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Rapid single-tube confirmatory test for Escherichia coli

Published online by Cambridge University Press:  25 March 2010

J. L. Smith  and
S. Rockliff
J. L. Smith
Affiliation:
Public Health Laboratory, Capital Territory Health Commission, Canberra, Australia
S. Rockliff
Affiliation:
Public Health Laboratory, Capital Territory Health Commission, Canberra, Australia
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Summary

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A single-tube confirmatory test that allows a result to be obtained in 4 h has been developed from the single-tube confirmatory test recommended by the Joint Committee of the Public Health Laboratory Service and the Standing Committee of Analysts (PHLS/SCA, 1980). A variety of river, lake and reservoir samples were examined for the presence of E. coli using either most probable number (MPN) or membrane filtration (MF) technique, and the PHLS/SCA recommended confirmatory medium (LTMB) was evaluated against traditional methods. To improve the performance of LTMB, the medium was modified and this modified medium when used in 0·1 ml volumes and incubated for 4 h at 44°C provided 99% agreement with traditional methods.

Type
Research Article
Information
Journal of Hygiene , Volume 89 , Issue 1 , August 1982 , pp. 149 - 154
Copyright
Copyright © Cambridge University Press 1982

References

REFERENCES

APHA (1975). Standard Methods for the Examination of Water and Wastewater, 13th ed.APHA AWWA WPCF.Google Scholar
Boyd, W. L. & Lichstein, H. C. (1955). The effect of carbohydrates on tryptophanase activity of bacteria. Journal of Bacteriology 69, 584–589.CrossRefGoogle ScholarPubMed
Oxoid (1979). The Oxoid Manual, 4th ed., pp. 214–215.Google Scholar
Public Health Laboratory Service/Standing Committee of Analysts (1980). Single tube confirmatory test for Escherichia coli. By a Joint Committee of the Public Health Laboratory Service and the Standing Committee of Analysts. Journal of Hygiene 85, 51.CrossRefGoogle Scholar
Report (1969). The bacteriological examination of water supplies. Report on Public Health and Medical Subjects, no. 71. London. H.M.S.O.Google Scholar