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Plasmid profile analysis and antimicrobial susceptibility patterns of shigella isolates from Nigeria

Published online by Cambridge University Press:  15 May 2009

D. K. Olukoya  and
O. Oni
D. K. Olukoya
Affiliation:
National Institute for Medical Research, PMB 2013, Yaba, Lagos, Nigeria
O. Oni
Affiliation:
National Institute for Medical Research, PMB 2013, Yaba, Lagos, Nigeria
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Summary

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In an epidemiological survey, plasmid profiles and antimicrobial susceptibility testing of 100 shigella isolates in Lagos, Nigeria was done. All the isolates were sensitive to nalidixic acid, nitrofurantoin and ciprofloxacin. The commonest antimicrobial susceptibility pattern was resistance to ampicillin, colistin sulphate, co-trimoxazole, streptomycin and tetracycline. All but 4 of 100 isolates screened contained one or more plasmids. Plasmid profile analysis distinguished more strains than did antimicrobial susceptibility patterns. A total of 36 isolates was able to transfer resistance plasmids to Escherichia coli K-12 by conjugation. Using in vitro transformation, seven isolates transferred resistance. These plasmids specified resistance to tetracycline, streptomycin, sulphonamide, trimethoprim and ampicillin.

Type
Special Article
Information
Epidemiology & Infection , Volume 105 , Issue 1 , August 1990 , pp. 59 - 64
Copyright
Copyright © Cambridge University Press 1990

References

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