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Molecular characterization of Salmonella enterica subsp. enterica serovar Typhimurium DT1 isolates

Published online by Cambridge University Press:  26 February 2004

N. LINDQVIST ,
S. HEINIKAINEN ,
A. SIITONEN  and
S. PELKONEN
N. LINDQVIST
Affiliation:
National Veterinary and Food Research Institute, Kuopio Department, P.O. Box 92, FIN-70701 Kuopio, Finland
S. HEINIKAINEN
Affiliation:
National Veterinary and Food Research Institute, Kuopio Department, P.O. Box 92, FIN-70701 Kuopio, Finland
A. SIITONEN
Affiliation:
National Public Health Institute, Laboratory of Enteric Pathogens, Mannerheimintie 166, FIN-00300 Helsinki, Finland
S. PELKONEN
Affiliation:
National Veterinary and Food Research Institute, Kuopio Department, P.O. Box 92, FIN-70701 Kuopio, Finland
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Abstract

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Salmonella Typhimurium DT1 is endemic to Finland and has caused human outbreaks since the 1960s. Domestic DT1 isolates (n=235) from 1972 to 1999 from human cases, animals and other sources, as well as foreign DT1 isolates from human cases (n=20) were analysed by molecular methods. Pulsed-field gel electrophoresis (PFGE) yielded 38 XbaI profiles. Of these, XbaI profile 10 was seen in 49% (125/255) of the isolates. Twelve IS200 profiles were obtained; the most common IS200 profile D was seen in 64% (33/52) of the isolates. Two clusters were formed by compilation of the XbaI–, BlnI– and SpeI–PFGE and IS200 profiles and possession of the serovar-specific virulence plasmid. The major cluster contained eight IS200 profiles, including IS200 profile D and XbaI profile 10, and had no virulence plasmid, and can be regarded as typical of the endemic Typhimurium DT1 infection.

Type
Research Article
Information
Epidemiology & Infection , Volume 132 , Issue 2 , April 2004 , pp. 263 - 272
Copyright
© 2003 Cambridge University Press