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Production of monoclonal antibodies to hepatitis B surface and core antigens, and use in the detection of viral antigens in liver biopsies

Published online by Cambridge University Press:  19 October 2009

R. S. Tedder ,
P. Guarascio ,
J. L. Yao ,
R. B. Lord  and
A. L. W. F. Eddleston
R. S. Tedder
Affiliation:
The Department of Virology, Middlesex Hospital Medical School, London W1.
P. Guarascio
Affiliation:
Liver Unit, King's College Hospital, London SE9
J. L. Yao
Affiliation:
The Department of Virology, Middlesex Hospital Medical School, London W1.
R. B. Lord
Affiliation:
The Department of Virology, Middlesex Hospital Medical School, London W1.
A. L. W. F. Eddleston
Affiliation:
Liver Unit, King's College Hospital, London SE9
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Summary

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Hybridomas secreting monoclonal antibodies to HBsAg and HBcAg were prepared from immunized mice. An antibody capture radioimmunoassay was used to detect and select appropriate hybrids for propagation and cloning. The advantages of this assay were discussed. The resulting monoclonal antibodies were compared with conventional polyclonal antisera for the detection of virus antigens in liver tissue and found to give excellent results.

Type
Research Article
Information
Journal of Hygiene , Volume 90 , Issue 1 , February 1983 , pp. 135 - 142
Copyright
Copyright © Cambridge University Press 1983

References

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