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Molecular relationship among Salmonella dublin isolates identified at the Center for Enterobacteriaceae of Palermo during the years 1971–85

Published online by Cambridge University Press:  19 October 2009

A. Nastasi ,
M. R. Villafrate ,
C. Mammina ,
M. F. Massenti ,
D. Oliva  and
G. Scarlata
A. Nastasi
Affiliation:
Institute of Hygiene ‘G. D'Alessandro’, Center for Enterobacteriaceae of Southern Italy, University of Palermo, via del Vespro 133, 90127 Palermo, Italy
M. R. Villafrate
Affiliation:
Institute of Hygiene ‘G. D'Alessandro’, Center for Enterobacteriaceae of Southern Italy, University of Palermo, via del Vespro 133, 90127 Palermo, Italy
C. Mammina
Affiliation:
Institute of Hygiene ‘G. D'Alessandro’, Center for Enterobacteriaceae of Southern Italy, University of Palermo, via del Vespro 133, 90127 Palermo, Italy
M. F. Massenti
Affiliation:
Institute of Hygiene ‘G. D'Alessandro’, Center for Enterobacteriaceae of Southern Italy, University of Palermo, via del Vespro 133, 90127 Palermo, Italy
D. Oliva
Affiliation:
Institute of Hygiene ‘G. D'Alessandro’, Center for Enterobacteriaceae of Southern Italy, University of Palermo, via del Vespro 133, 90127 Palermo, Italy
G. Scarlata
Affiliation:
Institute of Hygiene ‘G. D'Alessandro’, Center for Enterobacteriaceae of Southern Italy, University of Palermo, via del Vespro 133, 90127 Palermo, Italy
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Summary

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A molecular epidemiological study was carried out on 60 Salmonella dublin isolates identified at the Southern Italy Enterobacteriaceae Center between 1971 and 1985. These included 23 isolates from children with diarrhoea in Palermo obtained during 1984.

All isolates from the outbreak of gastroenteritis in children were resistant to chloramphenicol and streptomycin and harboured two plasmids of 50 MDa and 3 MDa molecular weight, whereas the majority of the isolates identified before 1984 were susceptible to these antibiotics and carried only a 50 MDa molecular weight plasmid. Four S. dublin strains successively identified from cattle (Palermo, Foggia, Portici) and from a child (Palermo) were shown to possess similar antibiotic resistance patterns and plasmid profiles to S. dublin isolates from the outbreak of gastroenteritis in children.

The 50 MDa plasmid was shown to be associated with virulence in mice, while it was not possible to assign any genetic function to the 3 MDa plasmid.

Type
Research Article
Information
Epidemiology & Infection , Volume 99 , Issue 2 , October 1987 , pp. 283 - 290
Copyright
Copyright © Cambridge University Press 1987

References

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