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The Precipitation Reaction: Optimal Proportions, Neutrality and Maximal Precipitation in Mixtures of Albumin and Antiserum

Published online by Cambridge University Press:  15 May 2009

G. L. Taylor
Affiliation:
From the Department of Pathology, the Low Temperature Station and the Biochemical Laboratory, Cambridge.
G. S. Adair
Affiliation:
From the Department of Pathology, theLow Temperature Station and the Biochemical Laboratory, Cambridge.
Muriel E. Adair
Affiliation:
From the Department of Pathology, theLow Temperature Station and the Biochemical Laboratory, Cambridge.
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Culbertson (1932) has recently described a method for estimating the antibody content of antisera against crystalline egg-albumin. He determined by tests on supernatant fluids the point at which antigen and antibody neutralised each other. At this neutralisation point he found that antigen and antibody combined in a ratio of 1 to 13, and that the amount of precipitate nitrogen was maximal. The ratio 1 to 13, he stated, held for all the precipitates in the post-zone where antibody was in excess.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1934

References

REFERENCES

Adair, G. S. and Robinson, Muriel E. (1930). The specific refraction increments of serum-albumin and serum-globulin. Biochem. J. 24, 993.CrossRefGoogle ScholarPubMed
Culbertson, J. T. (1932). A quantitative study of the precipitin reaction with special reference to crystalline egg albumin and its antibody. J. Immunol. 23, 439.CrossRefGoogle Scholar
Dean, H. R. and Webb, R. A. (1926). The influence of optimal proportions of antigen and antibody in the serum precipitation reaction. J. Path. and Bact. 29, 473.CrossRefGoogle Scholar
Duncan, J. T. (1932). The use of equivalent proportions of antigen and serum in absorption of precipitin. Brit. J. Exp. Path. 13, 489.Google Scholar
Duncan, J. T. (1932). The relation of optimal agglutination to the equivalent serum-suspension ratio. Brit. J. Exp. Path. 13, 498.Google Scholar
Marrack, J. and Smith, F. C. (1931). Quantitative aspects in immunity reactions: the composition of the precipitate in precipitin reactions. Brit. J. Exp. Path. 12, 182.Google Scholar
Miles, A. A. (1933). Optimal proportions in agglutination: with reference to the Brucella group of organisms. Brit. J. Exp. Path. 14, 43.Google Scholar
Parnas, J. K. and Wagner, R. (1921). Über die Ausführung von Bestimmungen kleiner Stickstoffmengen nach Kjeldahl. Biochem. Zeitschr. 125, 253.Google Scholar
Sørensen, S. P. L. (1930). The constitution of soluble proteins. Compt. Rend. lab. Carlsberg, 18, 45.Google Scholar
Journ. of Hyg. XXXIV 9Google Scholar
Svedberg, T. (1930). Ultrazentrifugale Dispersitätsbestimmungen an Eiweisslösungen. Kolloid-Zeitschr. 51, 10.CrossRefGoogle Scholar
Taylor, G. L. (1931). The results of some quantitative experiments on the serum precipitation reaction. J. Hygiene, 31, 56.CrossRefGoogle ScholarPubMed
Taylor, G. L. (1933). The dissimilarity of the results of precipitin titrations performed with a constant amount of antiserum and with a constant amount of antigen. J. Hygiene 33, 12.CrossRefGoogle ScholarPubMed
Taylor, G. L., Adair, G. S. and Adair, Muriel E. (1932). The estimation of proteins by the precipitation reaction. J. Hygiene 32, 340.CrossRefGoogle ScholarPubMed
Topley, W. W. C. (1933). Outline of Immunity. London: Arnold.Google Scholar