Molecular typing of Escherichia coli O157[ratio ]H7 (H−) isolates from cattle in Japan

M. AKIBA; T. MASUDA; T. SAMESHIMA; K. KATSUDA; M. NAKAZAWA

doi:10.1017/S0950268899002198

Abstract

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A total of 77 Escherichia coli O157[ratio ]H7 (H−) isolates from cattle in Japan were investigated by molecular biological methods. Most of these isolates (43 isolates) possessed the stx2 gene, but not stx1. Fifteen bacteriophage types and 50 pulsed-field gel electrophoresis (PFGE) profiles were observed. One isolate was indistinguishable from the human outbreak strain by these methods. This indicates that cattle must be considered as a possible source of human E. coli O157[ratio ]H7 infection in Japan.

Crossref Citations

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Nakazawa, Muneo Akiba, Masato and Sameshima, Toshiya 1999. Swine as a Potential Reservoir of Shiga Toxin-Producing Escherichiacoli O157:H7 in Japan. Emerging Infectious Diseases, Vol. 5, Issue. 6, p. 833.

Misawa, Naoaki Sueyoshi, Masuo Uemura, Ryoko Kakemizu, Yoshihiro Kawashima, Kumiko Nagatomo, Hiroshi Kondo, Fusao Murakami, Takayuki and Takahashi, Yoshihiro 2000. Effect of Bicozamycin on the Eradication of Shiga Toxin‐Producing Escherichia coli in Calves. Microbiology and Immunology, Vol. 44, Issue. 11, p. 891.

Akiba, Masato Sameshima, Toshiya and Nakazawa, Muneo 2000. Clonal turnover of enterohemorrhagicEscherichia coliO157:H7 in experimentally infected cattle. FEMS Microbiology Letters, Vol. 184, Issue. 1, p. 79.

Terajima, J. Izumiya, H. Wada, A. Tamura, K. and Watanabe, H. 2000. Molecular epidemiological investigation of enterohaemorrhagicEscherichia coliisolates in Japan. Journal of Applied Microbiology, Vol. 88, Issue. S1, p. 99S.

AGUI, Noriaki 2001. Flies carrying enterohemorrhagic Escherichia coli (EHEC) O157 in Japan : A nationwide survey. Medical Entomology and Zoology, Vol. 52, Issue. 2, p. 97.

Barkocy-Gallagher, Genevieve A. Arthur, Terrance M. Siragusa, Gregory R. Keen, James E. Elder, Robert O. Laegreid, William W. and Koohmaraie, Mohammad 2001. Genotypic Analyses of Escherichia coli O157:H7 and O157 Nonmotile Isolates Recovered from Beef Cattle and Carcasses at Processing Plants in the Midwestern States of the United States . Applied and Environmental Microbiology, Vol. 67, Issue. 9, p. 3810.

Mora, Azucena Blanco, Miguel Blanco, Jesús E. Alonso, M. Pilar Dhabi, Ghizlane Thomson-Carter, Fiona Usera, Miguel A. Bartolomé, Rosa Prats, Guillermo and Blanco, Jorge 2004. Phage Types and Genotypes of Shiga Toxin-Producing Escherichia coli O157:H7 Isolates from Humans and Animals in Spain: Identification and Characterization of Two Predominating Phage Types (PT2 and PT8) . Journal of Clinical Microbiology, Vol. 42, Issue. 9, p. 4007.

Adwan, Ghaleb M. and Adwan, Kamel M. 2004. Isolation of shiga toxigenic Escherichia coli from raw beef in Palestine. International Journal of Food Microbiology, Vol. 97, Issue. 1, p. 81.

Vali, Leila Wisely, Karen A. Pearce, Michael C. Turner, Esther J. Knight, Hazel I. Smith, Alastair W. and Amyes, Sebastian G. B. 2004. High-Level Genotypic Variation and Antibiotic Sensitivity among Escherichia coli O157 Strains Isolated from Two Scottish Beef Cattle Farms . Applied and Environmental Microbiology, Vol. 70, Issue. 10, p. 5947.

Sekse, Camilla Solberg, Asta Petersen, Anne Rudi, Knut and Wasteson, Yngvild 2005. Detection and quantification of Shiga toxin-encoding genes in sheep faeces by real-time PCR. Molecular and Cellular Probes, Vol. 19, Issue. 5, p. 363.

Avery, S.M. Walters, L.D. and Hutchison, M.L. 2005. Fate of Escherichia coli O157 and detection of stx phage during fermentation of maize, an animal feedstuff. Letters in Applied Microbiology, Vol. 40, Issue. 2, p. 99.

Vali, L. Hamouda, A. Pearce, M.C. Knight, H.I. Evans, J. and Amyes, S.G.B. 2007. Detection of genetic diversity by pulsed-field gel electrophoresis among Escherichia coli O157 isolated from bovine faecal samples by immunomagnetic separation technique. Letters in Applied Microbiology, Vol. 44, Issue. 1, p. 19.

Regua-Mangia, A.H. C. Andrade, J.R. M. Gonzalez, A.G. Zahner, V. F. Cerqueira, A.M. and Teixeira, L.M. 2008. Genetic relatedness of a non-motile variant O157 enteropathogenic Escherichia coli (EPEC) strain and E. coli strains belonging to pathogenic related groups. Microbiological Research, Vol. 163, Issue. 2, p. 225.

Yoshii, Noriyo Ogura, Yoshitoshi Hayashi, Tetsuya Ajiro, Takashi Sameshima, Toshiya Nakazawa, Muneo Kusumoto, Masahiro Iwata, Taketoshi and Akiba, Masato 2009. Pulsed-Field Gel Electrophoresis Profile Changes Resulting from Spontaneous Chromosomal Deletions in Enterohemorrhagic Escherichia coli O157:H7 during Passage in Cattle . Applied and Environmental Microbiology, Vol. 75, Issue. 17, p. 5719.

Kawano, K. Ono, H. Iwashita, O. Kurogi, M. Haga, T. Maeda, K. and Goto, Y. 2012. stx genotype and molecular epidemiological analyses of Shiga toxin-producing Escherichia coli O157:H7/H− in human and cattle isolates. European Journal of Clinical Microbiology & Infectious Diseases, Vol. 31, Issue. 2, p. 119.

Sadiq, Shah M. Hazen, Tracy H. Rasko, David A. Eppinger, Mark Sperandio, Vanessa and Hovde, Carolyn J. 2014. EHEC Genomics: Past, Present, and Future. Microbiology Spectrum, Vol. 2, Issue. 4,

Sadiq, Shah M. Hazen, Tracy H. Rasko, David A. and Eppinger, Mark 2015. EnterohemorrhagicEscherichia coliand Other Shiga Toxin-ProducingE. coli. p. 55.

Eppinger, Mark and Cebula, Thomas A 2015. Future perspectives, applications and challenges of genomic epidemiology studies for food-borne pathogens: A case study of EnterohemorrhagicEscherichia coli(EHEC) of the O157:H7 serotype. Gut Microbes, Vol. 6, Issue. 3, p. 194.

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Molecular typing of Escherichia coli O157[ratio ]H7 (H−) isolates from cattle in Japan

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