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Multiply- and methicillin-resistant Staphylococcus aureus strains isolated in the German Democratic Republic in 1985 and 1986

Published online by Cambridge University Press:  19 October 2009

W. Witte  and
Chr. Braulke
W. Witte
Affiliation:
Institut für Experimentelle Epidemiologie, Burgstrasse 37, DDR-3700 Wernigerode, German Democratic Republic
Chr. Braulke
Affiliation:
Institut für Experimentelle Epidemiologie, Burgstrasse 37, DDR-3700 Wernigerode, German Democratic Republic
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Summary

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Multiply- and methicillin-resistant Staphylococcus aureus (MRSA) strains have been isolated from five small outbreaks of nosocomial infection in five different hospitals. The MRSA were typed by phage patterns, biochemical traits, resistance phenotypes and plasmid patterns. Three different groups of strains can be distinguished. The MRSA from three outbreaks in one country share identical characters.

Phage typing by the use of the International Basic Set for Phage Typing staphylococci as well as experimental phages does not completely discriminate between the strains. Attribution of several resistance determinants to plasmids in two of the described strain groups proved valuable for strain differentiation.

These multiply-resistant strains are sensitive to vancomycin and to rifampicin.

Type
Research Article
Information
Epidemiology & Infection , Volume 99 , Issue 3 , December 1987 , pp. 603 - 612
Copyright
Copyright © Cambridge University Press 1987

