Medical Journal ii, 1095.CrossRefGoogle Scholar
Dadd, A. H., Dagnall, V. P., Everall, P. H. & Jones, A. C. (1970). The survival of Streptococcus pyogenes on bacteriological swabs made from various fibres. Journal of Medical Microbiology 3, 561.CrossRefGoogle ScholarPubMed
Huet, M. & Bonnefous, S. (1960). Essai d'un milieu de transport et de conservation des selles pour l'isolement de Salmonella et Shigella par coprocultures. Archives Institut Pasteur Tunis 97, 37.Google Scholar
Mair, N. S. & McSwiggan, D. A. (1965). Antibacterial bacteriological swab. British Medical Journal ii, 230.Google Scholar
Pollock, M. R. (1947). The growth of H. pertussis on media without blood. British Journal of Experimental Pathology 28, 295.Google ScholarPubMed
Pollock, M. R. (1948). Unsaturated fatty acids in cotton wool plugs. Nature, London 161, 853.CrossRefGoogle ScholarPubMed
Rowatt, E. (1957). Some factors affecting the growth of Bordetella pertussis. Journal of General Microbiology 17, 279.CrossRefGoogle ScholarPubMed
Rubbo, S. D. & Benjamin, M. (1951). Some observations on survival of pathogenic bacteria on cotton-wool swabs: development of a new type of swab. British Medical Journal i, 983.CrossRefGoogle Scholar
Stuart, R. D. (1946). Diagnosis and control of gonorrhoea by bacteriological cultures, with preliminary report on new method for transporting clinical material. Glasgow Medical Journal 27, 131.Google ScholarPubMed
Stuart, R. D. (1956). Transport problems in public health bacteriology. The use of transport media and other devices to maintain the viability of bacteria in specimens. Canadian Journal of Public Health 47, 114.Google ScholarPubMed
Venkatraman, K. V. & Ramakrishnan, C. S. (1941). A preserving medium for the trans- mission of specimens for the isolation of Vibrio cholerae. Indian Journal of Medical Research 29, 681.Google Scholar
Watt, B. (1973). The influence of carbon dioxide on the growth of obligate and facultative anaerobes on solid media. Journal of Medical Microbiology 6, 307.Google Scholar
White, W. D. (1965). Antibacterial bacteriological swab. British Medical Journal ii, 229.CrossRefGoogle Scholar