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The optimal mode of transport for swabs obtained from surfaces examined for organisms causing food-borne disease

Published online by Cambridge University Press:  15 May 2009

D. A. A. Mossel ,
E. H. Kampelmacher  and
L. M. V. Noorle Jansen
D. A. A. Mossel
Affiliation:
Laboratory for Bacteriology, Central Institute for Nutrition and Food Research T.N.O., Utrechtseweg 48, Zeist
E. H. Kampelmacher
Affiliation:
Laboratory for Zoonoses, National Institute of Public Health, Sterrenbos 1, Utrecht
L. M. V. Noorle Jansen
Affiliation:
Laboratory for Zoonoses, National Institute of Public Health, Sterrenbos 1, Utrecht
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Summary

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An investigation was carried out on the influence of the mode of transport at ca. 21° C. on the fate of S. typhimurium, E. coli, Ps. aeruginosa, Staph. aureus, Str. pyogenes and spores of B. cereus collected from surfaces by swabbing with cotton and alginate.

Transport at relative humidity 0·58–0·95 leads to losses in viable counts sometimes exceeding two log cycles. In Stuart's medium and Ringer-calgon particularly Enterobacteriaceae showed growth when transported at 21° C. Reduction of the pH value of Stuart's medium led to losses of far over one log cycle in some types of bacteria and could therefore not be recommended.

Slight chilling of the medium, e.g. to 12° C., and insulation during transport appeared to maintain counts of the organisms studied at their original level in both Stuart's medium and Ringer calgon for 10–12 hr.

Type
Research Article
Information
Journal of Hygiene , Volume 66 , Issue 1 , March 1968 , pp. 59 - 66
Copyright
Copyright © Cambridge University Press 1968

References

REFERENCES

Dernby, K. G. (1921). La concentration optima en ions hydrogène favorisant le développement de certains micro-organismes. Annls Inst. Pasteur, Paris 35, 277–90.Google Scholar
Ellner, P. D. & Ellner, C. J. (1966). Survival of bacteria on swabs. J. Bact. 91, 905–6.CrossRefGoogle ScholarPubMed
Higgins, M. (1950). A comparison of the recovery rate of organisms from cotton-wool and calcium alginate wool swabs. Mon. Bull. Minist. Hlth 9, 50–1.Google Scholar
Knaysi, G. (1951). Elements of Bacterial Cytology, pp. 247–51. Ithaca.Google Scholar
Legault, R. R., Makower, B. & Talburt, W. F. (1948). Apparatus for measurement of vapor pressure. Analyt. Chem. 20, 428–30.CrossRefGoogle Scholar
Mossel, D. A. A. (1967). Ecological principles and methodological aspects of the examination of foods and feeds for indicator micro-organisms. J. Ass. off. Analyt. Chem. 50, 91–104.Google Scholar
Mossel, D. A. A. & Büchli, K. (1964). The total object swab (‘TOS’) technique. A reliable and convenient method for the examination of some proteinaceous staple foods for various types of Enterobacteriaceae. Lab. Pract. 13, 1184–7.Google Scholar
Mossel, D. A. A., Kampelmacher, E. H. & Noorle, Jansen L. M. VAN (1966). Verification of adequate sanitation of wooden surfaces used in meat and poultry processing. Zentbl. Bakt. ParasitKde Abt. 1, Orig. 201, 91–104.Google Scholar
Mossel, D. A. A. & Koopman, M. J. (1965). Losses in viable cells of Salmonellae upon inoculation into dry animal feeds of various types. Poult. Sci. 44, 890–2.Google Scholar
O'beien, F. E. M. (1948). The control of humidity by saturated salt solutions. J. scient. Instrum. 25, 73–6.Google Scholar
Scott, W. J. (1957). Water relations of food spoilage micro-organisms. Adv. Fd. Res. 7 83–127.CrossRefGoogle Scholar
Stokes, R. H. & Robinson, R. A. (1949). Standard solutions for humidity control at 25° C. Ind. Engng Chem. 41, 2013.Google Scholar
Straka, R. P. & Stokes, J. L. (1957). Rapid destruction of bacteria in commonly used diluents and its elimination. Appl. Microbiol. 5, 21–5.CrossRefGoogle ScholarPubMed
Stuart, R. D. (1959). Transport medium for specimens in Public Health bacteriology. Publ. Hlth. Rep., Wash. 74, 431–8.Google Scholar