Hostname: page-component-cd9895bd7-gbm5v Total loading time: 0 Render date: 2024-12-22T15:08:25.210Z Has data issue: false hasContentIssue false
  • English
  • Français

Proposed revisions to the serological typing system for Treponema hyodysenteriae

Published online by Cambridge University Press:  15 May 2009

D. J. Hampson ,
J. R. L. Mhoma ,
B. Combs  and
J. R. Buddle
D. J. Hampson
Affiliation:
School of Veterinary Studies, Murdoch University, Perth, Western Australia, 6150
J. R. L. Mhoma
Affiliation:
School of Veterinary Studies, Murdoch University, Perth, Western Australia, 6150
B. Combs
Affiliation:
School of Veterinary Studies, Murdoch University, Perth, Western Australia, 6150
J. R. Buddle
Affiliation:
School of Veterinary Studies, Murdoch University, Perth, Western Australia, 6150
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Antisera were prepared in rabbits against seven well-characterized strains of Treponema hyodysenteriae of known serotype, and reacted in agarose gel double immunodiffusion tests (AGDP) with lipopolysaccharide (LPS) extracted from 18 Western Australian isolates of the organism. Eight isolates were provisionally typed by this method, but sera raised against one ‘typed’ and two ‘untypable’ local isolates reacted in an unexpected fashion with LPS from other local and type strains. Serum raised against the ‘typed’ local isolate reached with LPS from other previously untyped local isolates: this indicated the presence of more than one major LPS antigen amongst certain local isolates, and was confirmed by cross-absorption of sera. Sera raised against apparently untypable local isolates reacted with LPS from certain type organisms, thus suggesting the presence of complex antigenic relationships between LPS antigens.

The serotyping system for T. hyodysenteriae which was proposed by Baum &Joens (1979) uses unabsorbed antisera and is made unworkable by these observations. Instead we propose placing organisms which share common LPS antigens into serogroups A to E, members of which are defined by their reactivity with unabsorbed sera raised against a type organism for the group. We suggest strains B78, WAI, B169, Al and WA6 respectively as being the most suitable type organisms for the five serogroups identified so far. Isolates possessing additional unique LPS antigens can be regarded as serotypes within the serogroup. However the serotype of an isolate can only be established if antiserum is prepared against it, and this serum continues to react homologously after cross-absorption with bacteria from other serotypes within the serogroup.

Type
Research Article
Information
Epidemiology & Infection , Volume 102 , Issue 1 , February 1989 , pp. 75 - 84
Copyright
Copyright © Cambridge University Press 1989

References

REFERENCES

Baum, D. H. & Joens, L. A. (1979). Serotypes of beta-haemolytic Treponema hyodysenteriae. Infection and Immunity 25, 792–796.CrossRefGoogle ScholarPubMed
Dubois, M., Giles, K. A., Hamilton, J. K., Rebers, P. D. & Smith, T. (1956). Colorimetric method for determination of sugars and related substances. Analytical Chemistry 28, 350–356.CrossRefGoogle Scholar
Egan, I. T., Harris, D. L. & Joens, L. A. (1983). Comparison of the microtitration agglutination test and the enzyme-linked immunosorbent assay for the detection of herds infected with swine dysentery. American Journal of Veterinary Research 44, 1323–1328.Google ScholarPubMed
Harris, D. L., Glock, R. D., Christensen, C. R. & Kinyon, J. M. (1972). Swine dysentery. I. Inoculation of pigs with Treponema hyodysenteriae (new species) and reproduction of the disease. Veterinary Medicine and Small Animal Clinician 67, 61–64.Google Scholar
Hovind-Hougen, K. & Hogh, P. (1984). Morphological and serological heterogeneity among strongly hemolytic spirochaetes isolated from pigs with swine dysentery. Proceedings of the International Pig Veterinary Society Congress, Ghent. p. 181.Google Scholar
Joens, L. A., Nord, N. A., Kinyon, J. M. & Egan, I. T. (1982). Enzyme linked immunosorbent assay for detection of antibody to Treponema hyodysenteriae antigens. Journal of Clinical Microbiology 15, 249–252.CrossRefGoogle ScholarPubMed
Kinyon, J. M. & Harris, D. L. (1979). Treponema innocens, a new species of intestinal bacteria. and emended description of the type strain of Treponem hyodysenteriae. International Journal of Systematic Bacteriology 29, 102–109.CrossRefGoogle Scholar
Kinyon, J. M., Harris, D. L. & Glock, R. D. (1977). Enteropathogenicity of various isolates of Treponema hyodysenteriae. Infection and Immunity 15, 638–646.CrossRefGoogle ScholarPubMed
Kunkle, R. A., Harris, D. L. & Kinyon, J. M. (1986). Autoclaved liquid medium for propogation of Treponema hyodysenteriae. Journal of Clinical Microbiology 24, 669–671.CrossRefGoogle Scholar
Lemcke, R. M. & Bew, J. (1984). Antigenic differences among isolates of Treponema hyodysenteriae. Proceedings of the International Pig Veterinary Society Congress. Ghent. p. 183.Google Scholar
Mapother, M. E. & Joens, L. A. (1985). New serotypes of Treponema hyodysenteriae. Journal of Clinical Microbiology 22, 161–164.CrossRefGoogle ScholarPubMed
Taylor, D. J. & Alexander, T. J. L. (1971). The production of dysentery in swine by feeding cultures containing a spirochaete. British Veterinary Journal 127, 1 8–1 9.CrossRefGoogle ScholarPubMed
Westphal, O., Lüderitz, D. & Bister, R. (1952). Uber die extraktion von bakterien mit phenol/wasser. Z. Naturforsch 76, 148–155.CrossRefGoogle Scholar