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Molecular epidemiology of the plasmid-encoded TEM-1 β-lactamase in Scotland

Published online by Cambridge University Press:  15 May 2009

C. J. Thomson  and
S. G. B. Amyes
C. J. Thomson
Affiliation:
Scottish Antibiotic Reference Laboratory, Department of Medical Microbiology, The Medical School, University of Edinburgh, Teviot Place, Edinburgh EH8 9AG. Scotland
S. G. B. Amyes
Affiliation:
Scottish Antibiotic Reference Laboratory, Department of Medical Microbiology, The Medical School, University of Edinburgh, Teviot Place, Edinburgh EH8 9AG. Scotland
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A survey of the β-lactamases responsible for ampicillin resistance in urinary Escherichia coli isolated in central Scotland has been performed. The TEM-1 β-lactamase was found to be most prevalent occurring in 88.2% of ampicillin-resistant isolates tested. Forty-six percent of the TEM-1 producing strains were able to transfer this resistance to E. coli J62–2 at 37 ° C. Analysis of the resulting transconjugants revealed that the degree of resistance to amoxycillin and amoxycillin in combination with clavulanic acid was related to the specific activity of the TEM-1 β-lactamase. The variation in specific activity was shown to be related to plasmid type as determined by restriction analysis. No obvious relationship between β-lactamase specific activity and resistance to amoxycillin and amoxycillin plus clavulanic acid could be demonstrated in the original plasmid donor strains.

Type
Research Article
Information
Epidemiology & Infection , Volume 110 , Issue 1 , February 1993 , pp. 117 - 125
Copyright
Copyright © Cambridge University Press 1993

