Hostname: page-component-586b7cd67f-g8jcs Total loading time: 0 Render date: 2024-11-25T08:10:02.458Z Has data issue: false hasContentIssue false
  • English
  • Français

Molecular characterization of trimethoprim resistance in Shigella sonnei in Sicily

Published online by Cambridge University Press:  15 May 2009

A. Agodi ,
C. Jones ,
E. J. Threlfall ,
M. D'Angelo  and
M. Marranzano
A. Agodi
Affiliation:
Institute of Hygiene and Preventive Medicine, University of Catania, via Biblioteca 4, 95100 Catania, Italy
C. Jones
Affiliation:
Public Health Laboratory Service, National Collection of Type Cultures, Central Public Health Laboratory, London
E. J. Threlfall
Affiliation:
Public Health Laboratory Service, Division of Enteric Pathogens, Central Public Health Laboratory, London
M. D'Angelo
Affiliation:
Institute of Hygiene and Preventive Medicine, University of Catania, via Biblioteca 4, 95100 Catania, Italy
M. Marranzano
Affiliation:
Institute of Hygiene and Preventive Medicine, University of Catania, via Biblioteca 4, 95100 Catania, Italy
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

During the 3-year period 1985–7, all strains of Shigella sonnei isolated in Catania, Sicily, showed a high level of resistance to trimethoprim (Tp) which was invariably associated with resistance to other antibiotics.

Plasmid analysis showed 18 different electropherotypes: 35 of 37 strains harboured a plasmid of 70 Megadaltons (MDa), and 29 of 37 strains a plasmid of 130 MDa.

Restriction endonuclease fingerprinting of purified 70 MDa plasmid DNA from different strains demonstrated that these plasmids were similar but not identical.

In some strains with transferable Tp resistance, DNA hybridization analysis demonstrated the presence of genes coding for the production of dihydrofolate reductase (DHFR) type V. In contrast, there was no detectable hybridization with DNA probes specific for genes coding for DHFR types I, II and IV. This is the first report of the DHFR type V gene outside Sri Lanka.

Type
Special Article
Information
Epidemiology & Infection , Volume 105 , Issue 1 , August 1990 , pp. 29 - 40
Copyright
Copyright © Cambridge University Press 1990

