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Mycoplasmas in cell cultures from rheumatoid synovial membranes

Published online by Cambridge University Press:  15 May 2009

K. B. Fraser ,
P. V. Shirodaria ,
Margaret Haire  and
D. Middleton
K. B. Fraser
Affiliation:
Department of Microbiology, The Queen's University of Belfast, Grosvenor Rood, Belfast, BT12 6BN
P. V. Shirodaria
Affiliation:
Department of Microbiology, The Queen's University of Belfast, Grosvenor Rood, Belfast, BT12 6BN
Margaret Haire
Affiliation:
Department of Microbiology, The Queen's University of Belfast, Grosvenor Rood, Belfast, BT12 6BN
D. Middleton
Affiliation:
Department of Microbiology, The Queen's University of Belfast, Grosvenor Rood, Belfast, BT12 6BN
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Summary

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Ten strains of myocoplasmas were recovered from cultures of synovium or cultures inoculated with synovial fragments from rheumatoid arthritis and one from osteo-arthritis. The source of the organisms is not known. Patients with rheumatoid arthritis had no complement-fixing antibody and no fluorescent staining antibody against the mycoplasmas isolated and no mycoplasma antigen was detected by immunofluorescence in sections of synovia and in synovial fluids.

The strains isolated were of two main serological types and could be distinguished by direct fluorescent antibody staining from standard types of human commensals and the common tissue-culture contaminants. One may be Mycoplasma laidlawii.

Type
Research Article
Information
Journal of Hygiene , Volume 69 , Issue 1 , March 1971 , pp. 17 - 25
Copyright
Copyright © Cambridge University Press 1971

References

REFERENCES

Bartholomew, L. E. (1965). Isolation and characterization of mycoplasmas (PPLO) from patients with rheumatoid arthritis, systemic lupus erythematosis and Reiter's syndrome. Arthritis and Rheumatism 8, 376.CrossRefGoogle ScholarPubMed
Clyde, W. A. (1964). Mycoplasma species identification based upon growth inhibition by specific antisera. Journal of Immunology 92, 958.CrossRefGoogle ScholarPubMed
Duthie, J. J. R., Stewart, S. M., Alexander, W. R. M. & Dayhoff, R. E. (1967). Isolation of diphtheroid organisms from rheumatoid synovial membrane and fluid. Lancet i, 142.CrossRefGoogle Scholar
Editorial (1967). Focal and local infection in rheumatoid arthritis. Lancet i, 1372.Google Scholar
Hutchinson, D. (1969). Modified medium for isolation of mycoplasmas from contaminated material. Journal of Medical Laboratory Technology 26, 111.Google ScholarPubMed
Macpherson, I. (1966). Mycoplasmas in tissue culture. Journal of Cell Science 1, 145.CrossRefGoogle Scholar
Macpherson, I. & Montagnier, L. (1964). Agar suspension culture for the selective assay of cells transformed by polyoma virus. Virology 23, 291.CrossRefGoogle ScholarPubMed
Macpherson, I. & Stoker, M. (1962). Polyoma transformation of hamster cell clones - an investigation of genetic factors affecting cell competence. Virology 16, 147.CrossRefGoogle ScholarPubMed
Marmion, B. P. (1967). The mycoplasmas. In Recent Advances in Medical Research, p. 173. Ed. Waterson, A. P.. London: J. and A. Churchill Ltd.Google Scholar
Pease, P. (1969). Bacterial L-forms in the blood and joint fluids of arthritic subjects. Annals of the Rheumatic Diseases 28, 270.CrossRefGoogle ScholarPubMed
Rinderknecht, H. (1962). Ultra-rapid fluorescent labelling of proteins. Nature, London 193, 167.CrossRefGoogle ScholarPubMed
Taylor-Robinson, D., Ludwig, W. M., Purcell, R. H., Mufson, M. A. & Chanock, R. M. (1965). Significance of antibody of Mycoplasma hominis, Type 1, as measured by indirect haemagglutination. Proceedings of the Society for Experimental Biology and Medicine 118, 1073.CrossRefGoogle Scholar
Zgorniak-Nowosielska, I., Sedwick, W. D., Hummeler, K. & Koprowski, H. (1967). New assay procedure for separation of mycoplasmas from virus pools and tissue culture systems. Journal of Virology 1, 1227.CrossRefGoogle ScholarPubMed