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The post-mortem diagnosis of influenzal infection by fluorescent IgG, IgA and IgM antibody studies on necropsy blood

Published online by Cambridge University Press:  15 May 2009

L. M. de Silva ,
M. S. Khan ,
G. Kampfner ,
J. O'H. Tobin ,
R. Gillett  and
C. A. Morris
L. M. de Silva
Affiliation:
Public Health Laboratory, Withington Hospital, Manchester M20 8LR
M. S. Khan
Affiliation:
Public Health Laboratory, Withington Hospital, Manchester M20 8LR
G. Kampfner
Affiliation:
Public Health Laboratory, Withington Hospital, Manchester M20 8LR
J. O'H. Tobin
Affiliation:
Public Health Laboratory, Withington Hospital, Manchester M20 8LR
R. Gillett
Affiliation:
Hope Hospital, Salford
C. A. Morris
Affiliation:
Public Health Laboratory, Royal Salop Infirmary Group Laboratories, Mytton Oak Road, Shrewsbury, S Y3 8XH
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Summary

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Necropsy blood from cases diagnosed as dying from influenza A was examined for specific antibody in the IgG, IgA and IgM fractions and a specific diagnosis of recent infection was made if either IgM or IgA antibody and low titres of IgG antibody were found. By these criteria a diagnostic rate of 77% was found in those cases from whom no virus was isolated. The use of infected cell monolayers grown on polytetrafluoroethylene-coated slides gave a simple method of carrying out these antibody assays, and the use of necropsy blood did not require any special methods of transport of specimens to the virus laboratory.

Type
Research Article
Information
Journal of Hygiene , Volume 71 , Issue 1 , March 1973 , pp. 107 - 112
Copyright
Copyright © Cambridge University Press 1973

References

REFERENCES

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