References

REFERENCES

1.Pratt, RF. β-lactamase inhibitors. In: Sandler, M, Smith, HJ. eds. Introduction to the use of enzyme inhibitors as drugs. Oxford: Oxford University Press. 1989: 178–205.Google Scholar
2.Hamilton-Miller, JMT. β-lactamases and their clinical significance. J Antimicrob Chemother 1982: 9 Suppl. B: 11–9.CrossRefGoogle ScholarPubMed
3.Rolinson, GN. Evolution of β-lactamase inhibitors. Rev Infect Dis 1991: 13 Suppl. 9: S727–32.CrossRefGoogle ScholarPubMed
4.Payne, DJ, Amyes, SGB. Transferable resistance to extended-spectrum β-lactams: a major threat or a minor inconvenience? J Antimicrob Chemother 1991: 27: 255–61.CrossRefGoogle ScholarPubMed
5.Philippon, A, Labia, R, Jacoby, G. Extended-spectrum β-lactamases. Antimicrob Agents Chemother 1989: 33: 1131–6.CrossRefGoogle ScholarPubMed
6.Martinez, JL. Cerencendo, E, Rodriquez-Creixems, M. Vincente-Perez, MF. Delgado-Tribarren, A. Baquero, F. Resistance to beta-lactam/clavulanate. Lancet 1987: ii: 1473.CrossRefGoogle Scholar
7.French, G. Ling, J. Amoxycillin/clavulanate resistant Escherichia coli. Lancet 1988: i: 704.CrossRefGoogle Scholar
8.Sanders, CC, Iaconis, JP, Bodey, GD. Samonis, G. Resistance to ticarcillin-potassium clavulanate among clinical isolates of the family Enterobacteriaceae: Role of PSE-1 β-lactamase and high levels of TEM-1 and SHV-1 and problems with false susceptibility in disk diffusion tests. Antimicrob Agents Chemother 1988: 32: 1365–9.CrossRefGoogle ScholarPubMed
9.Shannon, K. Williams, H. King, A. Phillips, I. Hyperproduction of TEM -1 beta-lactamase in clinical isolates of Escherichia coli serotype 015. FEMS Microbiol Lett 1990: 55: 319–23.CrossRefGoogle Scholar
10.Reguera, JA, Baquero, F. Perez-Diaz, JC. Martinez, JL. Factors determining resistance to β-lactam combined with β-lactamase inhibitors in Escherichia coli. J Antimicrob Chemother 1991: 27: 569–75.CrossRefGoogle ScholarPubMed
11. Working Party of the British Society for Antimicrobial Chemotherapy. A guide to sensitivity testing. J Antimicrob Chemother 1991: 27 Suppl D: 1–50.Google Scholar
12.Nandivada, LS. Amyes, SGB. Plasmid-mediated β-lactam resistance in pathogenic gram-negative bacteria isolated in South India. J Antimicrob Chemother 1990: 26: 279–90.CrossRefGoogle ScholarPubMed
13.Rubio, MC. Gil, J. Castillo, J. et al. The susceptibility to amoxycillin/clavulanate of Enterobacteriaceae with plasmid-mediated ampicillin resistance; a twelve-year study of strains in one Spanish hospital. J Antimicrobial Chemother 1989: 24 Suppl B: 35–40.CrossRefGoogle ScholarPubMed
14.Matthew, M. Harris, AM. Marshall, MJ. Ross, GW. The use of analytical isoelectric focusing for detection and identification of β-lactamases. J Gen Microbiol 1975: 88: 169–78.CrossRefGoogle Scholar
15.Reid, AJ. Amyes, SGB. Plasmid penicillin resistance in Vibrio cholerae: identification of a new β-lactamase SAR-1. Antimicrob Agents Chemother 1986: 30: 245–7.CrossRefGoogle ScholarPubMed
16.Waddell, WJ. A simple ultraviolet spectrophotometric method for the determination of protein. J Lab Clin Med 1956: 48: 311–4.Google ScholarPubMed
17.Takahashi, S. Nagano, Y. Rapid procedure for isolation of plasmid DNA and application to epidemiological analysis. J Clin Microbiol 1984: 20: 608–13.CrossRefGoogle ScholarPubMed
18.Huovinen, S. Huovinen, P. Jacoby, G. Detection of plasmid-mediated β-lactamases with DNA probes. Antimicrob Agents Chemother 1988: 32: 175–9.CrossRefGoogle ScholarPubMed
19.Roy, C. Segura, C. Tirado, M. Reig, R. Hermida, M. Tervel, D, Foz, A. Frequency of plasmid determined beta-lactamases in 680 consecutively isolated strains of Enterobacteriaceae. Eur J Clin Microbiol 1986: 4: 146–7.CrossRefGoogle Scholar
20.Simpson, IN. Knothe, H. Plested, SJ. Harper, PB. Qualitative and quantitative aspects of β-lactamase production as mechanisms of β-lactam resistance in a survey of clinical isolates from faecal samples. J Antimicrob Chemother 1986: 17: 725–37.CrossRefGoogle Scholar
21.Thomson, CJ. Amyes, SGB. TRC-1: emergence of a clavulanic acid-resistant TEM β-lactamase in a clinical strain. FEMS Microbiol Lett 1992: 91: 113–8.Google Scholar
22.Jacoby, GA. Sutton, L. Properties of plasmids responsible for production of extended spectrum β-lactamases. Antimicrob Agents Chemother 1991: 35: 164–9.CrossRefGoogle ScholarPubMed
23.Nandivada, LS. Lewin, CS. Amyes, SGB. Bacterial resistance to the combination of ampicillin and clavulanic acid. In Abstracts of the 90th Annual Meeting of the American Society for Microbiology. Anaheim, CA, 1990: Abstract A-87. p. 15. American Society for Microbiology. Washington. DC.Google Scholar
24.Page, JWJ. Farmer, TH. Elson, SW. Hyperproduction of TEM-1 β-lactamase by Escherichia coli strains. J Antimicrob Chemother 1989: 23: 160–1.CrossRefGoogle ScholarPubMed
25.Seetulsingh, PS. Hall, LMC. Livermore, DM. Activity of clavulanate combinations against TEM-1 β-lactamase producing Escherichia coli isolates obtained in 1982 and 1989. J Antimicrob Chemother 1991: 27: 749–59.CrossRefGoogle ScholarPubMed