Molecular typing methods to investigate transmission of Escherichia coli O157[ratio ]H7 from cattle to humans

M. LOUIE; S. READ; L. LOUIE; K. ZIEBELL; K. RAHN; A. BORCZYK; H. LIOR

doi:10.1017/S0950268899002551

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The utility of phage typing, pulsed-field gel electrophoresis (PFGE), and plasmid profile analysis was compared, to differentiate between Canadian Escherichia coli O157[ratio ]H7 strains of human (n = 27) and cattle (n = 24) origin. The diversity indices for phage typing, plasmid analysis and PFGE were 0·85, 0·69 and 0·93, respectively. PFGE and phage typing were also applied to study the role of direct transmission of E. coli O157[ratio ]H7 from cattle to humans on isolates collected from two separate farm outbreaks. PFGE showed that more than one E. coli O157[ratio ]H7 strain with varying PFGE DNA subtype profiles, may be responsible for an outbreak, and that more than one E. coli O157[ratio ]H7 subtype may be circulating on a particular farm at any one time. To our knowledge, this is one of the first reports where PFGE typing was used to verify the direct transmission of E. coli O157[ratio ]H7 from cattle to humans.

Crossref Citations

This article has been cited by the following publications. This list is generated based on data provided by Crossref.

Roe, Andrew J. and Gally, David L. 2000. Enteropathogenic and enterohaemorrhagic Escherichia coli and diarrhoea. Current Opinion in Infectious Diseases, Vol. 13, Issue. 5, p. 511.

Enriquez, Carlos Nwachuku, N. and Gerba, C.P. 2001. Direct Exposure to Animal Enteric Pathogens. Reviews on Environmental Health, Vol. 16, Issue. 2,

Proctor, Mary E. Kurzynski, Terry Koschmann, Cindy Archer, John R. and Davis, Jeffrey P. 2002. Four Strains of Escherichia coli O157:H7 Isolated from Patients during an Outbreak of Disease Associated with Ground Beef: Importance of Evaluating Multiple Colonies from an Outbreak-Associated Product . Journal of Clinical Microbiology, Vol. 40, Issue. 4, p. 1530.

Avery, S. M. Liebana, E. Reid, C.-A. Woodward, M. J. and Buncic, S. 2002. Combined Use of Two Genetic Fingerprinting Methods, Pulsed-Field Gel Electrophoresis and Ribotyping, for Characterization of Escherichia coli O157 Isolates from Food Animals, Retail Meats, and Cases of Human Disease . Journal of Clinical Microbiology, Vol. 40, Issue. 8, p. 2806.

Beutin, Lothar Kaulfuss, Stefan Cheasty, Thomas Brandenburg, B.örries Zimmermann, Sonja Gleier, Kerstin Willshaw, Geraldine A and Smith, Henry R 2002. Characteristics and association with disease of two major subclones of Shiga toxin (Verocytotoxin)-producing strains of Escherichia coli (STEC) O157 that are present among isolates from patients in Germany. Diagnostic Microbiology and Infectious Disease, Vol. 44, Issue. 4, p. 337.

Davis, Margaret A. Hancock, Dale D. Besser, Thomas E. and Call, Douglas R. 2003. Evaluation of Pulsed-Field Gel Electrophoresis as a Tool for Determining the Degree of Genetic Relatedness between Strains of Escherichia coli O157:H7 . Journal of Clinical Microbiology, Vol. 41, Issue. 5, p. 1843.

Albihn, A. Eriksson, E. Wallen, C. and Aspán, A. 2003. Verotoxinogenic Escherichia coli(VTEC) O157:H7 – A Nationwide Swedish Survey of Bovine Faeces. Acta Veterinaria Scandinavica, Vol. 44, Issue. 1,

Sisti, Maurizio Benedetti, Claudio Lonzi, Anna Schiavano, Giuditta F. Pianetti, Anna Romanini, Ivana and Bruscolini, Francesca 2004. Isolation of Escherichia coli O157 from human and bovine faeces in the Urbino area, Italy. International Journal of Hygiene and Environmental Health, Vol. 207, Issue. 6, p. 577.

Blanco, Jorge Blanco, Miguel Blanco, Jesus E. Mora, Azucena Alonso, María Pilar Gonzalez, Enrique A. and Bernardez, María Isabel 2004. Verocytotoxigenic E. coli. p. 113.

