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Molecular epidemiology of Helicobacter pylori: separation of H. pylori from East Asian and non-Asian countries

Published online by Cambridge University Press:  01 February 2000

Y. YAMAOKA ,
M. S. OSATO ,
A. R. SEPULVEDA ,
O. GUTIERREZ ,
N. FIGURA ,
J. G. KIM ,
T. KODAMA ,
K. KASHIMA  and
D. Y. GRAHAM
Y. YAMAOKA
Affiliation:
VA Medical Center and Baylor College of Medicine, Houston, TX, USA Third Department of Internal Medicine, Kyoto Prefectural University of Medicine, Kyoto, Japan
M. S. OSATO
Affiliation:
VA Medical Center and Baylor College of Medicine, Houston, TX, USA
A. R. SEPULVEDA
Affiliation:
VA Medical Center and Baylor College of Medicine, Houston, TX, USA
O. GUTIERREZ
Affiliation:
Universidad Nacional de Colombia, Bogota, Colombia
N. FIGURA
Affiliation:
Institute of Internal Medicine, University of Siena, Siena, Italy
J. G. KIM
Affiliation:
Guro Hospital, Korea University College of Medicine, Seoul, Korea
T. KODAMA
Affiliation:
Third Department of Internal Medicine, Kyoto Prefectural University of Medicine, Kyoto, Japan
K. KASHIMA
Affiliation:
Third Department of Internal Medicine, Kyoto Prefectural University of Medicine, Kyoto, Japan
D. Y. GRAHAM
Affiliation:
VA Medical Center and Baylor College of Medicine, Houston, TX, USA
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Abstract

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The predominant H. pylori strain circulating among geographic locations differs with regard to the genomic structure. This study determined whether structural subtypes of the cagA 3′ repeat region could be used to identify the population of origin of H. pylori isolates. We examined 600 cagA-positive H. pylori (Colombia, 100; USA, 100; France, 100; Canada, 20; Italy, 20; Korea, 100; Japan, 100; Hong Kong, 20; Taiwan, 20; Vietnam, 20). The cagA 3′ region was amplified by PCR using primers specific to Japanese and Western 3′ cagA gene sequences. PCR using Japanese cagA primers resulted in PCR products in 99·6% of strains from East Asia but no non-Asian strains. Conversely, PCR using Western cagA primers resulted in amplicons in 100% of non-Asian strains, and only one from East Asia. cagA genotyping is useful for molecular epidemiological studies as strains can be completely separated by differences in the cagA 3′ region.

Type
Research Article
Information
Epidemiology & Infection , Volume 124 , Issue 1 , February 2000 , pp. 91 - 96
Copyright
© 2000 Cambridge University Press