Hostname: page-component-78c5997874-j824f Total loading time: 0 Render date: 2024-11-05T04:24:25.891Z Has data issue: false hasContentIssue false
  • English
  • Français

The production and inactivation of pyocines

Published online by Cambridge University Press:  15 May 2009

A. H. Wahba
A. H. Wahba
Affiliation:
Cross-Infection Reference Laboratory, Central Public Health Laboratory, Colindale, London, N. W. 9
Rights & Permissions [Opens in a new window]

Extract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Certain strains of Ps. aeruginosa produce, in addition to pyocines, substances which inhibit pyocine activity. These pyocine inhibitors are probably proteolytic enzymes.

In order to investigate the production of pyocines by various strains of Ps. aeruginosa, aeruginosa, nutrient agar medium was devised in which the action of the pyocine-inhibiting substances is suppressed by incorporating 10–5M iodoacetic acid, 0·1 % sodium citrate and 0·1 % dipotassium hydrogen phosphate. This medium also diminished slime production.

Pyocine production is a stable characteristic which is not lost on repeated sub-culture or prolonged storage, and might form the basis of a typing system for Ps. aeruginosa.

I wish to thank Dr M. T. Parker, Director of the Cross-Infection Reference Laboratory, Public Health Laboratory Service, for constant encouragement and helpful advice.

Type
Research Article
Information
Journal of Hygiene , Volume 61 , Issue 4 , December 1963 , pp. 431 - 441
Copyright
Copyright © Cambridge University Press 1963

References

Abbott, J. D. & Shannon, R. (1958). A method for typing Shigella sonnei using colicine production as a marker. J. din. Path. 11, 71.CrossRefGoogle ScholarPubMed
Brubaker, R. R. & Sutrgalla, M. J. (1961). Pesticins. 1. Pesticin–bacterium inter-relation-ships and environmental factors influencing activity. J. Bact. 82, 940.CrossRefGoogle Scholar
Elek, S. D. (1953). Studies on the Proteoclast (‘Muller's phenomenon’). J. Hyg., Camb. 51, 125.CrossRefGoogle ScholarPubMed
Elliott, S. D. (1945). A proteolytic enzyme produced by group A streptococci with special reference to its effect on the type specific M antigen. J. exp. Med. 81, 573.CrossRefGoogle Scholar
Emmerich, R. & Löw, O. (1899). Bakteriolytische Enzyme als lJrsache der erworbenen Immunität und die Heilung von Infektionskrankheiten durch dieselben. Z. Hyg. InfektKr. 31, 1.Google Scholar
Fisher, E. (1960). Some properties of a proteinase obtained from Ps. aerugznosa. Bact. Proc. 60.Google Scholar
Frédéricq, P. (1948). Sur la destruction des colicines par les protéases microbiennes. C.R. Soc. Biol., Paris, 142, 403.Google Scholar
Gorini, L. (1950). Le role du calcium dans l'activité et la stabilité de quelques protéases bactériennes. Biochim. Biophys. Acta, 6, 237.CrossRefGoogle Scholar
Gratia, A. & Betz-Bareau, Mme. (1946). La pluralité des colicines et leur sensibilité variable á la trypsine et aux protéases microbiennes. C.R. Soc. Biol., Paris, 140, 1248.Google Scholar
Haihes, R. B. (1932). The influence of medium on the production of bacterial gelatinase. Biochem. J. 26, 323.Google Scholar
Hamon, Y. (1956). Contribution a l'étude des pyocines. Ann. Inst. Pasteur, 91, 82.Google ScholarPubMed
Hamon, Y., Véron, M. & Péron, Y. (1961). Contribution a l'´tude des propri´t´s lysogènes et bactériocinogènes dans le genre Pseudomonas. Ann. Inst. Pasteur, 101, 738.Google Scholar
Hamon, Y. & Péron, Y. (1962). Étude du pouvoir bactériocinogène dans le genre Listeria. Ann. Inst. Pasteur, 103, 876.Google Scholar
Hays, E. E., Wells, I. C., Katzman, P. A., Cain, C. K., Jacons, F. A., Thayer, S. A., Doisy, E. A., Gaby, W. L., Roberts, E. C., Muir, R. D., Carroll, C. J., Jones, L. R. & Wade, N. J. (1945). Antibiotic substances produced by Pseudomonas aeruginosa. J. biol. Chern. 159, 725.CrossRefGoogle Scholar
Holland, I. B. (1962). Further observations on the properties of megacin, a bacteriocine formed by B. megaterium. J. gen. Microbiol. 29, 603.CrossRefGoogle Scholar
Holloway, B. W. (1960). Grouping Pseudomonas aeruginosa by lysogenicity and pyocinogenicity. J. Path. Bact. 80, 448.CrossRefGoogle ScholarPubMed
Jacob, F. (1954). Biosynthèse induite et mode d'action d'une pyocine, antibiotique de Pseudornonas pyocyanea. Ann. Inst. Pasteur, 86, 149.Google Scholar
Jacob, F., Lwoff, A., Siminovitch, A. & Wollmann, E. (1953). Définitions de quelques termes relatifs à la lysog´nie. Ann. Inst. Pasteur, 84, 222.Google Scholar
Lominski, I., Smith, D. D. & Morrison, R. B. (1953). A coagulase-destroying factor produced by variants of Staphylococcus aureus. Nature, Lond., 171, 214.CrossRefGoogle ScholarPubMed
Maschmann, E. (1937). Über Bakterienproteasen. Biochern. Z. 294, 1.Google Scholar
Papavassiliou, J. (1961). Actions antibiotiques r´ciproques chez Pseudomonas aeruginosa. Arch. Inst. Pasteur, Tunis, 38, 57.Google Scholar
Schoental, R. (1941). The nature of the antibacterial agents present in Pseudomonas pyocyanea cultures. Brit. J. exp. Path. 22, 137.Google Scholar
Young, G. (1947). Pigment production and antibiotic activity in cultures of Pseudomonas aeruginosa. J. Bact. 54, 109.CrossRefGoogle ScholarPubMed