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Prevalence of peritonitis-associated coagulase-negative staphylococci on the skin of continuous ambulatory peritoneal dialysis patients

Published online by Cambridge University Press:  15 May 2009

M. A. Beard-Pegler ,
C. L. Gabelish ,
E. Stubbs ,
C. Harbour ,
J. Robson ,
M. Falk ,
R. Benn  and
A. Vickery
M. A. Beard-Pegler
Affiliation:
Department of Infectious Diseases, University of Sydney, NSW 2006, Australia
C. L. Gabelish
Affiliation:
Department of Infectious Diseases, University of Sydney, NSW 2006, Australia
E. Stubbs
Affiliation:
Department of Infectious Diseases, University of Sydney, NSW 2006, Australia
C. Harbour
Affiliation:
Department of Infectious Diseases, University of Sydney, NSW 2006, Australia
J. Robson
Affiliation:
Department of Microbiology, Royal Prince Alfred Hospital, Sydney, NSW 2050, Australia
M. Falk
Affiliation:
Department of Microbiology, Royal Prince Alfred Hospital, Sydney, NSW 2050, Australia
R. Benn
Affiliation:
Department of Microbiology, Royal Prince Alfred Hospital, Sydney, NSW 2050, Australia
A. Vickery
Affiliation:
Department of Microbiology, Royal Prince Alfred Hospital, Sydney, NSW 2050, Australia
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The predominance of coagulase-negative staphylococci as normal skin flora is thought to be a factor in their association with episodes of peritonitis in patients undergoing continuous ambulatory peritoneal dialysis. We investigated the prevalence of peritonitis-associated strains on the skin of 28 patients undergoing peritoneal dialysis. Coagulase-negative staphylococci were the most frequently isolated organisms. comprising 47% of peritoncal dialysis fluid isolates and 59% of body site isolates. A total of 142 coagulase-negative staphylococci were speciated. tested for their antimicrobial sensitivity and slime production. and identified by phage typing and plasmid-profile analysis. Staphylococcus epidermidis was the most commonly identified species from both peritoncal dialysis fluid (73%) and body sites (53%). Multiple antibiotic resistance was common, and the greater proportion of isolates were resistant to methicillin: 63·6% of peritoncal dialysis fluid isolates and 61·7% of body-site isolates.isolates. S. haemolyticus isolates were significantly more resistant to methicillin than other species. By phage typing and plasmid-profile analysis it was shown that peritonitis was rarely caused by skincolonizing strains. In only 3 of 14 patients were peritonitis-associated strains isolated as skin colonizers, and no patients developed peritonitis due to organisms previously isolated as skin colonizers.

Type
Special Article
Information
Epidemiology & Infection , Volume 102 , Issue 3 , June 1989 , pp. 365 - 378
Copyright
Copyright © Cambridge University Press 1989

References

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