Hostname: page-component-cd9895bd7-7cvxr Total loading time: 0 Render date: 2024-12-22T16:30:33.340Z Has data issue: false hasContentIssue false
  • English
  • Français

Rapid inter-strain comparison by pyrolysis mass spectrometry of coagulase-negative staphylococci from persistent CAPD peritonitis

Published online by Cambridge University Press:  15 May 2009

R. Freeman ,
F. K. Gould ,
R. Wilkinson ,
A. C. Ward ,
N. F. Lightfoot  and
P. R. Sisson
R. Freeman*
Affiliation:
Department of Microbiology, University of Newcastle upon Tyne, Newcastle upon Tyne, UK
F. K. Gould
Affiliation:
Freeman Hospital, Newcastle upon Tyne, UK
R. Wilkinson
Affiliation:
Nephrology Unit, Freeman Hospital, Newcastle upon Tyne, UK
A. C. Ward
Affiliation:
Department of Microbiology, University of Newcastle upon Tyne, Newcastle upon Tyne, UK
N. F. Lightfoot
Affiliation:
Regional Public Health Laboratory, Newcastle upon Tyne, UK
P. R. Sisson
Affiliation:
Regional Public Health Laboratory, Newcastle upon Tyne, UK
*
Corresponding author: Dr R. Freeman, Department of Microbiology, Medical School, Newcastle upon Tyne, NE2 4HH.
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Pyrolysis mass spectrometry (PyMS) was used as a method of rapid inter-strain comparison of 19 isolates of coagulase-negative staphylococci from episodes of CAPD peritonitis. Thirteen isolates were from multiple, but distinct, episodes of peritonitis in 6 patients and the remaining 6 isolates were from 6 patients with single episodes. The results, expressed in terms of identity/non-identity of strains, were compared with those obtained using an established typing system comprising an extended antibiogram, determination of biotype and plasmid profile analysis. The PyMS results for inter-strain comparison were in agreement with the reference typing scheme results. PyMS can be used in this setting to rapidly obtain evidence that persistent infection is/is not likely to be due to the same organism, although it cannot be used for formal typing. The results by both methods showed that serial, apparently distinct, episodes of peritonitis over periods as long as 120 days may be due to the same strain of coagulase-negative staphylococcus. Clinically based distinctions between recurrence of infection (same strain) and re-infection (different strains) may not always be supported by the microbiological evidence.

Type
Special Article
Information
Epidemiology & Infection , Volume 106 , Issue 2 , April 1991 , pp. 239 - 246
Copyright
Copyright © Cambridge University Press 1991

References

REFERENCES

1.Keane, WF, Everett, ED, Fine, RN et al. , CAPD related peritonitis management and antibiotic therapy recommendations. Perit Dial Bull 1987; 7: 55–63.Google Scholar
2.Golper, TA, Geerlings, W, Selwood, NH et al. , Peritoneal dialysis results in the EDTA registry. In: Nolph, KD et al. , eds. Peritoneal dialysis. Dordrecht: Kluwer Acedemic Publishers, 1989: 414–28.Google Scholar
3.Working Party of the British Society for Antimicrobial Chemotherapy. Diagnosis and management of peritonitis in continuous ambulatory peritoneal dialysis. Lancet 1987; i: 845–9.Google Scholar
4.Ludlam, HA, Noble, WC, Marples, RR, Phillips, I. The evaluation of a typing scheme for coagulase-negative staphylococci suitable for epidemiological studies. J Med Microbiol 1989; 30: 161–5.CrossRefGoogle ScholarPubMed
5.Freeman, R, Goodfellow, M, Gould, FK, Hudson, SJ. Rapid epidemiological typing of clinical isolates of Staphylococcus epidermidis: a preliminary study with pyrolysis mass spectrometry. Zbl Bakt Hyg 1990; 21 (Suppl): 396–8.Google Scholar
6.Brown, D, Blowers, R. Disc methods of sensitivity testing and other semi-quantitative methods. In: Reeves, DS et al. , eds. Laboratory methods in antimicrobial chemotherapy. Edinburgh: Churchill Livingstone, 1978: 8–30.Google Scholar
7.Birnboim, HC, Doly, J. A rapid alkaline extraction procedure for screening recombinant DNA. Nucleic Acid Research 1979; 7: 1513–25.CrossRefGoogle Scholar
8.Meyers, JA, Sanchez, D, Elwell, P, Falkow, S. Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid. J Bact 1976; 127: 1529–37.Google Scholar
9.Aries, RE, Gutteridge, CS, Ottley, TW. Evaluation of a low-cost, automated pyrolysis mass spectrometer. J Analytical Appl Pyrolysis 1986; 9: 81–98.CrossRefGoogle Scholar
10.SPSSX User's Guide. New York: McGraw-Hill Book Co, 1983: 623–45.Google Scholar
11.Sokal, RR, Michener, CD. A statistical method for evaluating systematic relationships. University of Kansas Science Bulletin 1958; 38: 1409–38.Google Scholar
12.Vas, SI. Peritonitis. In: Nolph, KD et al. , eds. Peritoneal dialysis. Dordrecht: Kluwer Academic Publishers, 1989: 268–70.Google Scholar
13.Ludlam, HA, Noble, WC, Marples, RR, Bayston, R, Phillips, I. The epidemiology of peritonitis caused by coagulase-negative staphylococci in continuous ambulatory peritoneal dialysis. J Med Microbiol 1989; 30: 167–74.CrossRefGoogle ScholarPubMed
14.Freeman, R, Goodfellow, M, Gould, FK, Hudson, SJ, Lightfoot, NF. Pyrolysis-mass spectrometry (Py-MS) for the rapid epidemiological typing of clinically significant bacterial pathogens. J Med Microbiol 1990; 32: 283–6.Google Scholar
15.Magee, JT, Hindmarch, JM, Burnett, IA, Pease, A. Epidemiological typing of Streptococcus pyogenes by pyrolysis mass spectrometry. J Med Microbiol 1989; 30: 273–8.CrossRefGoogle ScholarPubMed
16.Voorhees, KJ, Durfee, SL, Updegraff, DM. Identification of diverse bacteria grown under diverse conditions using pyrolysis-mass spectrometry. J Microbiol Methods 1988; 8: 315–25.CrossRefGoogle Scholar