Hostname: page-component-586b7cd67f-tf8b9 Total loading time: 0 Render date: 2024-11-22T12:02:13.737Z Has data issue: false hasContentIssue false
  • English
  • Français

PCR-based detection of chlamydial infection in swine and subsequent PCR-coupled genotyping of chlamydial omp1-gene amplicons by DNA-hybridization, RFLP-analysis, and nucleotide sequence analysis

Published online by Cambridge University Press:  02 January 2001

L. E. HOELZLE ,
G. STEINHAUSEN  and
M. M. WITTENBRINK
L. E. HOELZLE
Affiliation:
Institute for Medical Microbiology, Infectious and Epidemic Diseases, Ludwig-Maximilians-University Munich, Germany Institute of Veterinary Bacteriology, University of Zurich, Zurich, Switzerland
G. STEINHAUSEN
Affiliation:
Clinic of Internal Medicine and Surgery of Swine, Ludwig-Maximilians-University Munich, Germany
M. M. WITTENBRINK
Affiliation:
Institute of Veterinary Bacteriology, University of Zurich, Zurich, Switzerland
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Lung and intestine of 49 pigs with respiratory diseases and endocervical swabs from 205 sows with reproductive disorders were investigated for chlamydial infection by polymerase chain reaction. PCR primers targeted DNA sequences on the chlamydial omp1 or omp2 genes. PCR amplicons were generated from 49·0% of pigs with respiratory disease, from 60·0% of sows with reproductive disorders, from 24·5% of respiratory healthy controls, but from no endocervical swabs from fertile sows. By DNA hybridization, a high prevalence of mixed infections with Chlamydophila abortus and Chlamydia suis in the porcine lung and intestine was found and confirmed by RFLP and nucleotide analysis. Of the omp1-PCR amplicons from endocervical swabs 81·3% were identified as Chlamydophila abortus, indicating an association of this chlamydial species with reproductive disorders in sows. Nucleotide sequence analysis of omp1-amplicons identified as deriving from Chlamydia suis shared a maximum of 82·7% homology with the reference strain S45.

Type
Research Article
Information
Epidemiology & Infection , Volume 125 , Issue 2 , October 2000 , pp. 427 - 439
Copyright
© 2000 Cambridge University Press