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The pathogenicity of thymidine kinase-deficient mutants of herpes simplex virus in mice

Published online by Cambridge University Press:  15 May 2009

H. J. Field  and
P. Wildy
H. J. Field
Affiliation:
Division of Virology, Department of Pathology, Tennis Court Road, Cambridge CB2 1QP
P. Wildy
Affiliation:
Division of Virology, Department of Pathology, Tennis Court Road, Cambridge CB2 1QP
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The pathogenicity for mice of two mutants of herpes simplex virus (type 1 and type 2), which fail to induce thymidine kinase, were compared with their respective parent strains. The mutants were much less virulent than the parents following either intracerebral or peripheral inoculation. The replication of the virus at the site of inoculation and its progression into the nervous system were studied. Following a very large inoculum in the ear, the type 1 mutant was found to establish a latent infection in the cervical dorsal root ganglia. Mice inoculated intracerebrally with small doses of the mutant viruses were solidly immune to challenge with lethal doses of the parent strain.

Type
Research Article
Information
Journal of Hygiene , Volume 81 , Issue 2 , October 1978 , pp. 267 - 277
Copyright
Copyright © Cambridge University Press 1978

References

REFERENCES

Coons, A. H. & Kaplan, M. H. (1950). Localization of antigen in tissue cells. II.Improvements in a method for the detection of antigen by means of fluorescent antibody. Journal of Experimental Medicine 91, 1–13.Google Scholar
Dubbs, D. R. & Kit, S. (1964). Mutant strains of herpes simplex deficient in thymidine kinase-inducing ability. Virology 22, 493–502.CrossRefGoogle Scholar
Field, H. J. & Hill, T. J. (1974). The pathogenesis of pseudorabies in mice following peripheral inoculation. Journal of General Virology 23, 145–57.CrossRefGoogle ScholarPubMed
Finney, D. J. (1952). In Statistical Methods in Biological Assay, pp. 524–53. London: Griffin.Google Scholar
Godfrey, R. C. (1972). Resistance to intracerebral challenge in mice immunized against herpes simplex virus. British Journal of Experimental Pathology 53, 529–39.Google ScholarPubMed
Hill, T. J., Field, H. J. & Blyth, W. A. (1975). Acute and recurrent infection with herpes simplex virus in the mouse: a model for studying latency and recurrent disease. Journal of General Virology 28, 341–53.CrossRefGoogle Scholar
Jamieson, A. T., Gentry, G. A. & Subak-Sharpe, J. H. (1974). Induction of both thymidine and deoxycytidine kinase activity by herpes viruses. Journal of General Virology 24, 465–80.Google Scholar
Klemperer, H. G., Haynes, G. R., Shedden, W. I. H. & Watson, D. H. (1967). A virus specific thymidine kinase in BHK21 cells infected with herpes simplex virus. Virology 31, 120–28.Google Scholar
Lofgren, K. W., Stevens, J. G., Marsden, H. S. & Subak-Sharpe, J. H. (1977). Temperature-sensitive mutants of herpes simplex virus differ in the capacityto establish latent infections in mice. Virology 76, 440–43.CrossRefGoogle Scholar
Marcialis, M. A., La Colla, P., Schivo, M. L., Flore, O., Firinu, A. & Loddo, B. (1975). Low virulence and immunogenicity in mice and in rabbits of variants of herpes simplex virus resistant to 5-iodo-2-deoxyuridine. Experientia 31, 502–3.CrossRefGoogle ScholarPubMed
Oakes, J. E. (1975). Role for cell-mediated immunity in the resistance of mice to subcutaneous herpes simplex virus infection. Infection and Immunity 12, 166–72.CrossRefGoogle ScholarPubMed
Price, R. W., Katz, B. J. & Notkins, A. L. (1975). Latent infection of the peripheral autonomic nervous system with herpes simplex virus. Nature, London 257, 686–8.CrossRefGoogle ScholarPubMed
Russell, W. C. (1962). A sensitive and precise plaque assay for herpes virus. Nature, London 195,1928–9.CrossRefGoogle ScholarPubMed
Thouless, M. E. (1972). Serological properties ofthymidine kinase produced in cells infected with type 1 or type 2 herpes virus. Journal of General Virology 17, 307–15.CrossRefGoogle ScholarPubMed
Thouless, M. E. & Wildy, P. (1975). Deoxypyrimidine kinases of herpes simplex viruses types 1 and 2: comparison of serological and structural properties. Journal of General Virology 26, 159–70.CrossRefGoogle ScholarPubMed
Vantsis, T. J. & Wildy, P. (1962). Interaction of herpesvirus and HeLa cells: comparison of cell killing and infective center formation. Virology 17, 225–32.CrossRefGoogle ScholarPubMed
Watson, D. H., Shedden, W. I. H.Elliot, A., Tetsuka, T., Wildy, P., Bourgaux-Ramoisy, D. & Gold, E. (1966). Virus specific antigens in mammalian cells infected with herpes simplex virus. Immunology 11, 399–408.Google ScholarPubMed
Zygraich, N. & Huygelen, C. (1973). In vivo behaviour of a temperature sensitive (ts) mutant of herpesvirus hominis type 2. Archiv für die gesamte Virusforschung 43, 103–11.CrossRefGoogle ScholarPubMed