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Population genetics of Mycobacterium tuberculosis complex in Scotland analysed by pulsed-field gel electrophoresis

Published online by Cambridge University Press:  15 May 2009

E. S. Olson*
Affiliation:
Department of Medical Microbiology, University of Aberdeen, Aberdeen AB9 2ZD
K. J. Forbes
Affiliation:
Department of Medical Microbiology, University of Aberdeen, Aberdeen AB9 2ZD
B. Watt
Affiliation:
Scottish Mycobacteria Reference Laboratory, City Hospital, Edinburgh EH10 5SB
T. H. Pennington
Affiliation:
Department of Medical Microbiology, University of Aberdeen, Aberdeen AB9 2ZD
*
*Dr E. S. Olson, Department of Medical Microbiology, St Bartholomew's Hospital, West Smithfield, London EC1A 7BE.
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The results of typing of 121 strains in the Mycobacterium tuberculosis complex by PFGE are presented. Every isolate from patients in Scotland over a 3-month period for M. tuberculosis and for 1 year for M. bovis were included along with several laboratory strains including those of BCG. The PFGE results suggest that the population structure of all the strains in this complex is distinctly simple with limited genetic diversity and also suggest that M. bovis is not a distinct species.

Type
Special Article
Copyright
Copyright © Cambridge University Press 1995

References

REFERENCES

1.Pennington, CI. Mortality, public health and medical improvement in Glasgow 1855–1911. PhD thesis. Stirling, Scotland: University of Stirling, 1977.Google Scholar
2.Watt, B. Scottish Mycobacteria Reference Laboratory Report for 1992.Google Scholar
3.Lévy-Frébault, VV, Portaels, F. Proposed minimal standards for the genus Mycobacterium and for the description of new slowly growing Mycobacterium species. Int J Syst Bacteriol 1992; 42: 315–23.CrossRefGoogle ScholarPubMed
4.Yates, MD, Collins, CH, Grange, JM. ‘Classical’ and ‘Asian’ variants of Mycobacterium tuberculosis isolated in Southeast England 1977–80. Tubercle 1982; 62: 5561.Google Scholar
5.Collins, CH, Yates, MD, Grange, JM. Subdivision of Mycobacterium tuberculosis into five variants for epidemiological purposes: methods and nomenclature. J Hyg 1982; 89: 235–42.CrossRefGoogle ScholarPubMed
6.Barrow, PA. Aspects of the epidemiology of bovine tuberculosis in badgers and cattle. II. The development and use of a typing system for Mycobacterium bovis. J Hyg 1981; 86: 247–57.Google Scholar
7.Barrow, PA. Physiological characteristics of the Mycobacterium tuberculosis–M. bovis group of organisms with particular reference to heterogeneity within M. bovis. J Gen Microbiol 1986; 132: 427–30.Google ScholarPubMed
8.Roman, MC, Sicilia, MJL. Preliminary investigation of Mycobacterium tuberculosis biovars J Clin Microbiol 1984; 20: 1015–6.CrossRefGoogle ScholarPubMed
9.Hoffner, SE, Svenson, SB, Norberg, R, Dias, F, Ghebremichael, S, Källenius, G. Biochemical heterogeneity of Mycobacterium tuberculosis complex isolates in Guinea-Bissau. J Clin Microbiol 1993; 31: 2215–7.CrossRefGoogle ScholarPubMed
10.Grange, JM, Aber, VR, Allen, BW, Mitchison, DA, Goren, MB. The correlation of bacteriophage types of Mycobacterium tuberculosis with guinea-pig virulence and in vitro-indicators of virulence. J Gen Microbiol 1978; 108: 17.CrossRefGoogle ScholarPubMed
11.Wietan, G, Haverkamp, J, Meuzelaar, HLC, Engel, HWB, Berwald, LC. Pyrolysis mass spectrometry: A new method to differentiate between the mycobacteria of the ‘Tuberculosis complex’ and other mycobacteria. J Gen Microbiol 1981; 122: 109–18.Google Scholar
