Hostname: page-component-cd9895bd7-gxg78 Total loading time: 0 Render date: 2024-12-22T16:55:01.543Z Has data issue: false hasContentIssue false
  • English
  • Français

Prospective study of verocytotoxin–producing, enteroaggregative and diffusely adherent Escherichia coli in different diarrhoeal states

Published online by Cambridge University Press:  15 May 2009

M. G. Brook ,
H. R. Smith ,
B. A. Bannister ,
M. McConnell ,
H. Chart ,
S. M. Scotland ,
A. Sawyer ,
M. Smith  and
B. Rowe
M. G. Brook*
Affiliation:
Departments of Infectious Diseases NHS Trust, London
H. R. Smith
Affiliation:
Laboratory of Enteric Pathogens, Central Public Health Laboratory, Public Health Laboratory Service, Colindale, London
B. A. Bannister
Affiliation:
Departments of Infectious Diseases NHS Trust, London
M. McConnell
Affiliation:
Laboratory of Enteric Pathogens, Central Public Health Laboratory, Public Health Laboratory Service, Colindale, London
H. Chart
Affiliation:
Laboratory of Enteric Pathogens, Central Public Health Laboratory, Public Health Laboratory Service, Colindale, London
S. M. Scotland
Affiliation:
Laboratory of Enteric Pathogens, Central Public Health Laboratory, Public Health Laboratory Service, Colindale, London
A. Sawyer
Affiliation:
Gastroenterology, Royal Free Hospital NHS Trust, London
M. Smith
Affiliation:
Gastroenterology, Royal Free Hospital NHS Trust, London
B. Rowe
Affiliation:
Laboratory of Enteric Pathogens, Central Public Health Laboratory, Public Health Laboratory Service, Colindale, London
*
*Correspondence and reprint requests: Department of Infectious and Tropical Diseases, Coppetts Wood Unit, Royal Free Hospital NHS Trust, Coppetts Road, London N10 1JN.
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

One hundred and eighty–one stool specimens from patients with various types of diarrhoea (135 patients) or from non-diarrhoeal controls (23 acute medical patients, 23 inflammatory bowel disease in remission) were investigated using a colony–blot DNA hybridization assay for the presence of Verocytotoxin–producing (VTEC), enteroaggregative (EAggEC) and diffusely adherent (DAEC) Escherichia coli. Twelve patients had probe–positive EAggEC in the stool and 8 of these had diarrhoea, 6 following recent travel. Eight patients had DAEC, 7 of whom had travellers diarrhoea. Six of 10 (60%) travellers with gastroenteritis, but without a recognized enteric pathogen, were positive for EAggEC (4) or DAEC (2). Five of 10 (50%) travellers with gastroenteritis related to a recognized enteric pathogen also had DAEC identified in their stool. Of the 23 acute medical control patients 11 had been abroad, 4 of these were immigrants and had EAggEC. VTEC were not found and, with one exception, immunoassays for antibodies to E. coli O 157 and O 2 lipopolysaccharides were negative.

Type
Research Article
Information
Epidemiology & Infection , Volume 112 , Issue 1 , February 1994 , pp. 63 - 67
Copyright
Copyright © Cambridge University Press 1994

References

REFERENCES

Levine, MM. Escherichia coli that cause diarrhoea: enterotoxigenic, enteropathogenic. enteroinvasive, enterohemorrhagic and enteroadherent. J Infect Dis 1987; 155: 377–89.CrossRefGoogle ScholarPubMed
Mathewson, JJ, Johnson, PP, Dupont, HL et al. , A newly recognised cause of travelers' diarrhea: enteroadherent Escherichia coli. J Infect Dis 1985; 151: 471–5.CrossRefGoogle ScholarPubMed
Cravioto, A, Tello, A, Navarro, A et al. , Association of Escherichia coli HEp-2 adherence patterns with type and patterns of diarrhea. Lancet 1991; 337: 262–4.Google Scholar
Giron, JA, Jones, T, Millan-Velasco, F et al. , Diffuse adhering Escherichia coli (DAEC) as a putative cause of diarrhoea in Mayan children in Mexico. J Infect Dis 1991; 163: 507–13.Google ScholarPubMed
Bhan, MK, Raj, P, Levine, MM et al. , Enteroaggregative Escherichia coli associated with persistent diarrhea in a cohort of rural children in India. J Infect Dis 1989; 159: 1061–3.CrossRefGoogle Scholar
Ostroff, SM, Kobayashi, JM, Lewis, JH. Infections with Escherichia coli O 157: H7 in Washington State. JAMA 1988; 262: 355–9.CrossRefGoogle Scholar
Karmali, MA. Infection by verocytotoxin producing Escherichia coli. Clin Microbiol Rev 1989; 2: 15–38.CrossRefGoogle ScholarPubMed
Chart, H, Smith, HR, Scotland, SM, Rowe, B, Milford, DW, Taylor, CM. Serological identification of Escherichia coli O 157: H7 infection in haemolytic uraemic syndrome. Lancet 1991: 337: 138–40.CrossRefGoogle Scholar
von Wullfen, H, Russmann, H, Karch, H et al. , Verocytotoxin-producing Escherichia coli O 2: H5 isolated from patients with ulcerative colitis. Lancet 1989; i: 1449–50.CrossRefGoogle Scholar
Willshaw, GA, Scotland, SM, Smith, HR, Rowe, B. Properties of Verocytotoxin-producing Escherichia coli of human origin belonging to serogroups other than O 157. J Infect Dis 1992; 166: 797–802.CrossRefGoogle Scholar
Baudry, B, Savarino, SJ, Vial, P, Kaper, JB, Levine, MM. A sensitive and specific DNA probe to identify enteroaggregative Escherichia coli, a recently discovered diarrhoeal pathogen. J Infect Dis 1990; 161: 1249–51.CrossRefGoogle Scholar
Scotland, SM, Willshaw, GA, Smith, HR, Gross, RJ, Rowe, B. Adhesion to cells in culture and plasmid profiles of enteropathogenic Escherichia coli isolated from outbreaks and sporadic cases of infant diarrhoea. J Infect 1989; 19: 237–49.Google Scholar
Chart, H, Scotland, SM, Rowe, B. Serum antibodies to Escherichia coli serotype O 157 in patients with haemolytic uraemic syndrome. J Clin Microbiol 1989; 27: 285–90.CrossRefGoogle Scholar
Walker, CW, Upson, R, Warren, RE. Haemorrhagic colitis: detection of verotoxin producing Escherichia coli in a clinical microbiological laboratory. J Clin Pathol 1988; 41: 80–4.CrossRefGoogle Scholar
Tarr, PI, Neill, MA, Clausen, CR et al. , Escherichia coli O 157 and the hemolytic uremic syndrome: importance of early culture in establishing the etiology. J Infect Dis 1990; 162: 553–6.Google Scholar
Karch, H, Meyer, T, Russmann, H, Heesemann, J. Frequent loss of shiga-like toxin genes in clinical isolates of Escherichia coli on subcultivation. Infect Immun 1992; 60: 3464–7.CrossRefGoogle ScholarPubMed
Nalin, DR. Enteric bacterial infections. Introduction and general principles. In: Strickland, GT. ed., Hunter's tropical medicine. London: WB Saunders, 1984: 270–9.Google Scholar
Tacket, CO, Moseley, SL, Kay, B et al. , Challenge studies in volunteers using Escherichia coli strains with diffuse adherence to HEp-2 cells. J Infect Dis 1990; 162: 550–2.CrossRefGoogle ScholarPubMed