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Polyethylene glycol purification of influenza virus with respect to aggregation and antigenicity

Published online by Cambridge University Press:  15 May 2009

A. Polson ,
A. Keen ,
C. Sinclair-Smith  and
I. G. S. Furminger
A. Polson
Affiliation:
M.R.C. and U.G.T. Virus Research Unit, Medical School, Observatory, Cape Town
A. Keen
Affiliation:
M.R.C. and U.G.T. Virus Research Unit, Medical School, Observatory, Cape Town
C. Sinclair-Smith
Affiliation:
M.R.C. and U.G.T. Virus Research Unit, Medical School, Observatory, Cape Town
I. G. S. Furminger
Affiliation:
Evans Medical Limited, Speke, Liverpool
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Influenza virus may be precipitated and aggregated by polyethylene glycol into clusters comprising ten to many hundred virions per aggregate. These aggregates are sparingly soluble and may be freed of contaminating polymer by washing in the appropriate buffer at room temperature or by precipitation in 30 % (v/v) ethanol at subzero temperatures.

Immunogenic studies in guinea-pigs of the virus in different states of dispersion revealed that the aggregated virus is the superior antigen to the virus in the monomeric form or in the dissociated state following treatment with ether.

Type
Research Article
Information
Journal of Hygiene , Volume 70 , Issue 2 , June 1972 , pp. 255 - 265
Copyright
Copyright © Cambridge University Press 1972

References

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