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Mycoplasmacidal activity of bovine milk for T-mycoplasmas

Published online by Cambridge University Press:  15 May 2009

J. Brownlie ,
C. J. Howard  and
R. N. Gourlay
J. Brownlie
Affiliation:
Agricultural Research Council, Institute for Research on Animal Diseases, Compton, Newbury, Berks. RG16 0NN
C. J. Howard
Affiliation:
Agricultural Research Council, Institute for Research on Animal Diseases, Compton, Newbury, Berks. RG16 0NN
R. N. Gourlay
Affiliation:
Agricultural Research Council, Institute for Research on Animal Diseases, Compton, Newbury, Berks. RG16 0NN
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Summary

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Normal bovine milk and whey was mycoplasmacidal for 6 of the 13 strains of bovine T-mycoplasmas examined. The in vitro assay used also demonstrated no killing of the human, canine and simian T-mycoplasma strains after 4 hr. incubation. However, there appeared to be some cow-to-cow variation in possession of this activity, and following E. coli endotoxin stimulation of the mammary gland the activity was considerably reduced.

Whey from three normal cows was fractionated on a Bio-Gel A 1·5 m. column and the mycoplasmacidal activity of the resulting five peaks assayed. Only the second peak, peak B, contained activity and was characterized as the only peak containing bovine IgA. The active component in whey, however, was found to be heat stable at 60° C. for 60 minutes and to pass through a dialysis membrane. This is inconsistent with it being immunoglobulin.

Type
Research Article
Information
Journal of Hygiene , Volume 73 , Issue 3 , December 1974 , pp. 415 - 423
Copyright
Copyright © Cambridge University Press 1974

References

REFERENCES

Black, F. T. (1973). Modifications of the Growth Inhibition Test and its application to human T-mycoplasmas. Applied Microbiology 25, 528–33.CrossRefGoogle ScholarPubMed
Broadhurst, J. & Paley, C. (1939). A single dip stain for the direct examination of milk. Journal of the American Veterinary Medical Association 94, 525.Google Scholar
Brownlie, J. (1972). The isolation and characterization of antimicrobial proteins from bovine milk. Ph.D. Thesis, University of Reading.Google Scholar
Brownlie, J. & Hibbitt, K. G. (1972). Antimicrobial proteins isolated from cervical mucus. Journal of Reproduction and Fertility 29, 337–47.CrossRefGoogle ScholarPubMed
Cole, B. C. & Ward, J. R. (1973). Interaction of Mycoplasma arthritidis and other mycoplasmas with murine peritoneal macrophages. Infection and Immunity 7, 691–9.CrossRefGoogle ScholarPubMed
Dajani, A. S. & Ayoub, E. M. (1969). Mycoplasmacidal effects of polymorphonuclear leukocyte extract. Journal of Immunology 102, 698–701.Google Scholar
Derbyshire, J. B. (1964). The multiplication of Staphylococcus aureus in the milk of cows with mild mastitis. Journal of Pathology and Bacteriology 87, 137–44.CrossRefGoogle ScholarPubMed
Gourlay, R. N., Brownlie, J. & Howard, C. J. (1973). Isolation of T-mycoplasmas from goats, and the production of sub-clinical mastitis in goats by the intramammary inoculation of human T-mycoplasmas. Journal of General Microbiology 76, 251–4.CrossRefGoogle Scholar
Gourlay, R. N., Howard, C. J. & Brownlie, J. (1972). The production of mastitis in cows by the intramammary inoculation of T-mycoplasmas. Journal of Hygiene 70, 511–21.CrossRefGoogle ScholarPubMed
Hanssen, F. S. (1924). The bactericidal property of milk. British Journal of Experimental Pathology 5, 271–80.Google Scholar
Hesse, W. (1934). Ueber die Beziehung e zwischen Kuhmilch und Cholerabacillen. Zeitschrift für Hygiene und Infektionskrankheiten 17, 239–71.Google Scholar
Hirsch, J. G. (1960). Antimicrobial factors in tissue and phagocytic cells. Bacteriological Reviews 24, 133–40.CrossRefGoogle ScholarPubMed
Howard, C. J., Gourley, R. N. & Brownlie, J. (1973). The virulence of T-mycoplasmas isolated from various animal species, assayed by intramammary inoculation in cattle. Journal of Hygiene 71, 163–70.CrossRefGoogle ScholarPubMed
Jones, I. G. & Hirsch, J. G. (1971). The interaction in vitro of Mycoplasma pulmonis with mouse peritoneal macrophages and L-cells. Journal of Experimental Medicine 133, 231–59.Google Scholar
Lloyd, L. C. & Trethewie, E. R. (1970). In The Role of Mycoplasmas and L-forms of Bacteria in Diseases(ed. Sharp, J. T.). Springfield, Illinois: Charles C. Thomas.Google Scholar
Oram, J. D. & Reiter, B. (1968). Inhibition of bacteria by lactoferrin and other iron chelating agents. Biochimica et biophysica acta 170, 351–65.CrossRefGoogle ScholarPubMed
Pattison, I. H. & Holman, H. H. (1951). Studies on experiments with streptococcal mastitis. Journal of Comparative Pathology 61, 26–37.Google Scholar
Priestley, F. W. (1952). Observations on immunity to contagious bovine pleuropneumonia with special reference to the bactericidal action of blood. British Veterinary Journal 108, 153–61.Google Scholar
Shah, P. C. & Morse, E. (1964). Studies on albumin in ‘mastitic’ milk: factors affecting the presence of blood-serum albumin in ‘mastitic’ milk. American Journal of Veterinary Research 25, 714–19.Google ScholarPubMed
Simberkoff, M. S. & Elsbach, P. (1971). The interaction in vitro between polymorphonuclear leukocytes and mycoplasma. Journal of Experimental Medicine 134, 1417–30.Google Scholar
Taylor-Robinson, D., Thomas, M. & Dawson, P. C. (1969). The isolation of T-mycoplasmas from the urogenital tract of bulls. Journal of Medical Microbiology 2, 527–33.Google Scholar
Tully, J. G. & Rask-Nielsen, R. (1967). Mycoplasma in leukemic and non-leukemic mice. Annals of the New York Academy of Sciences 143, 345–52.CrossRefGoogle Scholar
Wilson, A. T. & Rosenblum, H. (1952). The antistreptococcal property of milk – the effect of lactenin on haemolytic streptococci of several serological groups. Journal of Experimental Medicine 95, 25–38.Google Scholar
Zucker-Franklin, D., Davidson, M. & Thomas, L. (1966). The interaction of mycoplasmas with mammalian cells, HeLa cells, neutrophils and eosinophils. Journal of Experimental Medicine 124, 521–42.CrossRefGoogle ScholarPubMed