Hostname: page-component-586b7cd67f-dsjbd Total loading time: 0 Render date: 2024-11-24T23:42:12.609Z Has data issue: false hasContentIssue false

Occurrence of H-antigen z66 of R phase in cultures of Salmonella serovar typhi originated from Indonesia

Published online by Cambridge University Press:  15 May 2009

K Tamura
Affiliation:
National Institute of Health, Kamiosaki, Shinagawa-ku, Tokyo 141, Japan
R Sakazaki
Affiliation:
National Institute of Health, Kamiosaki, Shinagawa-ku, Tokyo 141, Japan
S Kuramochi
Affiliation:
National Institute of Health, Kamiosaki, Shinagawa-ku, Tokyo 141, Japan
A Nakamura
Affiliation:
National Institute of Health, Kamiosaki, Shinagawa-ku, Tokyo 141, Japan
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Eighteen strains of Salmonella choleraesuis subsp. choleraesuis serovar Typhi (S. typhi) isolated from blood of patiens in Japan who had visited Indonesia and returned before the onset of typhoid fever were found to possess the H-antigen z66reported by Guinée et al. (1981) but had the naturally occuring H-antigen d. They were not lysed by any of the phages of the Vi phage typing system. After passage through semi-solid medium containing H-z66 antiserum, H-antigens of 11 of 18 cultures with H-z66 phage changed to H-j and those of 2 others to H-d, while the remaining 5 were immobilized. With cultures in the H-d or H-j phages, change of the H-antigens did not occur when they were cultured in semi-solid medium containing homologous H-antiserum. These phase induction experiments as well as colony examination of original cultures suggest that H-z66 phase is unstable and tends to change of H-antigen to H-d or H-j occured without difficulty probably represented a mixed population of cells in the H-z66 and H-d or H-j phases. Since none of 856 isolates from Japan or fromimported cases of typhoid fever from Southeast Asia other than Indonesia exhibited the H-z66 antigen, it was concluded that the focus of typhois fever caused by S. typhi in H–z66 phase was probably in Indonesia.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1988

References

Edwards, P. R. & Ewing, W. H. (1972). Identification of Enterobacteriaceae, 3rd ed.Minneapolis: Burgess.Google Scholar
Guinée, P. A. M., Jansen, W. H., Maass, H. M. E., le Minor, L. & Beaud, R. (1981). An unusual H antigen (z66) in strains of Salmonella typhi. Annales de Microbiologie (Institut Pasteur) 132 A, 331334.Google ScholarPubMed
Kauffmann, F. (1936). Über die diphasische Natur der Typhus-bacillen. Zeitschrift für Hygiene 119, 103118.CrossRefGoogle Scholar
Kauffmann, F. (1966). The Bacteriology of Enterobacteriaceae. Copenhagen: Munksgaard.Google Scholar