References

REFERENCES

1.Amyes, SGB. The success of plasmid encoded resistance genes in clinical bacteria. J Med Microbiol 1989; 28: 73–83.CrossRefGoogle ScholarPubMed
2.Sundström, L, Vinayagamoorthy, T, Sköld, O. Novel type of plasmid-borne resistance to trimethoprim. Antimicrob Agents Chemother 1987; 31: 60–6.Google Scholar
3.Wylie, BA, Amyes, SGB, Young, HK, Koornhof, HJ. Identification of a novel plasmid encoded dihydrofolate reductase mediating high level resistance to trimethoprim. J Antimicrob Chemother 1988; 22: 429–35.Google Scholar
4.Amyes, SGB, Towner, KJ, Carter, GI, Thomson, CJ, Young, HK. The type VII dihydrofolate reductase: a novel plasmid-encoded trimethoprim-resistant enzyme from Gram-negative bacteria isolated in Britain. J Antimicrob Chemother 1989; 24: 111–19.CrossRefGoogle ScholarPubMed
5.Gorbach, SL. Le diarree di origine batterica e il loro trattamento. Lancet 1988; 5: 300–5.Google Scholar
6.Gross, RJ, Threlfall, EJ, Word, LA, Rowe, B. Drug resistance in Shigella dysenteriae, S. flexneri and S. boydii in England and Wales. Increasing incidence of resistance to trimethoprim. Br Med J 1984; 288: 784–4.Google Scholar
7.Lopez-Brea, M, Collado, L, Vicente, F, Perez, Diaz JC. Increasing antimicrobial resistance of Shigella sonnei. J Antimicrob Chemother 1983; 11: 598.CrossRefGoogle ScholarPubMed
8.Delgado, R, Otero, JR. High-level resistance to trimethoprim in Shigella sonnei associated with plasmid encoded dihydrofolate reductase type I. Antimicrob Agents Chemother 1988;32: 1598–9.CrossRefGoogle ScholarPubMed
9.Bratoeva, MP, John, JF Jr. Dissemination of trimethoprim resistant clones of Shigella sonnei in Bulgaria. J Infect Dis 1989; 159: 648–53.CrossRefGoogle ScholarPubMed
10.Bannatyne, RM, Toma, S, Cheung, R, Hu, G. Resistance to trimethoprim and other antibiotics in Ontario Shigellae. Lancet 1980; i: 426.Google Scholar
11.Chun, D, Cho, DT, Seal, SY, Suh, MH, Lee, YC. R plasmids conferring multiple drug resistance from Shigella isolated in Korea. J Hyg 1984; 92: 153–60.Google Scholar
12.Shahid, NS, Rahaman, MM, Haider, K, Banu, M, Rahman, N. Changing pattern of resistant Shiga bacillus (Shigella dysenteriae type I) and Shigella flexneri in Bangladesh. J Infect Dis 1985; 152: 1114–19.CrossRefGoogle ScholarPubMed
13.Szturm-Rubinstein, S. Determination of biotype and colicinogenic character of Shigella sonnei and its epidemiological importance. Arch Immunol Ther Exp 1968; 16: 421–8.Google Scholar
14.Bauer, AM, Kirby, WMM, Sherris, JC, Turch, M. Antibiotic susceptibility testing by standardized single disk method. Am J Clin Path 1966; 45: 493–6.CrossRefGoogle ScholarPubMed
15.Ericsson, HM, Sherris, JC. Antibiotic sensitivity testing. Acta Path Microbiol Scand 1971. Suppl. 217, 9–12.Google Scholar
16.Watanabe, T. Infective heredity of multiple drug resistance in bacteria. Bacteriol Rev 1963: 27:87–115.CrossRefGoogle ScholarPubMed
17.Grant, RB, Bannatyne, RM, Shapley, AJ. Resistance to chloramphenicol and ampicillin in Salmonella typhimurium in Ontario, Canada. J Infect Dis 1976; 134: 354–61.CrossRefGoogle ScholarPubMed
18.Chabbert, YA, Scavizzi, MR, Witchitz, JL, Gerbaud, GR, Bouanchaud, DH. Incompatibility groups and the classification of fi-resistance factors. J Bacteriol 1972; 112: 666–75.Google Scholar
19.Kado, CI, Liu, ST. Rapid procedure for detection and isolation of large and small plasmids. Bacteriol 1981; 145: 1365–73.Google Scholar
20.Kraft, CA, Timbury, MC, Platt, DJ. Restriction enzyme fingerprinting of trimethoprim resistance plasmids. Epidemiol Infect 1987; 98: 241–52.Google Scholar
21.Maniatis, T, Fritsch, EF, Sambrook, J. Molecular cloning: a laboratory manual. New York: Cold Spring Harbor Laboratory, 1982.Google Scholar
22.Silhavy, TJ, Berman, ML, Enquist, LW. Experiments with gene fusions. New York: Cold Spring Harbor Laboratory, 1984.Google Scholar
23.Holmes, DS, Quigley, M. A rapid boiling method for the preparation of bacterial plasmids. Anal Biochem 1981; 114: 193–7.Google Scholar
24.Fling, ME, Richards, C. The nucleotide sequence of the trimethoprim-resistant dihydrofolate reductase gene harbored by Tn 7. Nucleic Acids Res 1983; 11: 5147–58.Google Scholar
25.Carter, GI, Towner, KJ, Slack, RCB. Rapid detection of a specific trimethoprim resistance gene using a biotinylated DNA probe. J Antimicrob Chemother 1987; 20: 335–41.CrossRefGoogle ScholarPubMed
26.Zolg, JW, Hänggi, UJ, Zachau, HG. Isolation of a small DNA fragment carrying the gene for a dihydrofolate reductase from a trimethoprim resistance factor. Mol Gen Genet 1978; 164:15–29.CrossRefGoogle ScholarPubMed
27.Towner, KJ, Young, HK, Amyes, SGB. Biotinylated DNA probes for trimethoprim-resistant dihydrofolate reductase reductase types IV and V. J Antimicrob Chemother 1988; 22:285–91.CrossRefGoogle ScholarPubMed
28.Towner, KJ. A clinical isolate of Escherichia coli owing its trimethoprim resistance to a chromosomally-located trimethoprim transposon. J Antimicrob Chemother 1981; 7: 157–62.CrossRefGoogle ScholarPubMed
29.Chatkaeomorakot, A, Echeverria, P, Taylor, DN, Scriwatana, J, Leksomboon, U. Trimethoprim-resistant Shigella and enterotoxigenic Escherichia coli strains in children in Thailand. Pediatr Infect Dis J 1987; 6: 735–9.CrossRefGoogle ScholarPubMed
30.Young, HK, Amyes, SGB. Characterization of a new transposon-mediated trimethoprim-resistant dihydrofolate reductase. Biochem Pharmacol 1985; 34: 4334–7.CrossRefGoogle ScholarPubMed
31.Broad, DF, Smith, JT. Classification of trimethoprim-resistant dihydrofolate reductases mediated by R plasmids using isoelectric focusing. Eur J Biochem 1982; 125: 617–22.CrossRefGoogle Scholar
32.Fling, ME, Walton, L, Elwell, LP. Monitoring of plasmid encoded, trimethoprim-resistant dihydrofolate reductase genes: detection of a new resistant enzyme. Antimicrob Agents Chemother 1982; 22: 882–8.CrossRefGoogle ScholarPubMed
33.Young, HK, Amyes, SGB. A new mechanism of plasmid trimethoprim resistance. Characterization of an inducible dihydrofolate reductase. J Biol Chem 1986; 261: 2503–5.CrossRefGoogle ScholarPubMed
34.Young, HK, Skurray, RA, Amyes, SGB. Plasmid mediated trimethoprim-resistance in Staphylococcus aureus. Characterization of the first Gram-positive plasmid dihydrofolate reductase (type S1). Biochem J 1987; 243: 309–12.Google Scholar