References

REFERENCES

Blair, J. E. & Williams, R. E. O. (1961). Phage-typing of staphylococci. Bulletin of the World Health Organization 24, 771–784.Google ScholarPubMed
Dowd, G., Cafferkey, M. & Dougan, G. (1983). Gentamicin and methicillin resistant Staphylococcus aureus in Dublin hospitals: molecular studies. Journal of Medical Microbiology 16, 129–138.CrossRefGoogle ScholarPubMed
El, Solh N., Alignet, J., Bismuth, R., Buret, B. & Fouace, I.-M. (1986 b). Conjugative transfer of staphylococcal antibiotic resistance markers in the absence of detectable plasmid DNA. Antimicrobial Agents and Chemotherapy 30, 161–169.Google Scholar
El, Sohl N., Moreau, N. & Ehrlich, S. (1986 a). Molecular cloning and analysis of Staphylococcus aureus chromosomal aminoglycoside resistance genes. Plasmid 15, 104–118.Google Scholar
Emslie, K. R., Townsend, D. E. & Grubb, W. B. (1985). A resistance determinant to nucleid acid-binding compounds in methicillin-resistant Staphylococcus aureus. Journal of Medical Microbiology 20, 139–145.CrossRefGoogle Scholar
Gillespie, M. T. & Skurray, R. A. (1986). Plasmids in multiresistant Staphlococcus aureus. Microbiological Sciences 3, 53–58.Google Scholar
Goering, R. V. & Ruff, E. A. (1983). Comparative analysis of conjugative plasmids mediating gentamicin resistance in Staphylococcus auretus. Antimicrobial Agents and Chemotherapy 24, 450–452.CrossRefGoogle Scholar
Haley, R., Hightower, A., Khabbaz, R. F., Thornsbery, C., Martone, W. J., Allen, J. R. & Hughes, J. M. (1982). The emergence of methicillin-resistant Staphylococcus aureus infections in United States hospitals. Annals of International Medicine 97, 297–308.CrossRefGoogle ScholarPubMed
Iordanuscu, S. & Surreanu, M. (1980). New incompatibility groups for Staphlococcus aureus plasmids. Plasmid 4, 256–260.CrossRefGoogle Scholar
Kado, C. I. & Liu, S. T. (1981). Rapid procedure for detection and isolation of large and small plasmids. Journal of Bacteriology 145, 1365–1373.CrossRefGoogle ScholarPubMed
Kayser, F. H. (1980). Die Resistenz methicillinresistenter Staphylokokken gegenüber neuen Cephalosporin-Antibiotika. Infection 8, 165–170.CrossRefGoogle Scholar
Kayser, F. H., Homberger, F. & Devaud, M. (1981). Aminocyclitol-modifying enzymes specified by choromsomal genes in Staphylococctis aureus. Antimicrobial Agents and Chemotherapy 19, 760–772.CrossRefGoogle ScholarPubMed
Locksley, R. M., Cohen, M. L., Quinn, T. C., Tompkins, L. S., Coyle, M. B., Kihara, J. M. & Counts, G. W. (1982). Multiply resistant Staphylococcus aureus: introduction, transmission and evolution of nosocomial infection. Annals of International Medicine 97, 317–324.CrossRefGoogle ScholarPubMed
Lyon, B. R., Iurio, J. L., May, J. W. & Skurray, R. A. (1984). Molecular epidemiology of multiresistant Staphylococcus aureus in Australian hospitals. Journal of Medical Microbiology 17, 79–89.CrossRefGoogle ScholarPubMed
Lyon, B. R., May, J. W. & Skurray, R. A. (1984). In 4001: a gentamicin and kanamycin resistance transposon in Staphylococcus aureus. Molecular and General Genetics 193. 554–556.CrossRefGoogle Scholar
Maniatis, T., Fritsch, E. F. & Sambrook, J. (1982). Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.Google Scholar
Marples, R. R., Richardson, J. & De Saxe, M. (1986). Bacteriological characters of strains of Staphylococcus aureus submitted to a reference laboratory related to methicillin resistance. Journal of Hygiene 96, 217–223.CrossRefGoogle ScholarPubMed
Meyer, W., Ziomek, D. & Rische, H. (1973). Staphylococcus aureus. In Lysotypie und andere spezielle epidemiologische Laboratoriumsmethoden. (ed. Rische, H.), Jena: Gustav Fischer.Google Scholar
Naidoo, J., Noble, W. C., Weissmann, A. & Dyke, K. G. H. (1983). Gentamicin-resistant staphylococci: genetics of an outbreak in a dermatology department. Journal of Hygiene 91, 7–16.CrossRefGoogle Scholar
Novick, R. P. (1967). Properties of cryptic high frequency transducing phage in Staphylococcus aureus. Virology 33, 155–166.CrossRefGoogle ScholarPubMed
Pavillard, R., Harvey, K., Douglas, D., Hewstone, A., Andrew, J., Collopy, B., Asche, V., Carson, P., Davidson, A., Gilbert, G., Spicer, J. & Tosolini, F. (1982). Epidemic of hospital acquired infection due to methicillin-resistant Staphylococcus aureus in major Victorian hospitals. Medical Journal of Australia 1, 451–454.CrossRefGoogle ScholarPubMed
Reissbrodt, R., Witte, W. & Rische, H. (1983). A new semidefined nutrient medium for bacterial susceptibility testing. Journal of Hygiene, Microbiology, Epidemiology and Immunology (Prague) 27, 465–479.Google ScholarPubMed
Rische, H. & Witte, W. (1985). The clone concept in the epidemiology of staphylococal infection. Zentralblatt für Bakteriologie, supplement 14, 531–540.Google Scholar
Ruby, C. & Novick, R. P. (1975). Plasmid interactions in Staphylococcus aureus: nonadditivity of compatible plasmid DNA pools. Proceedings of the National Academy of Sciences, U.S.A. 72, 5031–5035.CrossRefGoogle ScholarPubMed
Thrupp, L. D. (1981). Susceptibility testing of antibiotics in liquid media. In Antibiotics in Laboratory Medicine (ed. Lorian, V.). Baltimore and London: Williams & Wilkins.Google Scholar
Townsend, D. E., Greed, L., Ashdown, N. & Grubb, W. B. (1983). Plasmid mediated resistance to quarternary ammonium compounds in methicillin resistant Staphylococcus aureus. Medical Journal of Australia 2, 310.CrossRefGoogle Scholar
Townsend, D. E., Ashdown, N., Greed, L. C. & Grubb, W. B. (1984). Transposition of gentamicin resistance to staphylococcal plasmids encoding resistance to cationic agents. Journal of Antimicrobial Chemotherapy 14, 115–124.CrossRefGoogle ScholarPubMed
Vickery, A., Beard-Pegler, M. A. & Rountree, P. M. (1983). Strain differentiation in methicillin-resistant Staphylococcus aureus. Pathology 15, 235–240.CrossRefGoogle ScholarPubMed
Witte, W. (1976). Restriktion und Modifikation bei Staphylococcus aureus. Eigenschaften von Resistenzplasmiden und Prophagen. Zeitschrijt für Allgemeine Mikrobiologie 16, 465–474.Google Scholar
Witte, W. & Dünnhaupt, K. (1984). Occurrence of a nonplasmid-located determinant for gentamicin-resistance in strains of Staphylococcus aureus. Journal of Hygiene 93, 1–8.CrossRefGoogle ScholarPubMed
Witte, W., Green, L., Misra, T. & Silver, S. (1986). Resistance to mercury and to cadmium in chromosomally resistant Staphylococcus aureus. Antimicrobial Agents and Chemotherapy 29, 663–669.CrossRefGoogle ScholarPubMed
Witte, W., Dip, Nguyen van & Dünnhaupt, K. (1986). Methicillin-resistant Staphylococcus aureus (MRSA) in the German Democratic Republic, incidence and strain characteristics. Journal of Hygiene, Microbiology, Epidemiology and Immunology (Prague) 30, 185–193.Google ScholarPubMed
Wyman, L., Goering, R. V. & Novick, R. P. (1974). Genetics of chromosomal and plasmid recombination in Staphylococcus aureus. Genetics 76, 681–702.CrossRefGoogle ScholarPubMed