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Molecular epidemiological study on tetracycline resistance R plasmids in enterohaemorrhagic Escherichia coli O157[ratio ]H7

Published online by Cambridge University Press:  01 August 1999

S. MAKINO ,
H. ASAKURA ,
T. OBAYASHI ,
T. SHIRAHATA ,
T. IKEDA  and
K. TAKESHI
S. MAKINO
Affiliation:
Department of Veterinary Microbiology, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan
H. ASAKURA
Affiliation:
Department of Veterinary Microbiology, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan
T. OBAYASHI
Affiliation:
Department of Veterinary Microbiology, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan
T. SHIRAHATA
Affiliation:
Department of Veterinary Microbiology, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan
T. IKEDA
Affiliation:
Hokkaido Institute of Public Health, Hokkaido, Japan
K. TAKESHI
Affiliation:
Hokkaido Institute of Public Health, Hokkaido, Japan
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Abstract

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Restriction patterns obtained with EcoRI and Southern hybridization were used for the differentiation of tetracycline-resistant (Tetr) R plasmids in enterobaemorrhagic Escherichia coli (EHEC) O157[ratio ]H7 isolates from a mass outbreak at a kindergarten in Obihiro-City, Hokkaido, Japan, 1996. Two kinds of Tetr R plasmids of 50 and 95 kb were detected. The 50-kb plasmids were identical to each other, while the 93-kb plasmids were of three types that were very similar to each other. The tet genes of both 50- and 95-kb R plasmids were 100% identical to the tet gene of pSC101 and all plasmids hybridized to a probe for tet. Because food-origin O157 strains were sensitive to tetracycline, we concluded that such Tetr R-plasmids might transfer to drug-sensitive O157 strains in the infected individuals.

Type
Research Article
Information
Epidemiology & Infection , Volume 123 , Issue 1 , August 1999 , pp. 25 - 30
Copyright
© 1999 Cambridge University Press