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Protection of T4 bacteriophage against inactivation during freezing and thawing by addition of peptides

Published online by Cambridge University Press:  19 October 2009

P. R. M. Steele
P. R. M. Steele
Affiliation:
University Department of Pathology, Cambridge, England
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Summary

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Peptides of rabbit globin produced by tryptic digestion, were found to be highly protective against inactivation of freeze-thawed T4 phage. In concentrations of about 10−3 M the peptides protected the phage against inactivation by both concentrated NaBr in the unfrozen aqueous phase and the eutectic phase change.

Fractionation of the poptides by G25 Sephadex showed that peptide concentration rather than peptide size was the more important factor in determining the degree of protection of the phage by electrolyte effects. In contrast, protection against eutectic injury was strongly dependent on peptide size.

Possible mechanisms of action of the protective peptides are discussed.

Type
Research Article
Information
Journal of Hygiene , Volume 70 , Issue 3 , September 1972 , pp. 465 - 472
Copyright
Copyright © Cambridge University Press 1972

References

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