12.Floyd, MH, Silcox, VA, Jones, WD Jr, Butler, WR, Kilburn, JO. Separation of Mycobacterium bovis BCG from Mycobacterium tuberculosis and Mycobacterium bovis by using high-performance lipid chromatography of mycolic acids. J Clin Microbiol 1992; 30: 1327–30.Google Scholar
13.Millership, SE, Want, SV. Whole-cell protein electrophoresis for typing Mycobacterium tuberculosis. J Clin Microbiol 1992; 30: 2784–7.Google Scholar
14.Cousins, DV, Francis, BR, Gow, BL et al. , Tuberculosis in captive seals: Bacteriological studies on an isolate belonging to the Mycobacterium tuberculosis complex. Res Vet Sci 1990; 48: 196200.CrossRefGoogle Scholar
15.Fevre, A, Fougerat, J, Brunneau, S, Guinet, R.Characterisation of Mycobacterium species by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Appl Theor Electrophor 1991; 2: 13–6.Google Scholar
16.Thompson, PJ, Cousins, DV, Gow, BL, Collins, DM, Williamson, BH, Dagnia, HT. Seals, seal trainers and mycobacterial infection. Am Rev Respir Dis 1993; 147: 164–7.Google Scholar
17.Cousins, DV, Williams, SN, Renter, R et al. , Tuberculosis in wild seals and characterisation of the seal bacillus. Aust Vet J 1993; 70: 92–7.CrossRefGoogle ScholarPubMed
18.Collins, DM, De Lisle, GW. DNA Restriction endonuclease analysis of Mycobacterium tuberculosis and Mycobacterium bovis BCG. J Gen Microbiol 1984; 130: 1019–21.Google Scholar
19.Collins, DM, De Lisle, GW. DNA restriction endonuclease analysis of Mycobacterium bovis and other members of the tuberculosis complex. J Clin Microbiol 1985; 21: 562–4.CrossRefGoogle ScholarPubMed
20.Collins, DM, De Lisle, GW, Gabric, DM. Geographic distribution of restriction types of Mycobacterium bovis isolates from brush-tailed possums (Trichosurus vulpecula) in New Zealand. J Hyg 1986; 96: 431–8.Google Scholar
21.Collins, DM, De Lisle, GW. BCG identification by DNA restriction fragment patterns. J Gen Microbiol 1987; 133: 1431–4.Google Scholar
22.Labidi, A, Thoen, CO. Genetic relatedness among Mycobacterium tuberculosis and M. bovis. Acta Leprol 1989; 7 (Suppl 1): 217–21.Google Scholar
23.Soolingen, D Van, Haas, PEW De, Hermans, PWM, Groenen, PMA, Van Embden, JDA. Comparison of various repetitive DNA elements as genetic markers for strain differentiation and epidemiology of Mycobacterium tuberculosis. J Clin Microbiol 1993; 31: 1987–95.CrossRefGoogle ScholarPubMed
24.Van Embden, JDA, Cave, MD, Crawford, JT et al. , Strain identification of Mycobacterium tuberculosis by DNA fingerprinting: recommendations for a standardised methodology. J Clin Microbiol 1993; 31: 406–9.Google Scholar
25.Cheverel-Dellagi, D, Abderrahman, A, Haltiti, R, Koubaji, H, Gicquel, B, Dellagi, K. Largescale DNA fingerprinting of Mycobacterium tuberculosis strains as a tool for epidemiological studies of tuberculosis. J Clin Microbiol 1993; 31: 2446–50.Google Scholar
26.Romling, U, Grothues, D, Heuer, T, Tummler, B. Physical genome analysis of bacteria. Electrophoresis. 1992; 13: 626–31.CrossRefGoogle ScholarPubMed
27.Lévy-Frébault, VV, Thorel, M, Varnerot, A, Gicquel, B. DNA polymorphism in Mycobacterium paratuberculosis, ‘Wood pigeon Mycobacteria’ and related mycobacteria analysed by field inversion gel electrophoresis. J Clin Microbiol 1989; 27: 2823–6.CrossRefGoogle Scholar
28.Fomokung, NG, Dale, JW, Osborn, TW, Grange, JM. Use of genetic probes based on insertion sequence IS986 to differentiate between BCG vaccine strains. J Appl Bacteriol 1992; 72: 126–33.CrossRefGoogle Scholar
29.Zhang, Y, Mazurek, GH, Cave, MD et al. , DNA polymorphisms in strains of Mycobacterium tuberculosis analysed by pulsed-field gel electrophoresis a tool for epidemiology. J Clin Microbiol 1992; 30: 1551–6.CrossRefGoogle Scholar
