Hostname: page-component-78c5997874-94fs2 Total loading time: 0 Render date: 2024-11-19T12:16:21.231Z Has data issue: false hasContentIssue false
  • English
  • Français

Pseudomonas pseudomallei isolates collected over 25 years from a non-tropical endemic focus show clonality on the basis of ribotyping

Published online by Cambridge University Press:  15 May 2009

B. Currie ,
H. Smith-Vaughan ,
C. Golledge ,
N. Buller ,
K. S. Sriprakash  and
D. J. Kemp
B. Currie*
Affiliation:
Menzies School of Health Research and Royal Darwin Hospital, Darwin, Australia
H. Smith-Vaughan
Affiliation:
Menzies School of Health Research and Royal Darwin Hospital, Darwin, Australia
C. Golledge
Affiliation:
Sir Charles Gairdner Hospital, Perth, Australia
N. Buller
Affiliation:
Western Australian Department of Agriculture, Perth, Australia
K. S. Sriprakash
Affiliation:
Menzies School of Health Research and Royal Darwin Hospital, Darwin, Australia
D. J. Kemp
Affiliation:
Menzies School of Health Research and Royal Darwin Hospital, Darwin, Australia
*
* Correspondence and reprints: Dr Bart Currie, Menzies School of Health Research, P.O. Box 41096. Casuarina NT 0811, Australia.
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Between 1966 and 1991, melioidosis, a disease caused by Pseudomonas pseudomallei that is mostly confined to tropical regions, occurred in farm animals and a farmer in temperate south-west Western Australia. Using an Escherichia coli probe containing a ribosomal RNA operon, P. pseudomallei DNA from isolates from 8 animals, a soil sample and the human case showed an identical ribotype on Southern blotting. The ribotype was different from the 3 commonest ribotypes seen in tropical Australia. This molecular typing supports the theory of clonal introduction of P. pseudomallei into a non-endemic region, with environmental contamination, local dissemination and persistence over 25 years. As melioidosis is often fatal in humans, such persistence in a temperate region is cause for concern.

Type
Research Article
Information
Epidemiology & Infection , Volume 113 , Issue 2 , October 1994 , pp. 307 - 312
Copyright
Copyright © Cambridge University Press 1994

References

REFERENCES

1.Leelarasamee, A, Bovornkitt, SMelioidosis: review and update. Rev Infect Dis 1989; 11: 413–25.CrossRefGoogle Scholar
2.Dance, DABMelioidosis: the tip of the iceberg? Clin Microbiol Rev 1991; 4: 52–60.CrossRefGoogle ScholarPubMed
3.Cottew, GSMelioidosis in sheep in Queensland. A description of the casual organism. Aust J Exp Biol Med Sci 1950; 28: 677–83.CrossRefGoogle Scholar
4.Rimington, RAMelioidosis in north Queensland. Med J Aust 1962; 1: 50–3.CrossRefGoogle Scholar
5.Currie, BJMedicine in tropical Australia. Med J Aust 1993; 158: 609–15.CrossRefGoogle ScholarPubMed
6.Ketterer, PJ, Bamford, VWA case of melioidosis in lambs in south Western Australia. Aust Vet J 1967; 43: 79–80.CrossRefGoogle Scholar
7.Golledge, CL, Chin, WS, Tribe, AE, Condon, RJ, Ashdown, LRA case of human melioidosis originating in south-west Western Australia. Med J Aust 1992; 157: 332–4.CrossRefGoogle ScholarPubMed
8.Lew, AE, Desmarchelier, PMMolecular typing of Pseudomonas pseudomallei: restriction fragment length polymorphisms of rRNA genes. J Clin Microbiol 1993; 31: 533–9.CrossRefGoogle ScholarPubMed
9.Pitcher, DG, Saunders, NA, Owen, RJRapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett Appl Microbiol 1989; 8: 151–6.CrossRefGoogle Scholar
10.Feinberg, AP, Vogelstein, BA technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 1983; 132: 6–13.CrossRefGoogle ScholarPubMed
11.Altwegg, M, Hickman-Brenner, FW, Farmer, JJRibosomal RNA gene restriction patterns provide increased sensitivity for typing Salmonella typhi strains. J Infect Dis 1989; 160: 145–9.CrossRefGoogle ScholarPubMed
12.Sutmoller, P, Kraneveld, FC, van der Schaaf, AMelioidosis (Pseudomalleus) in sheep, goats and pigs on Aruba Netherlands Antilles. J Am Vet Med Assoc 1957; 130: 415–7.Google Scholar
13.Mollaret, HH‘L'affaire du Jardin des plantes’ ou comment la melioidose fit son apparition en France. Med Mai Infect 1988; 18: 643–54.CrossRefGoogle Scholar
14.Ketterer, PJ, Donald, B, Rogers, RJBovine melioidosis in south-eastern Queensland. Aust Vet J 1975; 51: 395–8.CrossRefGoogle ScholarPubMed
15.Dance, DAB, King, C, Aucken, H, Knott, CD, West, PG, Pitt, TLAn outbreak of melioidosis in imported primates in Britain. Vet Rec 1992; 130: 525–9.CrossRefGoogle ScholarPubMed
16.Desmarchelier, PM, Dance, DAB, Chaowagul, W, Suputtamongkol, Y, White, NJ, Pitt, TLRelationships among Pseudomonas pseudomallei isolates from patients with recurrent melioidosis. J Clin Microbiol 1993; 31: 1592–6.CrossRefGoogle ScholarPubMed