LeJeune, J. T. Besser, T. E. Rice, D. H. Berg, J. L. Stilborn, R. P. and Hancock, D. D. 2004. Longitudinal Study of Fecal Shedding of Escherichia coli O157:H7 in Feedlot Cattle: Predominance and Persistence of Specific Clonal Types despite Massive Cattle Population Turnover . Applied and Environmental Microbiology, Vol. 70, Issue. 1, p. 377.

Dunn, John R. Keen, James E. and Thompson, R. Alex 2004. Prevalence of shiga-toxigenic Escherichia coli O157:H7 in adult dairy cattle. Journal of the American Veterinary Medical Association, Vol. 224, Issue. 7, p. 1151.

Avery, S.M. Liebana, E. Hutchison, M.L. and Buncic, S. 2004. Pulsed field gel electrophoresis of related Escherichia coli O157 isolates associated with beef cattle and comparison with unrelated isolates from animals, meats and humans. International Journal of Food Microbiology, Vol. 92, Issue. 2, p. 161.

Naylor, Stuart W. Gally, David L. and Christopher Low, J. 2005. Enterohaemorrhagic E. coli in veterinary medicine. International Journal of Medical Microbiology, Vol. 295, Issue. 6-7, p. 419.

Duffy, G. O'Brien, S.B. Carney, E. Sheridan, J.J. McDowell, D.A. and Blair, I.S. 2005. Characterisation of E. coli O157 isolates from bovine hide and beef trimming in Irish abattoirs by pulsed field gel electrophoresis. Journal of Microbiological Methods, Vol. 60, Issue. 3, p. 375.

Tankson, J. D. Fedorka-Cray, P. J. Jackson, C. R. and Headrick, M. 2006. Genetic relatedness of a rarely isolated Salmonella: Salmonella enterica serotype Niakhar from NARMS animal isolates. Journal of Antimicrobial Chemotherapy, Vol. 57, Issue. 2, p. 190.

Low, Alison S. Holden, Nicola Rosser, Tracy Roe, Andrew J. Constantinidou, Chrystala Hobman, Jon L. Smith, David G. E. Low, J. Christopher and Gally, David L. 2006. Analysis of fimbrial gene clusters and their expression in enterohaemorrhagic Escherichia coli O157:H7. Environmental Microbiology, Vol. 8, Issue. 6, p. 1033.

Wells, Timothy J. Sherlock, Orla Rivas, Lucy Mahajan, Arvind Beatson, Scott A. Torpdahl, Mia Webb, Richard I. Allsopp, Luke P. Gobius, Kari S. Gally, David L. and Schembri, Mark A. 2008. EhaA is a novel autotransporter protein of enterohemorrhagic Escherichia coli O157:H7 that contributes to adhesion and biofilm formation. Environmental Microbiology, Vol. 10, Issue. 3, p. 589.

Regua-Mangia, A.H. C. Andrade, J.R. M. Gonzalez, A.G. Zahner, V. F. Cerqueira, A.M. and Teixeira, L.M. 2008. Genetic relatedness of a non-motile variant O157 enteropathogenic Escherichia coli (EPEC) strain and E. coli strains belonging to pathogenic related groups. Microbiological Research, Vol. 163, Issue. 2, p. 225.

David, Donna E. Lynne, Aaron M. Han, Jing and Foley, Steven L. 2010. Evaluation of Virulence Factor Profiling in the Characterization of Veterinary Escherichia coli Isolates . Applied and Environmental Microbiology, Vol. 76, Issue. 22, p. 7509.

Kawano, K. Ono, H. Iwashita, O. Kurogi, M. Haga, T. Maeda, K. and Goto, Y. 2012. stx genotype and molecular epidemiological analyses of Shiga toxin-producing Escherichia coli O157:H7/H− in human and cattle isolates. European Journal of Clinical Microbiology & Infectious Diseases, Vol. 31, Issue. 2, p. 119.

Download full list

Article contents

Molecular typing methods to investigate transmission of Escherichia coli O157[ratio ]H7 from cattle to humans

Abstract

Save article to Kindle

Save article to Dropbox

Save article to Google Drive

Reply to: Submit a response

Your details

You have entered the maximum number of contributors

Conflicting interests