30.Hector, JSR, Pang, Y, Mazurek, GH, Zhang, Y, Brown, BA, Wallace, RJ Jr. Large restriction fragment patterns of genomic Mycobacterium fortuitum DNA as strain-specific markers and their use in the epidemiologic investigation of four nosocomial outbreaks. J Clin Microbiol 1992; 30: 1250–5.CrossRefGoogle ScholarPubMed
31.Varnerot, A, Clément, F, Gheorghiu, M, Lévy-Frébault, VV. Pulsed field gel electrophoresis of representatives of Mycobacterium tuberculosis and Mycobacterium bovis BCG strains. FEMS Microbiol Letts 1992; 98: 155–60.Google Scholar
32.Kristjansson, M, Green, P, Manning, HL et al. , Molecular confirmation of Bacillus Calmette-Guérin as the cause of pulmonary infection following urinary tract installation. Clin Infect Dis 1993; 17: 228–30.Google Scholar
33.Coffin, JW, Condon, C, Compston, CA, Potter, KN, Lamontagne, , Shafiq, J, Kunimoto, DY. Use of restriction fragment polymorphisms resolved by pulsed-field gel electrophoresis for subspecies identification of mycobacteria in the Mycobacterium avium complex and for isolation of DNA probes. J Clin Microbiol 1992; 30: 1829–36.Google Scholar
34.Mazurek, GH, Hartman, S, Zhang, Y et al. , Large DNA restriction fragment polymorphism in the Mycobacterium avium-M. intracellulare complex: a potential epidemiologic tool. J Clin Microbiol 1993; 31: 390–4.CrossRefGoogle ScholarPubMed
35.Arbeit, RD, Slutsky, A, Barber, TW et al. , Genetic diversity among strains of Mycobacterium avium causing monoclonal and polyclonal bacteremia in patients with AIDS. J Infect Dis 1993; 167: 1384–90.CrossRefGoogle ScholarPubMed
36.Wallace, RJ Jr, Zhang, Y, Brown, BA, Fraser, V, Mazurek, GH, Maloney, S. DNA large restriction fragments of sporadic and epidemic nosocomial strains of Mycobacterium chelonae and Mycobacterium abscessus. J Clin Microbiol 1993; 31: 2697–701.Google Scholar
37.Dice, LR. Measurement of the amount of ecologic association between species. Ecology 1945; 26: 297302.Google Scholar
38.Von Reyn, CF, Maslow, JN, Barber, TW, Falkinham, JO IIIrd, Arbeit, RD. Persistent colonisation of potable water as a source of Mycobacterium avium infection in AIDS. Lancet 1994; 343: 1137–41.CrossRefGoogle ScholarPubMed
39.Thierry, D, Cave, MD, Eisenach, KD, Crawford, JT, Bates, JH, Gicquel, B, Guesdon, JL. IS6110 and IS-like element of Mycobacterium tuberculosis complex. Nucleic Acids Res 1990; 18: 188.Google Scholar
40.Collins, DM, Earsmun, SK, Stephens, DM, Yates, GF, De Lisle, GW. DNA fingerprinting of Mycobacterium bovis strains by restriction fragment analysis and hybridisation with insertion elements IS1081 and IS6110. J Clin Microbiol 1993; 31: 1143–7.Google Scholar
41.Baess, I. Deoxyribonucleic acid relatedness among species of slowly-growing mycobacteria. Acta Path Microbiol Scand Sect B 1979; 87: 221–6.Google Scholar
42.Imaeda, T. Deoxyribonucleic acid relatedness among selected strains of Mycobacterium tuberculosis, Mycobacterium bovis, Mycobacterium bovis BCG, Mycobacterium microti and Mycobacterium africanum. Int J Syst Bact 1985; 35: 147–50.CrossRefGoogle Scholar
43.Frothingham, R, Hills, HG, Wilson, KH. Extensive DNA sequence conservation throughout the Mycobacterium tuberculosis complex. J Clin Microbiol 1994; 32: 1639–43.Google Scholar
44.Tsukamura, M, Mizuno, M, Toyama, H. Taxonomic studies on the Mycobacterium tuberculosis series. Microbiol Immunol 1985; 29: 285–99.Google Scholar
45.Fomukong, NG, Dale, JW. Transpositional activity of IS986 in Mycobacterium smegmatis. Gene 1993; 130: 99105.Google Scholar