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Molecular epidemiology of shigella infections in Israel

Published online by Cambridge University Press:  15 May 2009

M. Yavzori ,
D. Cohen  and
H. Bercovier
M. Yavzori
Affiliation:
Israel Defense Force, Medical Corps, Military Post 02149, Israel
D. Cohen
Affiliation:
Israel Defense Force, Medical Corps, Military Post 02149, Israel
H. Bercovier
Affiliation:
Israel Defense Force, Medical Corps, Military Post 02149, Israel Department of Clinical Microbiology, Hadassah Medical School, Hebrew University, Jerusalem
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Summary

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The DNAs of Shigella sonnei or Shigella dysenteriae type 1 strains isolated in outbrealis of shigellosis or in sporadic cases were analysed by restriction fragment length polymorphism (RFLP). Southern blots of the DNAs of 36 S. sonnei isolates digested by 8 restriction enzymes were hybridized with an Escherichia coli rRNA probe. The S. sonnei strains were unexpectedly diverse in their RFLP. Antibiotypes of the same isolates showed clusters of strains corresponding to the various outbreaks. On the other hand, RFLP analysis suggested concomitant multiple sources of infection rather than a common source and thereby introduced a new insight in the epidemiology of shigellosis. RFLP was also used to trace S. dysenteriae type 1 transmission in a recent cluster of clinical cases. Although antibiotic resistance patterns indicated the presence of more than one strain, RFLP analysis showed that the six isolates were identical clones and suggested the loss of an R episome after one person-to-person passage.

Type
Research Article
Information
Epidemiology & Infection , Volume 109 , Issue 2 , October 1992 , pp. 273 - 282
Copyright
Copyright © Cambridge University Press 1992

References

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