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Plasmid analysis of Salmonella enteritidis isolated from human gastroenteritis cases and from epidemiologically associated poultry flocks

Published online by Cambridge University Press:  15 May 2009

C. R. Dorn ,
R. Silapanuntakul ,
E. J. Angrick  and
L. D. Shipman
C. R. Dorn
Affiliation:
Department of Veterinary Preventive Medicine, The Ohio State University, 1900 Coffey Road, Columbus, OH 43210
R. Silapanuntakul
Affiliation:
Department of Veterinary Preventive Medicine, The Ohio State University, 1900 Coffey Road, Columbus, OH 43210
E. J. Angrick
Affiliation:
Department of Veterinary Preventive Medicine, The Ohio State University, 1900 Coffey Road, Columbus, OH 43210
L. D. Shipman
Affiliation:
Veterinary Services, U.S. Department of Agriculture, 2301 N. Cameron Street, Harrisburg, PA 17110
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Summary

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Plasmid analysis of Salmonella enteritidis isolates from human gastroenteritis cases and from two commercial egg-producing poultry flocks was performed to determine if the poultry flocks were the source of the human infections. The plasmid profile and restriction fragment pattern (fingerprint) of five S. enteritidis isolates from human cases matched those of nine isolates from internal organs of egg-laying hens in one flock which was the source of eggs consumed by the cases. Another commercial flock was epidemiologically associated as the source of eggs consumed by affected persons in four separate gastroenteritis outbreaks from which S. enteritidis isolates were available. Five S. enteritidis isolates from human cases in these four outbreaks had the same profile and fingerprint, and they all matched those of the 24 isolates from hens in this flock. These results provide further documentation of egg-borne transmission of S. enteritidis to humans.

Type
Research Article
Information
Epidemiology & Infection , Volume 111 , Issue 2 , October 1993 , pp. 239 - 244
Copyright
Copyright © Cambridge University Press 1993

References

REFERENCES

1.St Louis, MC, Morse, DL, Potter, ME, et al. The emergence of grade A eggs as a major source of Salmonella enteritidis infections. JAMA 1988; 259: 2103–7.CrossRefGoogle Scholar
2. Anonymous. Salmonella enteritidis phage type 4: Chicken and egg. Lancet 1988; ii: 720–2.Google Scholar
3.Rodrigue, DC, Tauxe, RV, Rowe, B. International increase in Salmonella enteritidis: a new pandemic? Epidemiol Infect 1990; 105: 21–7.CrossRefGoogle ScholarPubMed
4.Centers for Disease Control. Outbreak of Salmonella enteritidis associated with consumption of raw shell eggs, 1991. MMWR 1992; 41: 369–72.Google Scholar
5.Gast, RK. Beard, CW. Production of Salmonella enteritidis - contaminated eggs by experimentally infected hens. Avian Dis 1990; 34: 438–46.CrossRefGoogle ScholarPubMed
6.Hinton, M. Pearson, GR, Threlfall, EJ, Rowe, B, Woodward, M, Wray, C. Experimental Salmonella enteritidis infection in chicks. Vet Rec 1989; 124: 223.CrossRefGoogle ScholarPubMed
7.Shivaprasad, HL, Timoney, JF, Morales, S, Lucio, B, Baker, RC. Pathogenesis of Salmonella enteritidis infection in laying chickens. I. Studies of egg transmission, clinical signs, fecal shedding, and serological responses. Avian Dis 1990; 34: 548–57.CrossRefGoogle Scholar
8.Timoney, JF. Shivaprasad, HL, Baker, RC, Rowe, B. Egg transmission after infection of hens with Salmonella enteritidis phage type 4. Vet Rec 1989; 125: 600–1.Google ScholarPubMed
9.Wachsmuth, K. Molecular epidemiology of bacterial infections: examples of methodology and of investigations of outbreaks. Rev Infect Dis 1986; 8: 682–92.CrossRefGoogle ScholarPubMed
10.Threlfall, EJ, Rowe, B, Ward, LR. Subdivision of Salmonella enteritidis phage types by plasmid profile typing. Epidemiol Infect 1989; 102: 459–65.CrossRefGoogle ScholarPubMed
11.Dorn, CR. Silapanuntakul, R, Angrick, EJ, Shipman, LD. Plasmid analysis and epidemiology of Salmonella enteritidis infection in three commercial layer flocks. Avian Dis 1992; 36: 844–51.CrossRefGoogle ScholarPubMed
12.Rivera, MJ, Rivera, N, Castillo, J, Rubio, MC, Gomez-Lus, R. Molecular and epidemiological study of Salmonella clinical isolates. J Clin Microbiol 1991; 29: 927–32.CrossRefGoogle ScholarPubMed
13.Rodrigue, DC, Cameron, DN, Puhr, ND, et al. Comparison of plasmid profiles, phage types, and antimicrobial resistance patterns of Salmonella enteritidis isolates in the United States. J Clin Microbiol 1992; 30: 854–7.CrossRefGoogle ScholarPubMed
14.U.S. Department of Agriculture. Poultry affected by Salmonella enteritidis; interim rule. Federal Register 1990; 55: 5576–84.Google Scholar
15.Hickman-Brenner, FW, Stubbs, AD, Farmer, JJ, III.Phage typing of Salmonella enteritidis in the United States. J Clin Microbiol 1991; 29: 2817–23.CrossRefGoogle Scholar
16.Marcina, FL, Kopecko, DJ, Jones, KR, Ayers, DJ, McCowen, SM. A multiple plasmid-containing Escherichia coli strain convenient source of size reference plasmids molecules. Plasmid 1978; 1: 417–20.Google Scholar