Hostname: page-component-586b7cd67f-r5fsc Total loading time: 0 Render date: 2024-11-25T07:09:30.310Z Has data issue: false hasContentIssue false
  • English
  • Français

RAPD analysis of isolates of Burkholderia pseudomallei from patients with recurrent melioidosis

Published online by Cambridge University Press:  15 May 2009

A. Haase ,
A. Melder ,
H. Smith-Vaughan ,
D. Kemp  and
B. Currie
A. Haase
Affiliation:
Menzies School of Health Research, P.O. Box 41096, Casuarina NT 0811, Australia
A. Melder
Affiliation:
Menzies School of Health Research, P.O. Box 41096, Casuarina NT 0811, Australia
H. Smith-Vaughan
Affiliation:
Menzies School of Health Research, P.O. Box 41096, Casuarina NT 0811, Australia
D. Kemp
Affiliation:
Menzies School of Health Research, P.O. Box 41096, Casuarina NT 0811, Australia
B. Currie
Affiliation:
Menzies School of Health Research, P.O. Box 41096, Casuarina NT 0811, Australia
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Twenty-seven isolates of Burholderia pseudomallei (formerly Pseudomonas pseudomallei) from ten patients with recurrent melioidosis were analysed by RAPD. In two cases RAPD patterns in recurrent isolates differed from the original isolates; one was considered a likely reinfection while the other may represent relapse from one of two strains initially infecting the patient. In two cases where a change in antibiotic resistance had occurred between original and relapse isolates, slight changes in RAPD patterns were found with one of the four primers used. In the other six cases the relapse was clearly due to the original strain re-emerging unchanged, with identical RAPD patterns with all four primers.

Type
Research Article
Information
Epidemiology & Infection , Volume 115 , Issue 1 , August 1995 , pp. 115 - 121
Copyright
Copyright © Cambridge University Press 1995

References

1.Rode, JW, Webling, DD. Melioidosis in the Northern Territory of Australia. Med J Aust 1981; 1: 181–4.CrossRefGoogle ScholarPubMed
2.Chaowagul, W, White, NJ, Dance, DAB et al. , Melioidosis: a major cause of community-acquired septicemia in northern Thailand. J Infect Dis 1989; 159: 890–9.CrossRefGoogle Scholar
3.Currie, B. Medicine in Tropical Australia. Med J Aust 1993; 158: 609–15.CrossRefGoogle ScholarPubMed
4.Desmarchelier, PM, Dance, DAB, Chaowagul, W, Supputamongkol, Y, White, NJ. Pitt, TL. Relationships among Pseudomonas pseudomallei isolates from patients with recurrent melioidosis. J Clin Microbiol 1993; 31: 1592–6.CrossRefGoogle ScholarPubMed
5.Morrison, RE, Lamb, AS, Craig, DB. Melioidosis: a reminder. Am J Med 1988; 84: 965–7.CrossRefGoogle ScholarPubMed
6.Bouvy, JJ, Degener, JE, Stijnen, C, Gallee, MP. Septic melioidosis after a visit to Southeast Asia. Eur J Clin Microbiol 1986; 5: 655–6.CrossRefGoogle ScholarPubMed
7.Leelarasamee, A, Bovornkitti, S. Melioidosis: review and update. Rev Infect Dis 1989; 11: 413–25.CrossRefGoogle Scholar
8.Weber, DR, Douglass, LE, Brundage, WG, Stallkamp, TC. Acute varieties of melioidosis occurring in US soldiers in Vietnam. Am J Med 1969; 46: 234.CrossRefGoogle ScholarPubMed
9.Wilks, D, Kim Jacobson, S, Lever, AM, Farrington, M. Fatal melioidosis in a tourist returning from Thailand. J Infect 1994; 29: 87–90.CrossRefGoogle Scholar
10.Mays, EE, Ricketts, EA. Melioidosis: recrudescence associated with bronchogenic carcinoma twenty-six years following initial geographic exposure. Chest 1976; 68: 261–3.CrossRefGoogle Scholar
11.Chaowagul, W, Supputtamongkol, Y, Dance, DAB, Rajchanuvong, A. Relapse in melioidosis: incidence and risk factors. J Infect Dis 1993; 168, 1181–5.Google ScholarPubMed
12.Currie, B, Howard, D, The, VN, Withnall, K, Merianos, A. The 1990–1991 outbreak of melioidosis in the Northern Territory of Australia: clinical aspects. S A J Trop Med Publ Health 1993; 24: 436–43.Google ScholarPubMed
13.Currie, B, Smith-Vaughan, H, Golledge, C, Buller, N, Sriprakash, KS, Kemp, DJ. Pseudomonas pseudomallei isolates collected over 25 years from a non-tropical endemic focus show clonality on the basis of ribotyping. Epidemiol Infect 1994; 113: 307–12.CrossRefGoogle ScholarPubMed
14.Williams, JGK, Kubelik, AR, Livak, KJ, Rafalski, JA, Tingley, SV. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucl Acids Res 1990; 18: 6531–5.CrossRefGoogle ScholarPubMed
15.Welsh, J, McClelland, M. Fingerprinting genomes using PCR with arbitrary primers. Nucl Acids Res 1990; 18: 7213–8.CrossRefGoogle ScholarPubMed
16.Akopyanz, N, Bukanov, BO, Westblom, TU. Kresovich, S. Berg, DE. DNA diversity among clinical isolates of Helicobacter pylori detected bv PCR-based RAPD fingerprinting. Nucl Acids Res 1992; 20: 5137–42.CrossRefGoogle Scholar
17.Kersulyte, D, Woods, JP, Keath, EJ, Goldman, WE, Berg, DE. Diversity among clinical isolates of Histoplasma capsulatum detected by polymerase chain reaction with arbitrary primers. J Bact 1992; 174: 7075–9.CrossRefGoogle ScholarPubMed
18.Haase, A, Smith-Vaughan, H, Melder, A et al. , Subdivision of Burkholderia pseudomallei ribotypes into multiple types by RAPD analysis provides new insights into its epidemiology. Submitted for publication.Google Scholar
19.Pitcher, DG, Saunders, NA, Owen, RJ. Rapid extraction of bacterial genomic DNA with guanidinium thiocyanate. Lett Appl Microbiol 1989; 8: 151–6.CrossRefGoogle Scholar
20.Lew, AE, Desmarchelier, PM. Molecular typing of Pseudomonas pseudomallei: restriction fragment length polymorphism of rRNA genes. J Clin Microbiol 1993; 31: 533–9.CrossRefGoogle ScholarPubMed
21.Weis, SE, Slocum, PC, Blais, FX et al. , The effect of directly observed therapy on the rates of drug resistance and relapse in tuberculosis. N Engl J Med 1994; 330: 1179–84.CrossRefGoogle ScholarPubMed