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The quantitative assay of bacterial aerosols by electrostatic precipitation

Published online by Cambridge University Press:  15 May 2009

E. H. Houwink
Affiliation:
Medical Biological Laboratory of the National Defence Research Council T.N.O., 139 Lange Kleiweg, Rijswijk-Z.H., The Netherlands
W. Rolvink
Affiliation:
Medical Biological Laboratory of the National Defence Research Council T.N.O., 139 Lange Kleiweg, Rijswijk-Z.H., The Netherlands
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1. The electrostatic precipitation of single cell bacterial aerosols has been studied. Two types of electrostatic precipitators of the wire-in-tube construction have been developed. In one type the collector is a tube with nutrient agar, in the other a vertical water-film.

2. Collection efficiencies of 90 % and more could be obtained at sampling rates of 25 l./min. and tensions varying from 5 to 10 kV.

3. The survival of organisms in electrostatic precipitators has been measured in comparison with other samplers, viz. the slit sampler, membrane filters and impingers. The equivalence of the electrostatic precipitators and other samplers in sampling spore aerosols could be demonstrated. Aerosols containing vegetative organisms were sampled equally well in the agar tube precipitator as in the slit sampler. In the precipitator with water-film collector there is a loss of c. 25 % of viable cells.

4. The practical advantage of the electrostatic precipitators is their very low air resistance.

The authors feel greatly indebted to Prof. Dr J. A. Cohen and Dr F. Wensinck for their helpful criticism of the manuscript, to Mr W. de Vries who designed and constructed the generator for extremely high tension; to Mr R. van Strik, Philips-Roxane (Weesp, Netherlands), for statistical advice and computations and to Miss H. Kranenburg for technical assistance.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1957

References

REFERENCES

Berry, C. M. (1941). Publ. Hlth Rep., Wash. 56, 2044.CrossRefGoogle Scholar
Bourdillon, R. B., Lidwell, O. M. & Thomas, J. C. (1941). J. Hyg., Camb., 41, 197.CrossRefGoogle Scholar
Chance, B., Hughes, V., McNicol, E. F., Sayre, D. & Williams, F. C. (1949). In Waveforms. New York: McGraw-Hill Book Co., Inc.Google Scholar
Deutsch, W. (1925). Z. tech. Phys. 6, 432.Google Scholar
Elford, W. J. & van den Ende, J. (1942). J. Hyg., Camb., 42, 240.CrossRefGoogle Scholar
Fuchs, N., Petrjanoff, I. & Rotzeig, B. (1936). Trans. Farad. Soc. 32, 1132.Google Scholar
Henderson, D. W. (1952). J. Hyg., Camb., 50, 53.CrossRefGoogle Scholar
Hollaender, A. & DallaValle, J. M. (1939). Publ. Hlth Rep., Wash., 54, 574.CrossRefGoogle Scholar
Jager, J. (1953). Data and circuits of television receiving valves. Amsterdam: Meulenhof and Co.Google Scholar
Luckiesh, M., Taylor, A. H. & Holladay, L. L. (1946). J. Bact. 52, 55.CrossRefGoogle Scholar
May, K. R. (1945). J. sci. Instrum. 22, 187.CrossRefGoogle Scholar
Orr, C., Gordon, M. T. & Kordecki, M. C. (1956). Appl. Microbiol. 4, 116.CrossRefGoogle Scholar
Pauthenier, M. M. & Moreau-Hanot, M. (1932). J. Phys. 3, 590.Google Scholar
Stearman, R. L. (1955). Bact. Rev. 19, 160.CrossRefGoogle Scholar
White, H. J. (1951). Trans. Amer. Inst. elect. Engrs, 70, 1186.CrossRefGoogle Scholar
Whittet, D. R. (1953). Report no. R 139 (Ministry of Supply), National Gas Turbine Establishment.Google Scholar
Wilcox, J. D. (1953). A. M. A. Arch. Ind. Hyg. Occup. Med. 7, 376.Google Scholar