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Molecular typing of Acinetobacter baumannii–Acinetobacter calcoaceticus complex isolates from endemic and epidemic nosocomial infections

Published online by Cambridge University Press:  15 May 2009

P. Ratto ,
D. O. Sordelli ,
E. Abeleira ,
M. Torrero  and
M. Catalano
P. Ratto
Affiliation:
Departamento de Microbiología, Facultad de Medicina, Universidad de Buenos Aires, Argentina
D. O. Sordelli
Affiliation:
Departamento de Microbiología, Facultad de Medicina, Universidad de Buenos Aires, Argentina
E. Abeleira
Affiliation:
Departamento de Microbiología, Facultad de Medicina, Universidad de Buenos Aires, Argentina
M. Torrero
Affiliation:
Departamento de Microbiología, Facultad de Medicina, Universidad de Buenos Aires, Argentina
M. Catalano*
Affiliation:
Departamento de Microbiología, Facultad de Medicina, Universidad de Buenos Aires, Argentina
*
*Mariana Catalo, Departamento de Microbiología FCM-UBA, Paraguay 2155 P-12, (1121) Buenos Aires, Argentina.
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Ribotype, biotype and resistance phenotype were used to characterize 37 Acinetobacter baumannii–A. calcoaceticus complex isolates responsible for nosocomial infections in Buenos Aires. Nineteen isolates were recovered from endemic infections at 2 hospitals and 18 represent an intensive care unit outbreak that occurred in a third hospital. By ribotyping isolates were classified into five different clones of A. baumannii biotype 2, 3 of A. baumannii biotype 9, and 3 of Acinetobacter genospecies 13. Combination of the three epidemiological markers permitted categorization of 18 outbreak isolates into four probable strains: 2 A. baumannii biotype 2. named type I. and II, and 2 A. baumannii biotype 9. Type I (15 isolates) was the most prevalent strain at one hospital and was responsible for the outbreak. In conclusion, combined analysis of biotypes, resistance phenotypes, and ribotypes was an accurate approach for epidemiologic investigation of A. baumannii. Furthermore, ribotyping discriminated Acinetobacter genospecies 13 isolates which were phenotypically difficult to type.

Type
Special Article
Information
Epidemiology & Infection , Volume 114 , Issue 1 , February 1995 , pp. 123 - 132
Copyright
Copyright © Cambridge University Press 1995

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